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Role of Langerhans cells, Ki-67 protein and apoptosis in acquired cholesteatoma: prospective clinical study

Published online by Cambridge University Press:  18 January 2013

V Akdogan ,
I Yilmaz ,
T Canpolat  and
L N Ozluoglu
V Akdogan
Affiliation:
Department of Otolaryngology, Baskent University School of Medicine, Ankara, Turkey
I Yilmaz*
Affiliation:
Department of Otolaryngology, Baskent University School of Medicine, Ankara, Turkey
T Canpolat
Affiliation:
Department of Pathology, Baskent University School of Medicine, Ankara, Turkey
L N Ozluoglu
Affiliation:
Department of Otolaryngology, Baskent University School of Medicine, Ankara, Turkey
*
Address for correspondence: Dr Ismail Yilmaz, Otolaryngology Department, Baskent University, Adana Seyhan Hospital, Baraj Yolu 1 durak No 37, 01110 Seyhan, Adana, Turkey Fax: +90 322 4599197 E-mail: [email protected]
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Abstract

Objective:

To investigate the role of Langerhans cells in the pathogenesis and clinical picture of middle-ear cholesteatoma.

Subjects and methods:

The study included 40 patients operated upon for a diagnosis of chronic otitis due to acquired cholesteatoma.

Results and analysis:

A closed surgical technique was used in 20 per cent of patients and an open technique in 80 per cent. Langerhans cells were more densely accumulated in cholesteatoma epithelium, compared with external ear canal skin (p < 0.001). Staining for Ki-67 protein was greater in cholesteatoma epithelium (p < 0.001) and Apo2.7 protein staining (indicating apoptosis) was more prominent (p < 0.001), compared with ear canal skin. Regarding significant relationships between clinical and pathological findings, staining for Ki-67 (p = 0.046) and Apo2.7 (p = 0.037) was more prominent in patients undergoing open versus closed surgery.

Conclusion:

Using cell proliferation and apoptosis markers, a dense Langerhans cell infiltration was found to occur as a host response to middle-ear cholesteatoma.

Keywords

Cell ProliferationCholesteatomaLangerhans CellsApoptosis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 3 , March 2013 , pp. 252 - 259
Copyright
Copyright © JLO (1984) Limited 2013

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Footnotes

Presented at the XXXth National Congress of Turkish Otorhinolaryngology and Head and Neck Surgery, 8–12 October 2008, Antalya, Turkey

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