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Pomegranate extract: a potential protector against aminoglycoside ototoxicity

Published online by Cambridge University Press:  22 January 2014

V Kahya ,
B Ozucer ,
R Dogan ,
A Meric ,
M Yuksel ,
O Gedikli  and
O Ozturan
V Kahya
Affiliation:
Otorhinolaryngology Department, Medical Faculty, Bezmialem Vakif University, Istanbul, Turkey
B Ozucer*
Affiliation:
Otorhinolaryngology Department, Medical Faculty, Bezmialem Vakif University, Istanbul, Turkey
R Dogan
Affiliation:
Department of Otolaryngology, Bayrampasa State Hospital, Istanbul, Turkey
A Meric
Affiliation:
Otorhinolaryngology Department, Medical Faculty, Bezmialem Vakif University, Istanbul, Turkey
M Yuksel
Affiliation:
Medical Laboratory Department, Vocational School of Health Related Professions, Marmara University, Istanbul, Turkey
O Gedikli
Affiliation:
Otolaryngology Department, School of Health Related Professions, Kirklareli University, Turkey
O Ozturan
Affiliation:
Otorhinolaryngology Department, Medical Faculty, Bezmialem Vakif University, Istanbul, Turkey
*
Address for correspondence: Dr Berke Ozucer, Department of Otorhinolaryngology, Medical Faculty, Bezmialem Vakif University, Fatih, Istanbul, Turkey Fax: +90 2125332326 E-mail: [email protected]
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Abstract

Objective:

To investigate the effectiveness of pomegranate extract as protection against aminoglycoside ototoxicity.

Design:

Prospective, randomised, controlled, experimental study.

Subjects:

Eighteen Wistar albino rats were randomly allocated to 5 days of either: saline injections; gentamicin injections; or pomegranate extract (100 µl/day via gavage) plus gentamicin injections. Distortion product otoacoustic emissions were tested before treatment and on day 3. After treatment, reactive oxygen species levels were measured in each rat's right cochlea and right kidney via chemiluminescence.

Results:

Baseline emission amplitudes were similar. Post-treatment emissions differed significantly in the two treatment groups (p < 0.001). Cochlear reactive oxygen species levels were significantly higher in the gentamicin group (mean ± standard deviation, 316.6 ± 36.5 relative light units per mg) than the gentamicin plus pomegranate extract group (240 ± 24.6 relative light units per mg) (p = 0.004); control group levels were 119.1 ± 10.3 relative light units per mg. Renal reactive oxygen species levels were similar for the control and gentamicin plus pomegranate extract groups (p = 0.59) but much higher in the gentamicin group (p = 0.004).

Conclusion:

Concurrent systemic pomegranate extract administration reduced reactive oxygen species level increases and otoacoustic emission changes, following aminoglycoside injection.

Keywords

GentamicinPunicaceaeRatsReactive Oxygen SpeciesAntioxidantsOtoacoustic Emissions, SpontaneousHearing Loss, Noise-Induced
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 128 , Issue 1 , January 2014 , pp. 43 - 48
Copyright
Copyright © JLO (1984) Limited 2014 

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