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Antitumor activity and antitumor mechanism of triphenylphosphonium chitosan against liver carcinoma

Published online by Cambridge University Press:  14 August 2018

Haochao Huang Open the ORCID record for Haochao Huang [Opens in a new window] ,
Haiwei Wu ,
Yongrui Huang ,
Shuangying Zhang ,
Yuetwai Lam  and
Ningjian Ao
Haochao Huang
Affiliation:
Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China
Haiwei Wu
Affiliation:
College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China
Yongrui Huang
Affiliation:
Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China
Shuangying Zhang
Affiliation:
Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China
Yuetwai Lam
Affiliation:
Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China
Ningjian Ao*
Affiliation:
Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

N-(3-Carboxypropyl) triphenylphosphonium bromide chitosan (TPPB-CS) was synthesized and characterized by FTIR, 1H NMR spectrometer, and Zeta potential. TPPB-CS showed a selectivity-toxicity among cancer cell lines (MG-63 and HepG2 cells) and mouse embryonic fibroblast cells (NIH3T3 cells). A significant effect on inhibiting cell migration in HepG2 cells was observed in vitro, and TPPB-CS could effectively inhibit tumor growth in H22-bearing mice in vivo. Furthermore, the distribution of cell cycle, the level of reactive oxygen species (ROS), mitochondrial transmembrane potential (∆ψm), the expression of tumor necrosis factor α (TNF-α), and vascular endothelial growth factor (VEGF) were examined to investigate the antitumor mechanism of TPPB-CS. The results suggested that the antitumor activity of TPPB-CS may be attributed to delay the cell cycle in S phase, alter the ROS and ∆ψm level, as well as regulate the TNF-α and VEGF secretion. TPPB-CS can become a promising anticancer drug for clinical therapy.

Keywords

triphenylphosphonium chitosanantitumor activity and mechanismliver carcinoma
Type
Article
Information
Journal of Materials Research , Volume 33 , Issue 17 , 14 September 2018 , pp. 2586 - 2597
Copyright
Copyright © Materials Research Society 2018 

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