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A biomimetic strategy for controllable degradation of chitosan scaffolds

Published online by Cambridge University Press:  13 June 2012

Yuangang Liu ,
Changren Zhou  and
Yan Sun
Yuangang Liu*
Affiliation:
Department of Chemical Engineering and Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China
Changren Zhou*
Affiliation:
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China
Yan Sun
Affiliation:
Department of Biotechnology, Guangdong Pharmaceutical University 510006, China
*
a)Address all correspondence to these authors. e-mail: [email protected]
b)e-mail: [email protected]
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Abstract

Controllable degradation of scaffolds plays an important role in tissue engineering applications. Here, we describe a biomimetic approach to control chitosan scaffold degradation by incorporating lysozyme-loaded poly(D,L-lactic-co-glycolic acid) microspheres in 3D chitosan scaffolds. In vitro degradation tests reveal that the degradation rate increased when the mass ratio of microspheres-to-chitosan increased whereas the contrast group showed a visible turning point at 28d. In vivo degradation rate was much faster than that in vitro, and the relationship between in vitro degradation and in vivo degradation was correlative. Finally, for determining the primary biocompatibility of the combined scaffolds, studies such as cytotoxicity assay, cell attachment study and histological evaluation were carried out. It is concluded that the combination method of enzyme and scaffold is suitable for chitosan scaffold degradation; it also demonstrates an alternative strategy for other biomaterials used in tissue engineering.

Keywords

biomimeticcompositetissue
Type
Articles
Information
Journal of Materials Research , Volume 27 , Issue 14 , 28 July 2012 , pp. 1859 - 1868
Copyright
Copyright © Materials Research Society 2012

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