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Histological analysis of the effects of anti-adhesive haemostatic agents on the middle ear of the guinea pig

Published online by Cambridge University Press:  29 September 2014

E Kulduk ,
E Eren ,
FK Soy ,
R Dundar ,
M Aslan ,
MS Basoglu ,
F Simsek  and
H Katilmis
E Kulduk*
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
E Eren
Affiliation:
Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
FK Soy
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
R Dundar
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
M Aslan
Affiliation:
Department of Otorhinolaryngology, Mardin State Hospital, Turkey
MS Basoglu
Affiliation:
Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
F Simsek
Affiliation:
Department of Histology and Embryology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
H Katilmis
Affiliation:
Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Turkey
*
Address for correspondence: Dr Erkan Kulduk, Department of Otolaryngology, Mardin State Hospital, Mardin, Turkey Fax: +90 232 243 1530 E-mail: [email protected]
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Abstract

Introduction:

Adhesion formation is a frequent and serious post-operative problem in ENT surgery. This study assessed the effect of two anti-adhesive haemostatic agents on an experimental guinea pig model.

Materials and methods:

The middle-ear mucosa of 14 guinea pigs was exposed to surgical trauma. After surgery, Arista™ AH was injected into the right middle ear of seven animals, while Ankaferd Blood Stopper was injected into the right middle ear of the other seven animals. The left ears were left untreated and regarded as the control group. The three groups were compared by histological examination at post-operative week 4.

Results:

In each of the three groups, consolidation of the lamina propria and epithelium mucosae, increments in the number of active fibroblasts, collagen fibrils and inflammatory cells, and increased vascular dilation were observed on haematoxylin and eosin-stained sections, and were more prominent in the control and Ankaferd Blood Stopper groups. Epithelial thickness and capillary vasodilation were significantly lower in the Arista™ AH group compared with the control and Ankaferd Blood Stopper groups (p < 0.008).

Conclusion:

Arista™ AH may prevent the formation of adhesions in middle-ear surgery. Further experimental studies are required to determine its ototoxic potential.

Keywords

Guinea PigsEar, MiddleSurgery-Induced Tissue Adhesions
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 128 , Issue 10 , October 2014 , pp. 885 - 891
Copyright
Copyright © JLO (1984) Limited 2014 

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