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Tacrolimus enhances the recovery of normal laryngeal muscle fibre distribution after reinnervation

Published online by Cambridge University Press:  04 September 2012

P Gorphe ,
N Guerout ,
M Birchall ,
G Terenghi  and
J-P Marie
P Gorphe*
Affiliation:
Otorhinolaryngology and Head and Neck Surgery Department, Rouen University Hospital, France Experimental Surgery Laboratory, Faculty of Medicine, Rouen University, France Otorhinolaryngology and Head and Neck Surgery Department, Hôpital Avicenne, Bobigny, France
N Guerout
Affiliation:
Experimental Surgery Laboratory, Faculty of Medicine, Rouen University, France
M Birchall
Affiliation:
UCL Ear Hospital, University College London, UK
G Terenghi
Affiliation:
Blond McIndoe Laboratories, Tissue Injury and Repair Group, School of Medicine, University of Manchester, UK
J-P Marie
Affiliation:
Otorhinolaryngology and Head and Neck Surgery Department, Rouen University Hospital, France Experimental Surgery Laboratory, Faculty of Medicine, Rouen University, France
*
Address for correspondence: Dr Philippe Gorphe, Otorhinolaryngology and Head and Neck Surgery Department, Rouen University Hospital, 76031 Rouen, France E-mail: [email protected]
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Abstract

Objectives:

To assess the recovery of various muscle fibre types in the posterior cricoarytenoid muscle after laryngeal reinnervation in the rat, and to determine the influence of tacrolimus on this process.

Methods:

Four groups of rats underwent resection and anastomosis of the left vagus nerve, and were administered either tacrolimus at a low dose or an immunosuppressive dose, or cyclosporin A at a low dose or an immunosuppressive dose. A fifth group received surgery alone, and a sixth group received neither surgery nor drug treatment (healthy group). Muscles were removed for immunohistochemical analysis 45 days after surgery.

Results:

There was no difference in the proportion of types 1, 2a and 2b muscle fibres, comparing the immunosuppressive tacrolimus group and the healthy group, whereas there were fewer type 1 fibres in the group receiving surgery alone, compared with the healthy group (7 vs 12.1 per cent, respectively; p = 0.0303).

Conclusion:

Tacrolimus enhanced the recovery of normal laryngeal muscle fibres after reinnervation in the rat, indicating a possible role in laryngeal transplantation.

Keywords

TacrolimusLaryngeal ReinnervationCalcineurin InhibitorsMuscle Fibers
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 11 , November 2012 , pp. 1155 - 1158
Copyright
Copyright © JLO (1984) Limited 2012

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Footnotes

Presented in part at the Congress of the European Laryngological Society, 1st September 2006, Nottingham, UK

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