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In vitro bioactivity and antibacterial properties of bismuth oxide modified bioactive glasses

Published online by Cambridge University Press:  04 December 2017

Sakthi Prasad S ,
Itishree Ratha ,
Tarun Adarsh ,
Akrity Anand ,
Prasanta Kumar Sinha ,
Prerna Diwan ,
Kalyandurg Annapurna  and
Kaushik Biswas
Sakthi Prasad S
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Glass Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Itishree Ratha
Affiliation:
Bioceramics and Coating Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Tarun Adarsh
Affiliation:
Ram Lal Anand College, Delhi University, New Delhi-110 021, India
Akrity Anand
Affiliation:
Bioceramics and Coating Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Prasanta Kumar Sinha
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Materials Characterization & Instrumentation Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Prerna Diwan
Affiliation:
Ram Lal Anand College, Delhi University, New Delhi-110 021, India
Kalyandurg Annapurna
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Glass Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Kaushik Biswas*
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Glass Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

Chronic osteomyelitis, a bone infection caused by bacteria, requires extensive parenteral treatments. With an aim to develop bioactive glass with antibacterial properties to resist such infections, bioactive glasses with bismuth oxide as the dopant in various amounts up to 8 wt% were prepared. X-ray diffraction patterns and Fourier-transform infrared spectra of glass samples after immersion in simulated body fluid showed the presence of hydroxyapatite (HAp) and hydroxyl carbonate apatite for all samples except with the one having Bi2O3 substitution of 8 wt%. In vitro cell proliferation by MTT assay studies using a mouse fibroblast cell line (NIH3T3) have also been carried out. Primary antimicrobial activity of the glass particles was analyzed against Escherichia coli (E. coli) using broth microdilution method which exhibited bacteriostatic effects and bactericidal properties in selected samples. The combination of bioactivity, cell proliferation, and antibacterial properties of selected Bismuth-containing bioactive glasses could be exploited in treating bone-related infections.

Keywords

In vitro bioactivityIn vitro cell proliferationMTT assayBi2O3 modified bioactive glassbroth microdilution methodSBFsilica gel layerHAp and HCA
Type
Articles
Information
Journal of Materials Research , Volume 33 , Issue 2 , 29 January 2018 , pp. 178 - 190
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Copyright
Copyright © Materials Research Society 2017 

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Footnotes

Contributing Editor: Amit Bandyopadhyay

References

REFERENCES

Hench, L.L. and Jones, J.R.: Bioactive glasses: Frontiers and challenges. Front. Bioeng. Biotechnol. 3, 1 (2015).Google Scholar
Hench, L.L.: The story of Bioglass®. J. Mater. Sci.: Mater. Med. 17, 967 (2006).Google ScholarPubMed
Andersson, Ö.H., Liu, G., Karlsson, K.H., Niemi, L., Miettinen, J., and Juhanoja, J.: In vivo behaviour of glasses in the SiO2–Na2O–CaO–P2O5–Al2O3–B2O3 system. J. Mater. Sci.: Mater. Med. 1, 219 (1990).Google Scholar
Brink, M., Turunen, T., Happonen, R-P., and Yli-Urpo, A.: Compositional dependence of bioactivity of glasses in the system Na2O–K2O–MgO–CaO–B2O3–P2O5–SiO2 . J. Biomed. Mater. Res., Part A 37, 114 (1997).Google Scholar
Lew, D.P. and Waldvogel, F.A.: Osteomyelitis. Lancet 364, 369 (2004).Google Scholar
Sakoulas, G. and Moellering, R.C.: Increasing antibiotic resistance among methicillin-resistant Staphylococcus aureus strains. Clin. Infect. Dis. 46, S360 (2008).Google Scholar
Jia, W-T., Fu, Q., Huang, W-H., Zhang, C-Q., and Rahaman, M.N.: Comparison of borate bioactive glass and calcium sulfate as implants for the local delivery of teicoplanin in the treatment of methicillin-resistant Staphylococcus aureus-induced osteomyelitis in a rabbit model. Antimicrob. Agents Chemother. 59, 7571 (2015).Google Scholar
Allan, I., Newman, H., and Wilson, M.: Antibacterial activity of particulate Bioglass® against supra-and subgingival bacteria. Biomaterials 22, 1683 (2001).CrossRefGoogle ScholarPubMed
Bellantone, M., Williams, H.D., and Hench, L.L.: Broad-spectrum bactericidal activity of Ag2O-doped bioactive glass. Antimicrob. Agents Chemother. 46, 1940 (2002).Google Scholar
Zhu, H., Hu, C., Zhang, F., Feng, X., Li, J., Liu, T., Chen, J., and Zhang, J.: Preparation and antibacterial property of silver-containing mesoporous 58S bioactive glass. Mater. Sci. Eng., C 42, 22 (2014).CrossRefGoogle ScholarPubMed
Ottomeyer, M., Mohammadkah, A., Day, D., and Westenberg, D.: Broad-spectrum antibacterial characteristics of four novel borate-based bioactive glasses. Adv. Microbiol. 6, 776 (2016).Google Scholar
Mulligan, A.M., Wilson, M., and Knowles, J.C.: Effect of increasing silver content in phosphate-based glasses on biofilms of Streptococcus sanguis . J. Biomed. Mater. Res., Part A 67, 401 (2003).CrossRefGoogle ScholarPubMed
Neel, E.A.A., Ahmed, I., Pratten, J., Nazhat, S.N., and Knowles, J.C.: Characterisation of antibacterial copper releasing degradable phosphate glass fibres. Biomaterials 26, 2247 (2005).Google Scholar
Mulligan, A.M., Wilson, M., and Knowles, J.C.: The effect of increasing copper content in phosphate-based glasses on biofilms of Streptococcus sanguis . Biomaterials 24, 1797 (2003).Google Scholar
Goh, Y-F., Alshemary, A.Z., Akram, M., Kadir, M.R.A., and Hussain, R.: In vitro characterization of antibacterial bioactive glass containing ceria. Ceram. Int. 40, 729 (2014).Google Scholar
Bierer, D.W.: Bismuth subsalicylate: History, chemistry, and safety. Rev. Infect. Dis. 12, S3 (1990).CrossRefGoogle ScholarPubMed
Lambert, J.R. and Midolo, P.: The actions of bismuth in the treatment of Helicobacter pylori infection. Aliment. Pharmacol. Ther. 11, 27 (1997).Google Scholar
Parirokh, M. and Torabinejad, M.: Mineral trioxide aggregate: A comprehensive literature review—Part I: Chemical, physical, and antibacterial properties. J. Endod. 36, 16 (2010).Google Scholar
Torabinejad, M. and Parirokh, M.: Mineral trioxide aggregate: A comprehensive literature review—Part II: Leakage and biocompatibility investigations. J. Endod. 36, 190 (2010).Google Scholar
Webster, T.J., Massa-Schlueter, E.A., Smith, J.L., and Slamovich, E.B.: Osteoblast response to hydroxyapatite doped with divalent and trivalent cations. Biomaterials 25, 2111 (2004).Google Scholar
Ciobanu, G., Bargan, A.M., and Luca, C.: New bismuth-substituted hydroxyapatite nanoparticles for bone tissue engineering. JOM 67, 2534 (2015).Google Scholar
Heid, S., Stoessel, P.R., Tauböck, T.T., Stark, W.J., Zehnder, M., and Mohn, D.: Incorporation of particulate bioactive glasses into a dental root canal sealer. Biomed. Glasses 2, 29 (2016).CrossRefGoogle Scholar
Mohn, D., Zehnder, M., Imfeld, T., and Stark, W.J.: Radio-opaque nanosized bioactive glass for potential root canal application: Evaluation of radiopacity, bioactivity and alkaline capacity. Int. Endontic. J. 43, 210 (2010).Google Scholar
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., and Yamamuro, T.: Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A–W3 . J. Biomed. Mater. Res. 24, 721 (1990).Google Scholar
Bernard, S.A., Balla, V.K., Davies, N.M., Bose, S., and Bandyopadhyay, A.: Bone cell–materials interactions and Ni ion release of anodized equiatomic NiTi alloy. Acta Biomater. 7, 1902 (2011).Google Scholar
Bodhak, S., Balla, V.K., Bose, S., Bandyopadhyay, A., Kashalikar, U., Jha, S.K., and Sastri, S.: In vitro biological and tribological properties of transparent magnesium aluminate (Spinel) and aluminum oxynitride (ALON®). J. Mater. Sci.: Mater. Med. 22, 1511 (2011).Google Scholar
Andrews, J.M.: Determination of minimum inhibitory concentrations. J. Antimicrob. Chemother. 48, 5 (2001).Google Scholar
Ou, X., Xu, S., Warnett, J.M., Holmes, S.M., Zaheer, A., Garforth, A.A., Williams, M.A., Jiao, Y., and Fan, X.: Creating hierarchies promptly: Microwave-accelerated synthesis of ZSM-5 zeolites on macrocellular silicon carbide (SiC) foams. Chem. Eng. J. 312, 1 (2017).CrossRefGoogle Scholar
El Badry, K.M., Moustaffa, F.A., Azooz, M.A., and El Batal, F.H.: Infrared absorption spectroscopy of some bio-glasses before and after immersion in various solutions. Indian J. Pure Appl. Phys. 38, 741 (2000).Google Scholar
Koutsopoulos, S.: Synthesis and characterization of hydroxyapatite crystals: A review study on the analytical methods. J. Biomed. Mater. Res. 62, 600 (2002).CrossRefGoogle Scholar
Ren, F.Z. and Leng, Y.: Carbonated apatite, type-A or type-B? Key Eng. Mater. 493, 293 (2012).Google Scholar
Ibrahim, D.M., Mostafa, A.A., and Korowash, S.I.: Chemical characterization of some substituted hydroxyapatites. Chem. Cent. J. 5, 74 (2011).Google Scholar
Ardelean, I., Cora, S., and Ioncu, V.: Structural investigation of CuO–Bi2O3–B2O3 glasses by FT-IR, Raman and UV-VIS spectroscopies. J. Optoelectron. Adv. Mater. 8, 1843 (2006).Google Scholar
He, F., He, Z., Xie, J., and Li, Y.: IR and Raman spectra properties of Bi2O3–ZnO–B2O3–BaO quaternary glass system. Am. J. Anal. Chem. 5, 1142 (2014).CrossRefGoogle Scholar
Massera, J., Fagerlund, S., Hupa, L., and Hupa, M.: Crystallization mechanism of the bioactive glasses, 45S5 and S53P4. J. Am. Ceram. Soc. 95, 607 (2012).Google Scholar
Fujikura, K., Karpukhina, N., Kasuga, T., Brauer, D.S., Hill, R.G., and Law, R.V.: Influence of strontium substitution on structure and crystallisation of Bioglass® 45S5. J. Mater. Chem. 22, 7395 (2012).Google Scholar
Wang, X., Fagerlund, S., Massera, J., Södergård, B., and Hupa, L.: Do properties of bioactive glasses exhibit mixed alkali behavior? J. Mater. Sci. 52, 8986 (2017).Google Scholar
da Silva Gasque, K.C., Al-Ahj, L.P., Oliveira, R.C., and Magalhães, A.C.: Cell density and solvent are critical parameters affecting formazan evaluation in MTT assay. Braz. Arch. Biol. Technol. 57, 381 (2014).Google Scholar
Kruse, C.R., Singh, M., Targosinski, S., Sinha, I., Sørensen, J.A., Eriksson, E., and Nuutila, K.: The effect of pH on cell viability, cell migration, cell proliferation, wound closure, and wound reepithelialization: In vitro and in vivo study. Wound Repair Regen. 25, 260 (2017).Google Scholar
Galow, A-M., Rebl, A., Koczan, D., Bonk, S.M., Baumann, W., and Gimsa, J.: Increased osteoblast viability at alkaline pH in vitro provides a new perspective on bone regeneration. Biochem. Biophys. Rep. 10, 17 (2017).Google ScholarPubMed
Thomas, F., Bialek, B., and Hensel, R.: Medical use of bismuth: The two sides of the coin. J. Clin. Toxicol. S3, 495 (2011).Google Scholar
Reynolds, P.T., Abalos, K.C., Hopp, J., and Williams, M.E.: Bismuth toxicity: A rare cause of neurologic dysfunction. Int. J. Clin. Med. 3, 46 (2012).CrossRefGoogle Scholar
Feng, Q.L., Wu, J., Chen, G.Q., Cui, F.Z., Kim, T.N., Kim, J.O., Kim, T.N., and Kim, J.O.: A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus . J. Biomed. Mater. Res. 52, 662 (2000).Google Scholar