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Genetic polymorphisms affecting antioxidant enzymes are present in tympanosclerosis patients

Published online by Cambridge University Press:  18 August 2016

A Akyigit ,
Ş Yalcin ,
E Ö Etem ,
İ Kaygusuz ,
T Karlidag ,
E Keles ,
Z B Celik  and
A Tektemur
A Akyigit*
Affiliation:
ENT Clinic, Elazığ Education and Research Hospital
Ş Yalcin
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
E Ö Etem
Affiliation:
Department of Medical Biology, Firat University Faculty of Medicine, Elazığ, Turkey
İ Kaygusuz
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
T Karlidag
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
E Keles
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
Z B Celik
Affiliation:
Department of Medical Biology, Firat University Faculty of Medicine, Elazığ, Turkey
A Tektemur
Affiliation:
Department of Medical Biology, Firat University Faculty of Medicine, Elazığ, Turkey
*
Address for correspondence: Dr A Akyigit, Elazığ Education and Research Hospital, 23100 Elaziğ, Turkey E-mail: [email protected]
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Abstract

Background:

This study investigated genetic polymorphisms affecting the inducible nitric oxide synthase, superoxide dismutase and catalase enzymes in chronic otitis media patients with and without tympanosclerosis, and the role of genetic susceptibility in the disease aetiology.

Methods:

A total of 162 patients who underwent surgery for chronic otitis media were divided into two study groups: a tympanosclerosis group and a chronic otitis media group. A third, the control, group comprised 188 healthy volunteers. Venous blood samples were evaluated using reverse transcriptase polymerase chain reaction.

Results:

There was a significant difference in GG genotype distribution of the −277A>G polymorphism in the NOS2 gene between the tympanosclerosis and control groups (p < 0.05). However, there were no significant between-group differences in the CC genotype distribution of the p.Ala16Val (C>T) polymorphism in the SOD2 gene (p > 0.05). There were significant differences in the TT genotype distribution of the −21A>T polymorphism in the CAT gene between the tympanosclerosis and control groups, and between the chronic otitis media and control groups (p < 0.05).

Conclusion:

These results suggest that genetic predisposition may play a role in the aetiopathogenesis of tympanosclerosis.

Keywords

Tympanosclerosis; Nitric Oxide Synthase Type II; Superoxide Dismutase; Catalase; PolymorphismGenetic
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 10 , October 2016 , pp. 928 - 933
Copyright
Copyright © JLO (1984) Limited 2016 

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