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Inhibitory effect of HuR gene small interfering RNA segment on laryngeal carcinoma Hep-2 cell growth

Published online by Cambridge University Press:  02 June 2010

Z Shen ,
D Ye ,
X Zhang ,
Z Jiang ,
B Xiao  and
J Guo
Z Shen*
Affiliation:
Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, China Institute of Biochemistry and Molecular Biology, Ningbo University School of Medicine, China
D Ye
Affiliation:
Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, China Institute of Biochemistry and Molecular Biology, Ningbo University School of Medicine, China
X Zhang
Affiliation:
Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, China Institute of Biochemistry and Molecular Biology, Ningbo University School of Medicine, China
Z Jiang
Affiliation:
Institute of Biochemistry and Molecular Biology, Ningbo University School of Medicine, China
B Xiao
Affiliation:
Institute of Biochemistry and Molecular Biology, Ningbo University School of Medicine, China
J Guo
Affiliation:
Institute of Biochemistry and Molecular Biology, Ningbo University School of Medicine, China
*
Address for correspondence: Zhisen Shen, M.D., Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, 315000, China. Tel.: +86-13906687216 Fax: +86-574-87392232 E-mail: [email protected]
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Abstract

Objectives:

To investigate the effect of the HuR gene on laryngeal carcinoma Hep-2 cell growth, and to analyse correlations between the HuR, cyclooxygenase-2 and survivin genes.

Study design:

Experiment study.

Setting:

Department of Otolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, a tertiary care centre in China.

Methods:

Copies of a small interfering RNA segment directed against the HuR gene were transfected into Hep-2 cells using LipofectamineTM 2000. The effect of the small interfering RNA segment on Hep-2 cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Changes in the expression of the HuR, cyclooxygenase-2 and survivin genes were detected by semi-quantitative reverse transcription polymerase chain reaction analysis. Concentrations of the HuR, cyclooxygenase-2 and survivin proteins were evaluated using Western blotting.

Results:

Expression of the HuR, cyclooxygenase-2 and survivin genes, as indicated by messenger RNA and protein levels, was suppressed by the HuR gene small interfering RNA segment in a dose-dependent manner. The proliferation indices of all treated groups were significanlty lower than those of control groups (p < 0.05).

Conclusions:

Impairment of HuR gene expression, using interfering RNA technology, can significantly suppress Hep-2 cell proliferation and induce apoptosis. The HuR gene may be an effective target for gene therapy in patients with laryngeal carcinoma.

Keywords

Laryngeal NeoplasmsCarcinomaSurvivin ProteinHuman GeneHuR Gene
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 11 , November 2010 , pp. 1183 - 1189
Copyright
Copyright © JLO (1984) Limited 2010

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