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The predictive value of p53, MDM-2, cyclin D1 and Ki67 in the progression from low-grade dysplasia towards carcinoma of the larynx

Published online by Cambridge University Press:  29 June 2007

Lorenzo Pignataro ,
Pasquale Capaccio ,
Giancarlo Pruneri ,
Nadia Carboni ,
Roberto Buffa ,
Antonino Neri  and
Antonio Ottaviani
Lorenzo Pignataro*
Affiliation:
Clinica Otorinolaringoiatrica, Servizio di Ematologia, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore di Milano, I.R.C.C.S., 20122, Milan, Italy
Pasquale Capaccio
Affiliation:
Clinica Otorinolaringoiatrica, Servizio di Ematologia, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore di Milano, I.R.C.C.S., 20122, Milan, Italy
Giancarlo Pruneri
Affiliation:
II Servizio di Anatomia Patologica, Servizio di Ematologia, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore di Milano, I.R.C.C.S., 20122, Milan, Italy
Nadia Carboni
Affiliation:
II Servizio di Anatomia Patologica, Servizio di Ematologia, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore di Milano, I.R.C.C.S., 20122, Milan, Italy
Roberto Buffa
Affiliation:
II Servizio di Anatomia Patologica, Servizio di Ematologia, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore di Milano, I.R.C.C.S., 20122, Milan, Italy
Antonino Neri
Affiliation:
Laboratorio di Ematologia Sperimentale e Genetica Molecolare, Servizio di Ematologia, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore di Milano, I.R.C.C.S., 20122, Milan, Italy
Antonio Ottaviani
Affiliation:
Clinica Otorinolaringoiatrica, Servizio di Ematologia, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore di Milano, I.R.C.C.S., 20122, Milan, Italy
*
Address for correspondence: Lorenzo Pignataro, M.D., Istituto di Clinica Otorhinolaringoiatrica IOspedale Maggiore, I.R.C.C.S. Via F. Sforza, 35 20122 Milano, Italy. Fax 0039 2 55180809
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Abstract

To evaluate the predictive role of the oncogenes p53, MDM-2 and cyclin D1, and the proliferative marker Ki67, in the progression from low-grade dysplasia to carcinoma of the larynx. We studied immunohistochemically a series of 32 low-grade pre-neoplastic laryngeal lesions, 10 of which progressed to invasive carcinoma. Immunoreactivity in more than 10 per cent of the dysplastic cells was detected in five cases immunostained with anti-p53 (≈ 15 per cent), in two with anti-MDM-2 (≈ six per cent), and 11 with anti-Ki67 antibodies (≈ 34 per cent), whereas none of the cases showed cyclin D1 overexpression. No significant association was found between p53 and MDM-2 immunoreactivity and the evolution to carcinoma; on the contrary, Ki67 expression was detectable in all but one of the 10 cases developing an infiltrative tumour (90 per cent), and in two of the 22 cases that did not progress (≈ nine per cent) (p = 0.01). These findings indicate that immunohistochemical assessment of the proliferative index in bioptic samples of dysplastic laryngeal mucosa may be useful in selecting patients who should undergo a more specific follow-up evaluation.

Keywords

OncogenesTumour markers, biologicalInununohistochemistrylaryngeal neoplasms
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 112 , Issue 5 , May 1998 , pp. 455 - 459
Copyright
Copyright © JLO (1984) Limited 1998

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Footnotes

Presented in part at the 20th National Congress of the Italian Society of Surgical Oncology, Rome, Italy, October 17–18, 1996.

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