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High-speed video analysis of acoustically oscillated guinea pig stapes

Published online by Cambridge University Press:  23 February 2015

K Ishizu ,
T Yasui ,
M Ohashi ,
N Matsumoto  and
S Komune
K Ishizu
Affiliation:
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
T Yasui
Affiliation:
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
M Ohashi
Affiliation:
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
N Matsumoto*
Affiliation:
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
S Komune
Affiliation:
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
*
Address for correspondence: N Matsumoto, Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan Fax:+81 92 642 5685, Phone: +81 92 642 5668, E-mail: [email protected]
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Abstract

Objective:

We investigated the ossicular movement in the near-intact middle ear in response to acoustic stimulation using a high-speed video camera and video analysis software program.

Design:

We have designed a good visual access to the middle ear of the guinea pig by opening the ventral wall of the otic capsule, without injuring the sound-conducting structures, from the external auditory canal to the oval window. The high-speed video camera could record analysable ossicular motion up to 4000 frames per second.

Results:

The stapes showed reciprocal movement in the same frequency as the stimulating tone, and with an amplitude proportional to the stimulating sound intensity. Injury to the tympanic membrane attenuated the stapedial motion, which was recovered to that of the control level by patch repair of the perforation.

Conclusion:

Our experimental set-up was capable of evaluating the conductive hearing, regardless of the status of the animal's sensorineural hearing or even life. Such a video analysis may provide a powerful tool to investigate the physiology of the middle ear.

Keywords

High-speed CameraEar OssiclesEar MiddleBone Conduction
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 129 , Issue S2 , March 2015 , pp. S33 - S37
Copyright
Copyright © JLO (1984) Limited 2015 

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References

1Goode, RL, Ball, G, Nishihara, S. Measurement of umbo vibration in human subjects – method and possible clinical applications. Am J Otol 1993;14:247–51Google Scholar
2Goode, RL, Ball, G, Nishihara, S, Nakamura, K. Laser Doppler vibrometer (LDV) – a new clinical tool for the otologist. Am J Otol 1996;17:813–22Google Scholar
3Sim, JH, Lauxmann, M, Chatzimichalis, M, Roosli, C, Eiber, A, Huber, AM. Errors in measurement of three-dimensional motions of the stapes using a laser Doppler vibrometer system. Hear Res 2010;270:4–14CrossRefGoogle ScholarPubMed
4Sim, JH, Chatzimichalis, M, Lauxmann, M, Roosli, C, Eiber, A, Huber, AM. Complex stapes motions in human ears. J Assoc Res Otolaryngol 2010;11:329–41CrossRefGoogle ScholarPubMed
5Hato, N, Stenfelt, S, Goode, RL. Three-dimensional stapes footplate motion in human temporal bones. Audiol Neurootol 2003;8:140–52Google Scholar
6Rosowski, JJ, Nakajima, HH, Merchant, SN. Clinical utility of laser-Doppler vibrometer measurements in live normal and pathologic human ears. Ear Hear 2008;29:3–19Google Scholar
7Huber, AM, Schwab, C, Linder, T, Stoeckli, SJ, Ferrazzini, M, Dillier, N et al. Evaluation of eardrum laser Doppler interferometry as a diagnostic tool. Laryngoscope 2001;111:501–7CrossRefGoogle ScholarPubMed
8Nakajima, HH, Pisano, DV, Roosli, C, Hamade, MA, Merchant, GR, Mahfoud, L et al. Comparison of ear-canal reflectance and umbo velocity in patients with conductive hearing loss: a preliminary study. Ear Hear 2012;33:35–43Google Scholar
9Chien, W, Rosowski, JJ, Merchant, SN. Investigation of the mechanics of Type III stapes columella tympanoplasty using laser-Doppler vibrometry. Otol Neurotol 2007;28:782–7CrossRefGoogle ScholarPubMed
10Huber, A, Linder, T, Ferrazzini, M, Schmid, S, Dillier, N, Stoeckli, S et al. Intraoperative assessment of stapes movement. Ann Otol Rhinol Laryngol 2001;110:31–5Google Scholar
11Gan, RZ, Cheng, T, Dai, C, Yang, F, Wood, MW. Finite element modeling of sound transmission with perforations of tympanic membrane. J Acoust Soc Am 2009;126:243–53CrossRefGoogle ScholarPubMed
12Sun, Q, Chang, KH, Dormer, KJ, Dyer, RK Jr, Gan, RZ. An advanced computer-aided geometric modeling and fabrication method for human middle ear. Med Eng Phys 2002;24:595–606Google Scholar
13Gyo, K, Aritomo, H, Goode, RL. Measurement of the ossicular vibration ratio in human temporal bones by use of a video measuring system. Acta Otolaryngol 1987;103:87–95CrossRefGoogle ScholarPubMed
14Kitani, R, Kakehata, S, Kalinec, F. Motile responses of cochlear outer hair cells stimulated with an alternating electrical field. Hear Res 2011;280:209–18CrossRefGoogle ScholarPubMed
15Matsumoto, Y, Nomoto, T, Cabral, H, Matsumoto, Y, Watanabe, S, Christie, RJ et al. Direct and instantaneous observation of intravenously injected substances using intravital confocal micro-videography. Biomed Opt Express 2010;1:1209–16CrossRefGoogle ScholarPubMed
16Iijima, A, Minamitani, H, Ishikawa, N. Image analysis of quick phase eye movements in nystagmus with high-speed video system. Med Biol Eng Comput 2001;39:2–7Google Scholar
17Baloh, RW, Furman, JM. Modern vestibular function testing. West J Med 1989;150:59–67Google Scholar
18Saliba, I, Abela, A, Arcand, P. Tympanic membrane perforation: size, site and hearing evaluation. Int J Pediatr Otorhinolaryngol 2011;75:527–31Google Scholar
19Chien, W, Ravicz, ME, Merchant, SN, Rosowski, JJ. The effect of methodological differences in the measurement of stapes motion in live and cadaver ears. Audiol Neurootol 2006;11:183–97Google Scholar