Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-24T08:55:59.189Z Has data issue: false hasContentIssue false
  • English
  • Français

Cell labeling efficiency of layer-by-layer self-assembly modified silica nanoparticles

Published online by Cambridge University Press:  31 January 2011

Gang Liu ,
Jing Tian ,
Chen Liu ,
Hua Ai ,
Zhongwei Gu ,
Jilong Gou  and
Xianming Mo
Gang Liu
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Jing Tian
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Chen Liu
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Hua Ai*
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Zhongwei Gu
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Jilong Gou
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Xianming Mo
Affiliation:
Laboratory of Stem Cell Biology, West China Medical School, Sichuan University, Chengdu 610041, People’s Republic of China
*
a) Address all correspondence to this author. e-mail: [email protected]
Get access

Abstract

In the present study, we compared cytotoxicity and cell uptake of silica nanoparticles with four different surface coatings generated through layer-by-layer self-assembly. Rabbit mesenchymal stem cells (rMSCs) were labeled with silica nanoparticles of different coatings including poly(ethyleneimine) (PEI), poly(allylamine hydrochloride) (PAH), poly(anetholesulfonic acid, sodium salt) (PAS), and dextran sulfate. The MTT [3-(4, 5-dimethylthiazol-2)-2, 5-diphenyl-2H-tetrazolium bromide] test was performed to quantify the cell biocompatibility. The cellular uptake of those silica nanoparticles was determined by flow cytometry and confocal laser scanning microscopy. The results showed that all examined silica nanoparticles were stable in aqueous phase with high monodispersity. Labeled rMSCs are unaffected in their viability, apoptosis, and differentiation capacities. The silica nanoparticle-coated synthetic polycations such as PEI or PAH have higher cell internalization than negatively charged polyelectrolytes. The ability to control cell uptake of different particles may have applications in cell labeling, cell separation, and other biomedical applications.

Keywords

BiomaterialCellular (material form)Self-assembly
Type
Articles
Information
Journal of Materials Research , Volume 24 , Issue 4 , April 2009 , pp. 1317 - 1321
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Li, Z., Suzuki, Y., Huang, M., Cao, F., Xie, X., Connolly, A.J., Yang, P.C., and Wu, J.C.: Comparison of reporter gene and iron particle labeling for tracking fate of human embryonic stem cells and differentiated endothelial cells in living subjects. Stem Cells 26, 864 (2008).CrossRefGoogle ScholarPubMed
2Lu, C.W., Hung, Y., Hsiao, J.K., Yao, M., Chung, T.H., Lin, Y.S., Wu, S.H., Hsu, S.C., Liu, H.M., Mou, C.Y., Yang, C.S., Huang, D.M., and Chen, Y.C.: Bifunctional magnetic silica nano-particles for highly efficient human stem cell labeling. Nano Lett. 7, 149 (2007).CrossRefGoogle Scholar
3Ai, H., Pink, J.J., Shuai, X., Boothman, D.A., and Gao, J.: Interactions between self-assembled polyelectrolyte shells and tumor cells. J. Biomed. Mater. Res. 73, 303 (2005).CrossRefGoogle ScholarPubMed
4Jiao, Y.P. and Cui, F.Z.: Surface modification of polyester biomaterials for tissue engineering. Biomed. Mater. 2, R24 (2007).CrossRefGoogle ScholarPubMed
5Chung, T.H., Wu, S.H., Yao, M., Lu, C.W., Lin, Y.S., Hung, Y., Mou, C.Y., Chen, Y.C., and Huang, D.M.: The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells. Biomaterials 28, 2959 (2007).CrossRefGoogle ScholarPubMed
6Lorenz, M.R., Holzapfel, V., Musyanovych, A., Nothelfer, K., Walther, P., Frank, H., Landfester, K., Schrezenmeier, H., and Mailänder, V.: Uptake of functionalized, fluorescent-labeled polymeric particles in different cell lines and stem cells. Biomaterials 27, 2820 (2006).CrossRefGoogle ScholarPubMed
7Bentzen, E.L., Tomlinson, I.D., Mason, J., Gresch, P., Warnement, M.R., Wright, D., Sanders-Bush, E., Blakely, R., and Rosenthal, S.J.: Surface modification to reduce nonspecific binding of quantum dots in live cell assays. Bioconjugate Chem. 16, 1488 (2005).CrossRefGoogle ScholarPubMed
8Lewin, M., Carlesso, N., Tung, C.H., Tang, X.W., Cory, D., Scadden, D.T., and Weissleder, R.: Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat. Biotechnol. 18, 410 (2000).CrossRefGoogle ScholarPubMed
9He, X., Duan, J., Wang, K., Tan, W., Lin, X., and He, C.: A novel fluorescent label based on organic dye-doped silica nanoparticles for HepG liver cancer cell recognition. J. Nanosci. Nanotechnol. 4, 585 (2004).CrossRefGoogle ScholarPubMed
10Koch, A.M., Reynolds, F., Kircher, M.F., Merkle, H.P., Weissleder, R., and Josephson, L.: Uptake and metabolism of a dual fluorochrome Tat-nanoparticle in HeLa cells. Bioconjugate Chem. 14, 1115 (2003).CrossRefGoogle ScholarPubMed
11Liu, T.C., Zhang, H.L., Wang, J.H., Wang, H.Q., Zhang, Z.H., Hua, X.F., Cao, Y.C., Luo, Q.M., and Zhao, Y.D.: Study on molecular interactions between proteins on live cell membranes using quantum dot-based fluorescence resonance energy transfer. Anal. Bioanal. Chem. 391, 2819 (2008).CrossRefGoogle Scholar
12Jia, N., Lian, Q., Shen, H., Wang, C., Li, X., and Yang, Z.: Intracellular delivery of quantum dots tagged antisense oligodeoxynucleotides by functionalized multiwalled carbon nanotubes. Nano Lett. 7, 2976 (2007).CrossRefGoogle ScholarPubMed
13Soto, E.R. and Ostroff, G.R.: Characterization of multilayered nanoparticles encapsulated in yeast cell wall particles for DNA delivery. Bioconjugate Chem. 19, 840 (2008).CrossRefGoogle ScholarPubMed
14Chang, S.Y., Liu, L., and Asher, S.A.: Preparation and properties of tailored morphology, monodisperse colloidal silica-cadmium sulfide nanocomposites. J. Am. Chem. Soc. 116, 6739 (1994).CrossRefGoogle Scholar
15Wang, Z.Y., Liu, G., Sun, J.Y., Gong, Q.Y., Song, B., Ai, H., and Gu, Z.W.: Self-assembly of magnetite nanocrystals with amphiphilic polyethylenimine: Structures and applications in magnetic resonance imaging. J. Nanosci. Nanotechnol. 9, 378 (2009).CrossRefGoogle Scholar
16Qua, Z.H., Zhang, X.L., Tang, T.T., and Dai, K.R.: Promotion of osteogenesis through b-catenin signaling by desferrioxamine. Biochem. Biophys. Res. Commun. 370, 332 (2008).CrossRefGoogle Scholar
17Ai, H., Jones, S.A., and Lvov, Y.M.: Biomedical applications of electrostatic layer-by-layer nano-assembly of polymers, enzymes, and nanoparticles. Cell Biochem. Biophys. 39, 23 (2003).CrossRefGoogle ScholarPubMed
18Thierry, B., Winnik, F.M., Merhi, Y., Silver, J., and Tabrizian, M.: Bioactive coatings of endovascular stents based on polyelectrolyte multilayers. Biomacromolecules 4, 1564 (2003).CrossRefGoogle ScholarPubMed
19Tiourina, O.P. and Sukhorukov, G.B.: Multilayer alginate/protamine microsized capsules: Encapsulation of alpha-chymotrypsin and controlled release study. Int. J. Pharm. 242, 155 (2002).CrossRefGoogle ScholarPubMed
20Georgieva, R., Moya, S., Hin, M., Mitlöhner, R., Donath, E., Kiesewetter, H., Möhwald, H., and Bäumler, H.: Permeation of macromolecules into polyelectrolyte microcapsules. Biomacro-molecules 3, 517 (2002).CrossRefGoogle ScholarPubMed
21Fischer, H.C. and Chan, W.C.: Nanotoxicity: The growing need for in vivo study. Curr. Opin. Biotechnol. 18, 565 (2007).CrossRefGoogle ScholarPubMed
22Stone, V., Johnston, H., and Clift, M.J.: Air pollution, ultrafine and nanoparticle toxicology: Cellular and molecular interactions. IEEE Trans. Nanobioscience 6, 331 (2007).CrossRefGoogle ScholarPubMed