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Self-assembly of P22 protein cages with polyamidoamine dendrimer and inorganic nanoparticles

Published online by Cambridge University Press:  05 December 2016

Soubantika Palchoudhury ,
Ziyou Zhou ,
Karthik Ramasamy ,
Franklin Okirie ,
Peter E. Prevelige  and
Arunava Gupta
Soubantika Palchoudhury*
Affiliation:
Department of Civil & Chemical Engineering, University of Tennessee at Chattanooga, Chattanooga, Tennessee 37403
Ziyou Zhou
Affiliation:
Center for Materials for Information Technology, The University of Alabama, Tuscaloosa, Alabama 35487
Karthik Ramasamy*
Affiliation:
Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Albuquerque, New Mexico 87185
Franklin Okirie
Affiliation:
Department of Chemical & Biological Engineering, The University of Alabama, Tuscaloosa, Alabama 35487
Peter E. Prevelige*
Affiliation:
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294
Arunava Gupta*
Affiliation:
Center for Materials for Information Technology, The University of Alabama, Tuscaloosa, Alabama 35487; and Department of Chemical & Biological Engineering, The University of Alabama, Tuscaloosa, Alabama 35487
*
a) Address all correspondence to these authors. e-mail: [email protected]
b) e-mail: [email protected]
c) e-mail: [email protected]
d) e-mail: [email protected]
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Abstract

Protein cage based nanoarchitectures hold great potential in the fields of energy, catalysis, and bio-applications owing to their ability to tune material’s properties in a benign biomimetic approach. We demonstrate the self-assembly of bacteriophage P22 using inorganic nanoparticles and dendrimers for the first time. Inorganic nanoparticles (iron oxide, CoFe2O4, and Au) and polyamidoamine serve as model systems for rigid and soft linker materials, respectively, to induce P22 assembly via electrostatic interaction. We observed distinctly different packing of P22 using nanoparticles as compared to the polyamidoamine polymer. Notably, the ratio of nanoparticle: P22 and ligand packing on the nanoparticle surface are dominant controls for this assembly. The best results are obtained at 6.5:1 nanoparticle:P22 number ratio in the presence of 50 mM NaCl, pH = 6. In contrast, dense area assembly of P22 is observed at 8:1 polyamidoamine:P22 number ratio with 1 M NaCl (pH ∼ 7.5) for the dendrimer.

Keywords

biomimeticself-assemblynanostructure
Type
Articles
Information
Journal of Materials Research , Volume 32 , Issue 2 , 27 January 2017 , pp. 465 - 472
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Copyright
Copyright © Materials Research Society 2016 

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