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Fabrication of thermoplastic polyurethane tissue engineering scaffold by combining microcellular injection molding and particle leaching

Published online by Cambridge University Press:  16 April 2014

Hao-Yang Mi ,
Xin Jing ,
Max R. Salick ,
Lih-Sheng Turng  and
Xiang-Fang Peng
Hao-Yang Mi
Affiliation:
School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou 510640, China; and Department of Mechanical Engineering, University of Wisconsin–Madison, Madison, Wisconsin 53706
Xin Jing
Affiliation:
National Engineering Research Center of Novel Equipment for Polymer Processing, South China University of Technology, Guangzhou 510640, China; and Department of Mechanical Engineering, University of Wisconsin–Madison, Madison, Wisconsin 53706
Max R. Salick
Affiliation:
Department of Engineering Physics, University of Wisconsin–Madison, Madison, Wisconsin 53706
Lih-Sheng Turng*
Affiliation:
Department of Mechanical Engineering, University of Wisconsin–Madison, Madison, Wisconsin 53706
Xiang-Fang Peng*
Affiliation:
National Engineering Research Center of Novel Equipment for Polymer Processing, South China University of Technology, Guangzhou 510640, China
*
a)Address all correspondence to these authors. e-mail: [email protected]
b)e-mail: [email protected]
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Abstract

Microcellular injection molding, a process capable of mass-producing complex plastic parts, and particle leaching methods were combined to fabricate porous thermoplastic polyurethane tissue engineering scaffolds. Water soluble polyvinyl alcohol (PVOH) and sodium chloride (NaCl) were used as porogens to improve the porosity and interconnectivity as well as the hydrophilicity of the scaffolds. It was found in the study that the microcellular injection molding process was effective at producing high pore density and porosity. The addition of PVOH decreased the pore diameter and increased the pore density. Furthermore, scaffolds with NaCl and PVOH porogens showed more interconnected pores. The 3T3 fibroblast cell culture was used to confirm the biocompatibility of the scaffolds. Residual PVOH content after leaching increased the hydrophilicity of the scaffolds and further improved cell adhesion and proliferation. The resulting scaffolds offer an alternative scalable tissue scaffold fabrication method for soft tissue scaffold production.

Keywords

microcellular injection moldingparticle leachingthermoplastic polyurethanetissue engineering scaffold
Type
Articles
Information
Journal of Materials Research , Volume 29 , Issue 8 , 28 April 2014 , pp. 911 - 922
Copyright
Copyright © Materials Research Society 2014 

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References

REFERENCES

Wang, X.W., Lin, P., Yao, Q.H., and Chen, C.Y.: Development of small-diameter vascular grafts. World J. Surg. 31, 682 (2007).CrossRefGoogle ScholarPubMed
Jang, D.W., Nguyen, T.H., Sarkar, S.K., and Lee, B.T.: Microwave sintering and in vitro study of defect-free stable porous multilayered HAp-ZrO2 artificial bone scaffold. Sci. Technol. Adv. Mat. 13, (2012).CrossRefGoogle Scholar
Li, B., Davidson, J.M., and Guelcher, S.A.: The effect of the local delivery of platelet-derived growth factor from reactive two-component polyurethane scaffolds on the healing in rat skin excisional wounds. Biomaterials 30, 3486 (2009).CrossRefGoogle ScholarPubMed
Tian, Z.C., Zhu, Y.L., Qiu, J.J., Guan, H.F., Li, L.Y., Zheng, S.C., Dong, X.H., and Xiao, J.: Synthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for bone regeneration. J. Huazhong U. Sci.-Med. 32, 563 (2012).CrossRefGoogle ScholarPubMed
Rosado, A.M. and Brewster, L.P.: Regeneration: Letting the scaffold do the work. J. Surg. Res. 180, 49 (2013).CrossRefGoogle ScholarPubMed
Zhang, Y.Z., Venugopal, J., Huang, Z.M., Lim, C.T., and Ramakrishna, S.: Characterization of the surface biocompatibility of the electrospun PCL-collagen nanofibers using fibroblasts. Biomacromolecules 6, 2583 (2005).CrossRefGoogle ScholarPubMed
Yang, S.F., Leong, K.F., Du, Z.H., and Chua, C.K.: The design of scaffolds for use in tissue engineering. Part 1. Traditional factors. Tissue Eng. 7, 679 (2001).CrossRefGoogle Scholar
Gupta, B., Patra, S., and Ray, A.R.: Preparation of porous polycaprolactone tubular matrix by salt leaching process. J. Appl. Polym. Sci. 126, 1505 (2012).CrossRefGoogle Scholar
Sherwood, J.K., Riley, S.L., Palazzolo, R., Brown, S.C., Monkhouse, D.C., Coates, M., Griffith, L.G., Landeen, L.K., and Ratcliffe, A.: A three-dimensional osteochondral composite scaffold for articular cartilage repair. Biomaterials 23, 4739 (2002).CrossRefGoogle ScholarPubMed
Shao, X.X., Hutmacher, D.W., Ho, S.T., Goh, J.C.H., and Lee, E.H.: Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits. Biomaterials 27, 1071 (2006).CrossRefGoogle ScholarPubMed
Odedra, D., Chiu, L., Reis, L., Rask, F., Chiang, K., and Radisic, M.: Cardiac tissue engineering. In Biomaterials for Tissue Engineering Applications: A Review of the Past and Future Trends, Burdick, J.A. and Mauck, R.L. ed.; Springer, New York, 2011; p. 421.CrossRefGoogle Scholar
Liu, X.H. and Ma, P.X.: Polymeric scaffolds for bone tissue engineering. Ann. Biomed. Eng. 32, 477 (2004).CrossRefGoogle ScholarPubMed
Ghasemi-Mobarakeh, L., Prabhakaran, M.P., Morshed, M., Nasr-Esfahani, M.H., and Ramakrishna, S.: Bio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering. Mat. Sci. Eng. C-Mater. 30, 1129 (2010).CrossRefGoogle Scholar
Shor, L., Yildirim, E.D., Guceri, S., and Sun, W.: Precision extruding deposition for freeform fabrication of PCL and PCL-HA tissue scaffolds. Biological and Medical Physics. Biomedical Engineering, (Springer-Verlag, New York, 2010); p. 91.Google Scholar
Yeo, A., Wong, W.J., and Teoh, S.H.: Surface modification of PCL-TCP scaffolds in rabbit calvaria defects: Evaluation of scaffold degradation profile, biomechanical properties and bone healing patterns. J. Biomed. Mater. Res. A 93, 1358 (2010).CrossRefGoogle ScholarPubMed
Ajami-Henriquez, D., Rodriguez, M., Sabino, M., Castillo, R.V., Muller, A.J., Boschetti-de-Fierro, A., Abetz, C., Abetz, V., and Dubois, P.: Evaluation of cell affinity on poly(L-lactide) and poly(epsilon-caprolactone) blends and on PLLA-b-PCL diblock copolymer surfaces. J. Biomed. Mater. Res. A 87, 405 (2008).CrossRefGoogle ScholarPubMed
Navarro, M., Aparicio, C., Charles-Harris, M., Ginebra, M.P., Engel, E., and Planell, J.A.: Development of a biodegradable composite scaffold for bone tissue engineering: Physicochemical, topographical, mechanical, degradation, and biological properties. Adv. Polym. Sci. 200, 209 (2006).CrossRefGoogle Scholar
Nieponice, A., Soletti, L., Guan, J.J., Hong, Y., Gharaibeh, B., Maul, T.M., Huard, J., Wagner, W.R., and Vorp, D.A.: In Vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model. Tissue Eng. Pt. A 16, 1215 (2010).CrossRefGoogle ScholarPubMed
Danielsson, C., Ruault, S., Simonet, M., Neuenschwander, P., and Frey, P.: Polyesterurethane foam scaffold for smooth muscle cell tissue engineering. Biomaterials 27, 1410 (2006).CrossRefGoogle ScholarPubMed
Hentschel, T. and Munstedt, H.: Thermoplastic polyurethane - the material used for the Erlanger silver catheter. Infection 27, S43 (1999).CrossRefGoogle ScholarPubMed
Maurus, P.B. and Kaeding, C.C.: Bioabsorbable implant material review. Oper. Techn. Sport. Med. 12, 158 (2004).CrossRefGoogle Scholar
Lamba, N., Woodhouse, K., and Cooper, S.: Polyurethanes in Biomedical Applications (CRC Press, New York, 1998).Google Scholar
Huang, C., Chen, R., Ke, Q.F., Morsi, Y., Zhang, K.H., and Mo, X.M.: Electrospun collagen-chitosan-TPU nanofibrous scaffolds for tissue engineered tubular grafts. Colloid Surface B 82, 307 (2011).CrossRefGoogle ScholarPubMed
Dempsey, D.K., Schwartz, C.J., Ward, R.S., Iyer, A.V., Parakka, J.P., and Cosgriff-Hernandez, E.M.: Micropatterning of electrospun polyurethane fibers through control of surface topography. Macromol. Mater. Eng. 295, 990 (2010).CrossRefGoogle Scholar
Martinez-Perez, C.A., Garcia-Casillas, P.E., Romero, P., Martinez-Villafane, A., Moller, A.D., and Romero-Garcia, J.: Porous biodegradable polyurethane scaffolds prepared by thermally induced phase separation. J. Adv. Mater.-Covina 1, 5 (2006).Google Scholar
Sin, D., Miao, X.G., Liu, G., Wei, F., Chadwick, G., Yan, C., and Friis, T.: Polyurethane (PU) scaffolds prepared by solvent casting/particulate leaching (SCPL) combined with centrifugation. Mat. Sci. Eng. C 30, 78 (2010).CrossRefGoogle Scholar
He, K. and Wang, X.H.: Rapid prototyping of tubular polyurethane and cell/hydrogel constructs. J. Bioact. Compat. Pol. 26, 363 (2011).Google Scholar
Ito, S., Matsunaga, K., Tajima, M., and Yoshida, Y.: Generation of microcellular polyurethane with supercritical carbon dioxide. J. Appl. Polym. Sci. 106, 3581 (2007).CrossRefGoogle Scholar
Leicher, S., Will, J., Haugen, H., and Wintermantel, E.: MuCell (R) technology for injection molding: A processing method for polyether-urethane scaffolds. J. Mater. Sci. 40, 4613 (2005).CrossRefGoogle Scholar
Gerhardt, L.J., Manke, C.W., and Gulari, E.: Rheology of polydimethylsiloxane swollen with supercritical carbon dioxide. J. Polym. Sci. Pol. Phys. 35, 523 (1997).3.0.CO;2-J>CrossRefGoogle Scholar
Wu, L.B., Jing, D.Y., and Ding, J.D.: A “room-temperature” injection molding/particulate leaching approach for fabrication of biodegradable three-dimensional porous scaffolds. Biomaterials 27, 185 (2006).CrossRefGoogle Scholar
Liu, S.J., Hsueh, C.L., Ueng, S.W.N., Lin, S.S., and Chen, J.K.: Manufacture of solvent-free polylactic-glycolic acid (PLGA) scaffolds for tissue engineering. Asia-Pac. J. Chem. Eng. 4, 154 (2009).CrossRefGoogle Scholar
Kramschuster, A. and Turng, L.S.: An injection molding process for manufacturing highly porous and interconnected biodegradable polymer matrices for use as tissue engineering scaffolds. J. Biomed. Mater. Res. B. 92, 366 (2010).CrossRefGoogle ScholarPubMed
Naguib, H.E. and Park, C.B.: Strategies for achieving ultra low-density polypropylene foams. Polym. Eng. Sci. 42, 1481 (2002).CrossRefGoogle Scholar
Gong, P.J. and Ohshima, M.: The effect of interfacial miscibility on the cell morphology of polyethylene terephthalate/bisphenol a polycarbonate blend foams. J. Polym. Sci. Pol. Phys. 50, 1173 (2012).CrossRefGoogle Scholar
Leung, S.N., Park, C.B., and Li, H.: Numerical simulation of polymeric foaming processes using modified nucleation theory. Plast. Rubber Compos. 35, 93 (2006).CrossRefGoogle Scholar
Oh, S.H., Kang, S.G., Kim, E.S., Cho, S.H., and Lee, J.H.: Fabrication and characterization of hydrophilic poly(lactic-co-glycolic acid)/poly(vinyl alcohol) blend cell scaffolds by melt-molding particulate-leaching method. Biomaterials 24, 4011 (2003).CrossRefGoogle Scholar
O'Brien, F.J., Harley, B.A., Yannas, I.V., and Gibson, L.J.: The effect of pore size on cell adhesion in collagen-GAG scaffolds. Biomaterials 26, 433 (2005).CrossRefGoogle ScholarPubMed