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Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery

Published online by Cambridge University Press:  15 August 2012

B Levin ,
S L Redmond ,
R Rajkhowa ,
R H Eikelboom ,
M D Atlas  and
R J Marano
B Levin*
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia Sir Charles Gairdner Hospital, Perth, Western Australia, Australia
S L Redmond
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia
R Rajkhowa
Affiliation:
Centre for Material and Fibre Innovation, Deakin University, Geelong, Victoria, Australia
R H Eikelboom
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia
M D Atlas
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia Sir Charles Gairdner Hospital, Perth, Western Australia, Australia St John of God Hospital, Perth, Western Australia, Australia
R J Marano
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia
*
Address for correspondence: Dr Brett Levin, Ear Sciences Centre, School of Surgery, Suite 3, 1 Salvado Rd, Subiaco, WA, 6008 Fax: +61 (02) 9845 9852 E-mail: [email protected]
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Abstract

Background:

Chronic tympanic membrane perforations can cause significant morbidity. The term myringoplasty describes the operation used to close such perforations. A variety of graft materials are available for use in myringoplasty, but all have limitations and few studies report post-operative hearing outcomes. Recently, the biomedical applications of silk fibroin protein have been studied. This material's biocompatibility, biodegradability and ability to act as a scaffold to support cell growth prompted an investigation of its interaction with human tympanic membrane keratinocytes.

Methods and materials:

Silk fibroin membranes were prepared and human tympanic membrane keratinocytes cultured. Keratinocytes were seeded onto the membranes and immunostained for a number of relevant protein markers relating to cell proliferation, adhesion and specific epithelial differentiation.

Results:

The silk fibroin scaffolds successfully supported the growth and adhesion of keratinocytes, whilst also maintaining their cell lineage.

Conclusion:

The properties of silk fibroin make it an attractive option for further research, as a potential alternative graft in myringoplasty.

Keywords

Tympanic Membrane PerforationMyringoplastySilk FibroinGraftKeratinocyte
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Supplement S1 , January 2013 , pp. S13 - S20
Copyright
Copyright © JLO (1984) Limited 2012

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