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In vitro bioactivity and antibacterial properties of bismuth oxide modified bioactive glasses

Published online by Cambridge University Press:  04 December 2017

Sakthi Prasad S ,
Itishree Ratha ,
Tarun Adarsh ,
Akrity Anand ,
Prasanta Kumar Sinha ,
Prerna Diwan ,
Kalyandurg Annapurna  and
Kaushik Biswas
Sakthi Prasad S
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Glass Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Itishree Ratha
Affiliation:
Bioceramics and Coating Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Tarun Adarsh
Affiliation:
Ram Lal Anand College, Delhi University, New Delhi-110 021, India
Akrity Anand
Affiliation:
Bioceramics and Coating Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Prasanta Kumar Sinha
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Materials Characterization & Instrumentation Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Prerna Diwan
Affiliation:
Ram Lal Anand College, Delhi University, New Delhi-110 021, India
Kalyandurg Annapurna
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Glass Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Kaushik Biswas*
Affiliation:
Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Glass Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

Chronic osteomyelitis, a bone infection caused by bacteria, requires extensive parenteral treatments. With an aim to develop bioactive glass with antibacterial properties to resist such infections, bioactive glasses with bismuth oxide as the dopant in various amounts up to 8 wt% were prepared. X-ray diffraction patterns and Fourier-transform infrared spectra of glass samples after immersion in simulated body fluid showed the presence of hydroxyapatite (HAp) and hydroxyl carbonate apatite for all samples except with the one having Bi2O3 substitution of 8 wt%. In vitro cell proliferation by MTT assay studies using a mouse fibroblast cell line (NIH3T3) have also been carried out. Primary antimicrobial activity of the glass particles was analyzed against Escherichia coli (E. coli) using broth microdilution method which exhibited bacteriostatic effects and bactericidal properties in selected samples. The combination of bioactivity, cell proliferation, and antibacterial properties of selected Bismuth-containing bioactive glasses could be exploited in treating bone-related infections.

Keywords

In vitro bioactivityIn vitro cell proliferationMTT assayBi2O3 modified bioactive glassbroth microdilution methodSBFsilica gel layerHAp and HCA
Type
Articles
Information
Journal of Materials Research , Volume 33 , Issue 2 , 29 January 2018 , pp. 178 - 190
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Copyright
Copyright © Materials Research Society 2017 

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Footnotes

Contributing Editor: Amit Bandyopadhyay

References

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