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Nanoencapsulating living biological cells using electrostatic layer-by-layer self-assembly: Platelets as a model

Published online by Cambridge University Press:  24 January 2011

Qinghe Zhao ,
Hongshuai Li  and
Bingyun Li
Qinghe Zhao
Affiliation:
Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506
Hongshuai Li
Affiliation:
Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506
Bingyun Li*
Affiliation:
Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506; WVNano Initiative, Morgantown, West Virginia 26506; and Department of Chemical Engineering, College of Engineering and Mineral Resources, West Virginia University, Morgantown, West Virginia 26506
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

In the literature, a few biological cells have been used as templates to form microcapsules of a variety of shapes and sizes. In this study, we proved the concept that living cells like platelets can be encapsulated with polyelectrolytes using electrostatic layer-by-layer self-assembly (LBL), and, most importantly, the encapsulation process did not induce activation of the platelets. Glycol-chitosan and poly-L-glutamic acid were electrostatically deposited onto platelets, and the encapsulation was confirmed using confocal laser scanning microscopy and scanning electron microscopy. Transmission electron microscopy observation further confirmed that the encapsulation process was mild and the activation of platelets was negligible. The encapsulation of living biological cells like platelets can serve as a model system in a wide range of biomedical applications including local and sustained drug delivery, immune protection of artificial tissues, and versatile artificial blood.

Keywords

BiomedicalCoatingSelf-Assembly
Type
Reviews
Information
Journal of Materials Research , Volume 26 , Issue 2: Focus Issue: Self-Assembly and Directed Assembly of Advanced Materials , 28 January 2011 , pp. 347 - 351
Copyright
Copyright © Materials Research Society 2011

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