Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-12-01T00:45:06.590Z Has data issue: false hasContentIssue false
  • English
  • Français

Long-term ototoxic effects of neomycin applied topically in the middle ear: A morphological study in the guinea pig

Published online by Cambridge University Press:  29 June 2007

Darí Morais ,
Miguel Gonz´lez ,
Rosario Del Villar  and
Manuel J. Gayoso
Darí Morais
Affiliation:
From the Department of Otorhinolaryngology and Histology, University of Valladolid (Spain).
Miguel Gonz´lez
Affiliation:
From the Department of Otorhinolaryngology and Histology, University of Valladolid (Spain).
Rosario Del Villar
Affiliation:
School of Medicine, University of Valladolied (Spain).
Manuel J. Gayoso*
Affiliation:
School of Medicine, University of Valladolied (Spain).
*
M. J. Gayoso, Departmento de Histologia, Facultad de Medicina, Avda, Ramón y Cajal no7, 47011 Valladolid, Span.
Get access Rights & Permissions [Opens in a new window]

Abstract

Neomycin was instilled daily, uni-or bilaterally, into the middle ear of guinea pigs for three months. The cochleae were examined, by light and electron microscopy, six months after the end of treatment. The organs of Corti of the treated ears were completely destroyed, and in the most advanced lesions, were substituted by a single layer of very thin flat cells. In the spiral ganglion only some glial cells and a few neurons could be observed. All surviving neurons were myelinated, and their ultrastructure was greatly altered, with disappeared, losing it amorphous and filamentous components. The spiral limbus and the stria vascularis were atrophic and were also covered, in the final stages, by flat elongated cells. In view of its morphological characteristics, this epithelium may arise from the displacement of the interdental cells and perhaps from the cochlear surface of Reissner's membrane.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 102 , Issue 4 , April 1988 , pp. 304 - 307
Copyright
Copyright © JLO (1984) Limited 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ballantyne, J. (1983) Ototoxicity. (Proceedings of the sixth British Academic Conference in Otolaryngology) Journal of Laryngology and Otology. Supplement 8: 9–12.Google ScholarPubMed
Brummett, R. E. (1980) Drug-induced ototoxicity. Drugs, 19: 412–428.CrossRefGoogle ScholarPubMed
Brummett, R. E., Harris, R. F. and Lindgren, J. A. (1976) Detection of ototoxicity from drugs applied topically to the middle ear space. Laryngoscope, 86: 1177–1187.CrossRefGoogle Scholar
Duvall, A. J. and Wersäll, J. (1964) Site of action streptomycin upon inner ear sensory cells. Ada Otolaryngologica, 57: 581–598.CrossRefGoogle ScholarPubMed
Engstrom, H. and Kohonen, A. (1965) Cochlear damage from ototoxic antibiotic. Ada Otolaryngologica, 59: 171–178.CrossRefGoogle Scholar
Fuller, A. (1960) Ototoxicity of neomycin aerosol. Lancet, 1: 1026.CrossRefGoogle Scholar
Gibson, W. S. (1967) Deafness due to orally administered neomycin. Archives of Otolarvngology, 86: 163–165.CrossRefGoogle ScholarPubMed
Ginsberg, T. A., Rudnick, M. D. and Huber, P. S. (1980) Aminoglycoside ototoxicity following middle ear injection. III. Comparative quantitative analysis. Annals of Otology, Rhinology and Laryngology, 89, Supplement 77: 17–24.CrossRefGoogle Scholar
Gonzalez, M. and Ona, J. (1975) Algunosaspectoshistoquimicosde la laberintotoxia kanamicinica. Ada Otorrinolaringologica Espanola, 26: 125–134.Google Scholar
Ilberg, C. V., Spoendlin, H. and Arnold, W. (1971) Autorradiographical distribution of locally applied dihydrostreptomycin in the inner ear. Ada Otolaryngologica, 71: 159–165.CrossRefGoogle Scholar
Kacker, S. K. (1970) Neomycin-induced spiral organ degeneration in the guinea pig. Laryngoscope, 80: 391–399.CrossRefGoogle ScholarPubMed
Kaku, Y, Farmer, C. and Hudson, R. (1973) Ototoxic drug effects on cochlear histochemistry. Archives of Otolarvngology, 98: 282–286.CrossRefGoogle ScholarPubMed
Keene, M and Hawke, M. (1981) The pathogenesis and detention of aminoglycoside ototoxicity. Journal of Otolaryngology, 10: 228–236.Google Scholar
Kelly, D. R., Nilo, E. R. and Berggren, R. B. (1969) Deafness after topical neomycin wound irrigation. New England Journal of Medicine, 280: 1338–1339.CrossRefGoogle ScholarPubMed
Kohonen, A. and Tarkkanen, J. (1969) Cochlear damage from ototoxic antibiotics by intratympanic application. Ada Otolaryngologica, 68: 90–97.CrossRefGoogle ScholarPubMed
Koide, Y., Hata, A. and Hando, R. (1966) Vulnerability of the organ of Corti in poisoning. Ada Otolaryngologica, 61: 332–344.CrossRefGoogle ScholarPubMed
Mittelman, H. (1972) Ototoxicity of “ototopical” antibiotics: post, present and future. Transactions of the American Academy of Ophthalmology and Otolaryngology, 76: 1432–1443.Google Scholar
Prentki, M., Deeney, J. T., Matschinsky, F. M. and Joseph, S. K. (1986) Neomycin: a specific drug to study the inositolphos-pholipid signalling system? Febs Letters, 197: 285–288.CrossRefGoogle Scholar
Price, D. L. and Porter, K. R. (1972) The response of ventral horn neurons to axonal transection. Journal of Cell Biology, 53: 24–37.CrossRefGoogle ScholarPubMed
Rudnick, M. D., Guinsberg, I. A., Huber, P. S. and Rose, J. S. (1980) Aminoglycoside ototoxicity following middle ear injection. Annals of Otology, Rhinology and Laryngology, 89, Supplement 77: 10–16.CrossRefGoogle ScholarPubMed
Schuknecht, H. F. (1976) Pathophysiology. In: Pathology of the ear, 2nd edn, pp. 97–163, Harward University Press, Cambridge, Massachusetts.Google Scholar
Spoendlin, H. (1966) Zur Ototoxizitat des Streptomycins. Practica Otorhinolaryngologica, 28: 305–322.Google Scholar
Spoendlin, H. (1974) Neuroanatomy of the cochlea. In: Electrical Stimulation of the Acoustic Nerve in Man. Merzenich, M. M., Schindler, R. A. and Sooy, F. A. eds, pp. 7–23. Velo-Bind, Inc. San Francisco.Google Scholar
Spurr, A. R. (1969) A low viscosity epoxiresin embedding medium for electron microscopy. Journal of Ultrastrudure Research, 26: 31–43.CrossRefGoogle ScholarPubMed
Tachibana, M., Anniko, M. and Schacht, J. (1983) Effects of per-ilymphatically perfused gentamicin on microphonic potential, lipid labelling and morphology of cochlear tissues. Acta Otolaryngologica, 96: 31–38.CrossRefGoogle ScholarPubMed
Tai, P. C., Wallace, B. J. and Davis, B. D. (1978) Streptomycin causes misreading of natural messenger by interesting with ribosomes after initiation. Proceedings of National Academy of Sciences, 75: 275–279.CrossRefGoogle Scholar
Tanaka, K. and Motomura, S. (1981) Permeability of the labyrinthine windows in guinea pigs. Archives of Otorhinolaryngology, 233: 67–75.CrossRefGoogle ScholarPubMed