Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-23T12:32:02.128Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphological evidence of biofilm in chronic obstructive sialadenitis

Published online by Cambridge University Press:  10 July 2018

S A Schrøder ,
S Eickhardt ,
T Bjarnsholt ,
T Nørgaard  and
P Homøe
S A Schrøder*
Affiliation:
Department of Otorhinolaryngology, Nordsjaellands University Hospital, Hillerød, Denmark Department of Otorhinolaryngology Head and Neck Surgery, Rigshospitalet – Copenhagen University Hospital, University of Copenhagen, Denmark
S Eickhardt
Affiliation:
Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
T Bjarnsholt
Affiliation:
Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark Department of Clinical Microbiology, Copenhagen University Hospital, Denmark
T Nørgaard
Affiliation:
Department of Pathology, Rigshospitalet – Copenhagen University Hospital, University of Copenhagen, Denmark
P Homøe
Affiliation:
Department of Otorhinolaryngology and Maxillofacial Surgery, Zealand University Hospital, Køge, Denmark
*
Address for correspondence: Dr Stine A Schrøder, Department of Otorhinolaryngology, Nordsjaellands University Hospital, Hillerød, Denmark Fax: +45 4829 3811 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective

Bacterial infection is a common finding in acute sialadenitis and may play a role in the chronicity of the condition. This study investigated if bacterial biofilm is present in submandibular chronic obstructive sialadenitis.

Methods

A descriptive case–control study was conducted that compared 10 histological sections of submandibular glands with chronic obstructive sialadenitis, to 10 histological sections of the healthy part of submandibular glands with pleomorphic adenoma. Fluorescence in situ hybridisation and confocal laser scanning microscopy visualised evidence of bacterial biofilm.

Results

In the chronic obstructive sialadenitis group, 5 out of 10 histological sections showed morphological evidence of bacterial biofilm. In the control group, there was no sign of bacterial biofilm formation.

Conclusion

Morphological evidence of bacterial biofilm was found in the submandibular gland sections from patients with chronic sialadenitis and suggests a role in the chronicity of submandibular chronic obstructive sialadenitis.

Keywords

BacteriaBiofilmsSalivary Gland CalculiSialadenitis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 132 , Issue 7 , July 2018 , pp. 611 - 614
Copyright
Copyright © JLO (1984) Limited, 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Dr S A Schrøder takes responsibility for the integrity of the content of the paper

References

1Bradley, PJ. Benign salivary gland disease. Hosp Med 2001;62:392–5Google Scholar
2Seifert, G. Aetiological and histological classification of sialadenitis. Pathologica 1997;89:7–17Google Scholar
3Bradley, PJ. Microbiology and management of sialadenitis. Curr Infect Dis Rep 2002;4:217–24Google Scholar
4Marcus, RJ, Post, JC, Stoodley, P, Hall-Stoodley, L, McGill, RL, Sureshkumar, KK et al. Biofilms in nephrology. Expert Opin Biol Ther 2008;8:1159–66Google Scholar
5Parsek, MR, Singh, PK. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol 2003;57:677–701Google Scholar
6Homoe, P, Bjarnsholt, T, Wessman, M, Sorensen, HC, Johansen, HK. Morphological evidence of biofilm formation in Greenlanders with chronic suppurative otitis media. Eur Arch Otorhinolaryngol 2009;266:1533–8Google Scholar
7Teymoortash, A, Wollstein, AC, Lippert, BM, Peldszus, R, Werner, JA. Bacteria and pathogenesis of human salivary calculus. Acta Otolaryngol 2002;122:210–14Google Scholar
8Fusconi, M, Petrozza, V, Schippa, S, de Vincentiis, M, Familiari, G, Pantanella, F et al. Bacterial biofilm in salivary gland stones: cause or consequence? Otolaryngol Head Neck Surg 2016;154:449–53Google Scholar
9Bjarnsholt, T, Moser, C, Jensen, PØ, Højby, N. Biofilm Infections. London: Springer, 2011Google Scholar
10Aas, JA, Paster, BJ, Stokes, LN, Olsen, I, Dewhirst, FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 2005;43:5721–32Google Scholar
11Marchal, F, Kurt, AM, Dulguerov, P, Lehmann, W. Retrograde theory in sialolithiasis formation. Arch Otolaryngol Head Neck Surg 2001;127:66–8Google Scholar
12Koch, M, Zenk, J, Iro, H. Algorithms for treatment of salivary gland obstructions. Otolaryngol Clin North Am 2009;42:1173–92Google Scholar