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Quantitative analysis of the bacterial findings in otitis media

Published online by Cambridge University Press:  29 June 2007

Lars-Eric Stenfors*
Affiliation:
Depaments of Otolaryngology, University of Tromsö, Tromsö, Norway and Central Hospital of Keski-Pohjanmaa, Kokkola, Finland
Simo Räisänen
Affiliation:
Clinical Laboratory, Central Hospital of Keski-Pohjanmaa, Kokkola, Finland
*
Lars-Eric Stenfors, MD, Dept. of Otolaryngology, Central Hospital of Keski-Pohjanmaa, SF-67200 Kokkola, Finland

Abstract

Qualitative and quantitative bacterial analysis of 200 samples of middle ear effusions collected from patients with current otitis media was performed. When middle ear pathogens (S. pneumoniae, H. influenzae and B. catarrhalis) where found during current acute otitis media or otitis media with effusion infection, the quantity of these bacteria was of the magnitude 106–108/ml and 0–5 × 105/ml effusion material, respectively. Mucopurulent effusion material contained 6 × 105–108 bacteria per ml whereas effusion from chronically discharging ears exceeded 109 bacteria per ml. Serous effusions did not harbour middle ear pathogens. The appearance of the effusion material was dependent on the number of bacteria involved. Quantification of bacteria in various middle ear effusions offers opportunities to make the diagnosis of various otitis media infections more accurate and readily comparable.

Type
Research Article
Copyright
Copyright © JLO (1984) Limited 1990

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References

Brumfitt, W., Percival, A., Williams, J. D. (1973). Estimation of bacteria and cells in the urine. American Journal of Clinical Pathology, 8: 111.Google Scholar
Faden, H., Dryja, D. (1989). Recovery of aunique bacterial organism in human middle ear fluid and its possible rolein chronic otitis media. Journal of Clinical Microbiology, 27: 24882491.CrossRefGoogle Scholar
Hanes, C. V. E., Lucia, H. L. (1988). Acridine orange as a screen for organisms in clinical specimens and comparision with Gram's stain. Archives of Pathology and Laboratory Medicine, 112: 529532.Google Scholar
Jonsson, L., Schwan, A., Thomander, L., Fabian, P. (1986). Aerobic and anaerobic bacteria in chronic suppurative otitis media. A quantitative study. Acta Otolaryngologica, 102: 410414.Google Scholar
Kamme, C., Lundgren, K., Mårdh, P.-A. (1971). The aetiology of acute otitis media in children. Scandinavian Journal of Infectious Diseases, 3: 217223.Google Scholar
Karma, P., Jokipii, I., Ojala, K., Jokipii, A. M. M. (1978). Bacteriology of the chronically discharging middle ear. Acta Otolaryngologica, 86: 110114.CrossRefGoogle ScholarPubMed
Karma, P. H., Sipilä, P. T., Luotonen, J. P., Grönroos, W. P. (1986). Bacteriological aspects of acute otitis media and secretory otitis media. In Acute and Secretory Otitis Media(Sadé, J., ed), pp. 181188, Kugler Publication, Amsterdam/Berkeley.Google Scholar
Lim, D. J. (1980). Recent advances in otitis media with effusion. Annals of Otology, Rhinology and Laryngology, 89 (Supplement).Google Scholar
Lim, D. J. (1985). Recent advances in otitis mediawith effusion. Annals of Otology, Rhinology and Laryngology, 94 (Supplement 116): 89.Google Scholar
Lim, D. J. (1989). Recent advances in otitis media. Annals of Rhinology and Laryngology, 98 (Supplement 139): 10.Google Scholar
Parsons, C. L., Mulholland, S. G., Anwar, H. (1979). Antibacterial activity of bladder surface mucin duplicated by exogenous glycosaminoglucan (heparin). Infection and Immunity, 24:552557.Google Scholar
Sarkkinen, H., Ruuskanen, O., Meurman, O., Puhakka, H., Virolainen, E., Eskola, J. (1985). Identification of respiratory Virus antigens in middle ear fluidsof children with otitis media. Journal of Infectious Diseases, 151: 444448.CrossRefGoogle ScholarPubMed
Stenfors, L.-E., Räisänen, S. (1988). Quantification of bacteria middle ear effusions. Acta Otolaryngologica, 106: 435440.Google Scholar
Stenfors, L.-E., Räisänen, S. (1989). How long do middle pathogens survive in mucoid effusion material? Acta Otolaryngica, 107: 244248.CrossRefGoogle Scholar
Van Cauwenberge, P. (1982). Otitis media with effusion. Acta Otorhino-laryngologica Belgica, 36: 196207.Google Scholar