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Protein-functionalized WO3 nanorods–based impedimetric platform for sensitive and label-free detection of a cardiac biomarker

Published online by Cambridge University Press:  06 February 2019

Deepika Sandil ,
Suresh C. Sharma  and
Nitin K. Puri Open the ORCID record for Nitin K. Puri [Opens in a new window]
Deepika Sandil
Affiliation:
Advanced Sensor Laboratory, Department of Applied Physics, Delhi Technological University, Delhi 110042, India; and Department of Applied Physics, Bhagwan Parshuram Institute of Technology, Delhi 110089, India
Suresh C. Sharma
Affiliation:
Advanced Sensor Laboratory, Department of Applied Physics, Delhi Technological University, Delhi 110042, India
Nitin K. Puri*
Affiliation:
Advanced Sensor Laboratory, Department of Applied Physics, Delhi Technological University, Delhi 110042, India
*
a)Address all correspondence to this author. e-mail: [email protected], [email protected]
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Abstract

We report the development of a sensitive and a label-free electrochemical immunosensing platform for the detection of cardiac biomarker troponin I (cTnI) using tungsten trioxide nanorods (WO3 NRs). The low-temperature hydrothermal technique was employed for the controlled synthesis of WO3 NRs. Thin films of 3-aminopropyltriethoxy saline (APTES)-functionalized WO3 NRs were deposited on indium tin oxide (ITO)-coated glass substrate (0.5 cm × 1 cm) using electrophoretic deposition technique. The covalent immobilization of cTnI antibody onto functionalized WO3 NRs electrode was accomplished using EDC-NHS [1-(3-(dimethylamino)-propyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysulfosuccinimide] chemistry. The structural and morphological characterizations of WO3 NRs and functionalized WO3 NRs were studied using X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and electrochemical techniques. The impedimetric response study of the proposed immunosensor demonstrates high sensitivity [6.81 KΩ mL·cm2)] in a linear detection range of 0.01–10 ng/mL. The excellent selectivity, good reproducibility, and long-term stability of the proposed immunosensing platform indicate WO3 NRs as a suitable platform for the development of a point-of-care biosensing device for cardiac detection.

Keywords

chemical synthesisnanostructuresolution deposition
Type
Article
Information
Journal of Materials Research , Volume 34 , Issue 8 , 29 April 2019 , pp. 1331 - 1340
Copyright
Copyright © Materials Research Society 2019 

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