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Single dose intratympanic mesna application inhibits propylene glycol induced cholesteatoma formation

Published online by Cambridge University Press:  20 December 2016

O Ismi ,
Y Y Karabulut ,
K K Bal ,
Y Vayisoglu  and
M Unal
O Ismi*
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
Y Y Karabulut
Affiliation:
Department of Pathology, Faculty of Medicine, University of Mersin, Turkey
K K Bal
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
Y Vayisoglu
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
M Unal
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
*
Address for correspondence: Dr O Ismi, Mersin Üniversitesi Tıp Fakültesi Kulak Burun Boğaz A D, Çiftlikköy, Mezitli, Mersin, Turkey E-mail: [email protected]
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Abstract

Objective:

Mesna (i.e. sodium 2-mercaptoethanesulfonate; C2H5NaO3S2) has been used in otological surgery such as cholesteatoma dissection and tympanic membrane lateralisation in atelectatic ears. However, this study aimed to investigate its effect on cholesteatoma formation.

Methods:

A total of 20 Wistar rats were divided into two groups of 10 animals. The right and left ears of control animals were treated with saline (saline control group; n = 10 ears) and propylene glycol plus saline (propylene glycol control group; n = 10 ears), respectively. In the mesna group, both ears were treated with propylene glycol plus mesna (n = 20 ears). On days 1, 8 and 15, the saline control group had intratympanic injections of 0.2 ml saline and the propylene glycol control and mesna groups had intratympanic injections of 0.2 ml 100 per cent propylene glycol. On day 22, the propylene glycol control group had a single intratympanic injection of 0.2 ml saline and the mesna group had a single intratympanic injection of 10 per cent mesna. Animals were killed 12 weeks after the last injection and the temporal bones were sent for histopathological evaluation.

Results:

The cholesteatoma formation rate was 88 per cent in the propylene glycol control group, but was significantly lower in the mesna group (p = 0.01). There were no significant differences in granulation tissue formation (p = 0.498), cyst formation in the bulla (p = 0.381), fibrosis (p = 0.072) and epithelial hyperplasia (p = 0.081) among experimental groups.

Conclusion:

Intratympanic propylene glycol administration is an effective method of promoting experimental cholesteatoma formation. Administration of a single dose of intratympanic mesna inhibited cholesteatoma formation in an animal model.

Keywords

MesnaCholesteatomaPropylene GlycolOtitis Media
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 131 , Issue 3 , March 2017 , pp. 215 - 220
Copyright
Copyright © JLO (1984) Limited 2016 

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References

1 Sudhoff, H, Liebehenz, Y, Aschenbrenner, J, Jung, J, Hildmann, H, Dazert, S. A murine model of cholesteatoma-induced bone resorption using autologous dermal implantation. Laryngoscope 2003;113:1022–6Google Scholar
2 Choufani, G, Roper, N, Delbrouck, C, Hassid, S, Gabius, HJ. Animal model for cholesteatoma induced in the gerbil: will the profiles of differentiation/growth-regulatory markers be similar to the clinical situation? Laryngoscope 2007;117:706–11Google Scholar
3 Kuo, CL, Etiopathogenesis of acquired cholesteatoma: prominent theories and recent advances in biomolecular research. Laryngoscope 2015;125:234–40CrossRefGoogle ScholarPubMed
4 Ismi, O, Unal, M. Experimental models of cholesteatoma: a review. World J Otorhinolaryngol 2014;4:23–7CrossRefGoogle Scholar
5 Wright, CG, Bird, LL, Meyerhoff, WL. Effect of 5-fluorouracil in cholesteatoma development in an animal model. Am J Otolaryngol 1991;12:133–8Google Scholar
6 Antunes, ML, Fukuda, Y, Penido Nde, O, Ferreira, R. Effect of trans-retinoic acid in the inhibition of cholesteatoma in guinea pigs. Braz J Otorhinolaryngol 2008;74:53–60 CrossRefGoogle Scholar
7 Melo, AA, Caldas Neto, SS, Leão, FS, Campos, AJC. Effect of intratympanic mitomycin C on the development of cholesteatoma and otitis media in rats. J Laryngol Otol 2013;127:359–63Google Scholar
8 Sennaroglu, L, Ozkul, A, Gedikoglu, G, Ergin, T. Effect of intratympanic steroid application on the development of experimental cholesteatoma. Laryngoscope 1998;108:543–7CrossRefGoogle ScholarPubMed
9 Kayhan, FT, Algun, Z. The effect of systemic prednisolone on propylene-glycol induced otitis media in guinea pig. Kulak Burun Bogaz Ihtis Derg 2006;16:214–20Google ScholarPubMed
10 White, SJ, Wright, CG, Robinson, KS, Meyerhoff, ML. Effect of topical hyaluronic acid on experimental cholesteatoma. Am J Otolaryngol 1995;16:312–18CrossRefGoogle ScholarPubMed
11 Pownell, PH, Wright, CG, Robinson, KS, Meyerhoff, WL. The effect of cyclophosphamide on development of experimental cholesteatoma. Arch Otolaryngol Head Neck Surg 1994;120:1114–16Google Scholar
12 Kayhan, FT, Algun, Z, The effect of naproxen sodium on experimental otitis media. Kulak Burun Bogaz Ihtis Derg 2008;18:14–18 Google Scholar
13 Yilmaz, M, Goksu, N, Bayramoglu, I, Bayazit, YA. Practical use of MESNA in atelectatic ears and adhesive otitis media. ORL J Otorhinolaryngol Relat Spec 2006;68:195–8Google Scholar
14 Kalcioglu, MT, Cicek, MT, Bayindir, T, Ozdamar, OI. Effectiveness of MESNA on the success of cholesteatoma surgery. Am J Otolaryngol 2014;35:357–61Google Scholar
15 Casale, M, Di Martino, A, Salvinelli, F, Trombetta, M, Denaro, V. MESNA for chemically assisted tissue dissection. Expert Opin Investig Drugs 2010;19:699–707 Google Scholar
16 Song, J, Liu, L, Li, L, Liu, J, Song, E, Song, Y. Protective effects of lipoic acid and mesna on cyclophosphamide-induced haemorrhagic cystitis in mice. Cell Biochem Funct 2014;32:125–32CrossRefGoogle ScholarPubMed
17 Vincenti, V, Mondain, M, Pasanisi, E, Piazza, F, Puel, JL, Bacciu, S, et al. Cochlear effects of Mesna application into middle ear. Ann N Y Acad Sci 1999;884:425–32CrossRefGoogle ScholarPubMed
18 Van Spaendonck, MP, Timmermans, JP, Claes, J, Scheuermann, W, Wuyts, FL, Van De Heyning, PH. Single ototopical application of mesna has no ototoxic effects on guinea pig cochlear hair cells: a morphological study. Acta Otolaryngol 1999;119:685–9Google Scholar
19 Vincenti, V, Magnan, J, Zini, C. Cochlear effects of intraoperative use of Mesna in cholesteatoma surgery. Acta Biomed 2014;85:30–4Google ScholarPubMed
20 Vincenti, V, Magnan, J, Saccardi, MS, Zini, C. Chemically assisted dissection by means of Mesna in cholesteatoma surgery. Otol Neurotol 2014;35:1819–24CrossRefGoogle ScholarPubMed
21 Jove, MA, Vassalli, L, Raslan, W, Aoolebaum, EL. The effect of isotretinoin on propylene glycol-induced cholesteatoma in chinchilla middle ears. Am J Otolaryngol 1990;11:5–9 CrossRefGoogle ScholarPubMed
22 Miodoński, AJ, Litwin, JA, Składzień, J, Zagórska-Świeży, K. The structure of acquired aural cholesteatoma as revealed by scanning electron microscopy. Folia Morphol (Warsz) 2008;67:8–12 Google Scholar
23 Maniu, A, Harabagiu, O, Schrepler, MP, Cătănă, A, Fănută, B, Mogoantă, CA. Molecular biology of cholesteatoma. Rom J Morphol Embryol 2014;55:7–13 Google Scholar
24 Louw, L. Acquired cholesteatoma pathogenesis: stepwise explanations. J Laryngol Otol 2010;124:587–93Google Scholar
25 Eskiizmir, G, Yuceturk, AV, Onur, E, Var, A, Temiz, P. The imbalance of enzymatic antioxidants in cholesteatoma. Acta Otolaryngol 2009;129:1187–91Google Scholar
26 Sudhoff, H, Tos, M. Pathogenesis of sinus cholesteatoma. Eur Arch Otorhinolaryngol 2007;264:1137–43CrossRefGoogle ScholarPubMed
27 Feng, X, Coulombe, PA. A role of disulfide bonding in keratin intermediate filaments organization and dynamics in skin keratinocytes. J Cell Biol 2015;209:59–72 Google Scholar