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Expression of transforming growth factor-α (TGF-α) in cholesteatoma

Published online by Cambridge University Press:  29 June 2007

Masanori Shiwa ,
Hiromi Kojima  and
Hiroshi Moriyama
Masanori Shiwa*
Affiliation:
Department of Otorhinolaryngology, The Jikei University School of Medicine, 3-25-8 Nishishinbashi Minato-ku, Tokyo 105-0003, Japan.
Hiromi Kojima
Affiliation:
Department of Otorhinolaryngology, The Jikei University School of Medicine, 3-25-8 Nishishinbashi Minato-ku, Tokyo 105-0003, Japan.
Hiroshi Moriyama
Affiliation:
Department of Otorhinolaryngology, The Jikei University School of Medicine, 3-25-8 Nishishinbashi Minato-ku, Tokyo 105-0003, Japan.
*
Address for correspondence: Dr Masanori Shiwa, Department of Otorhinolaryngology, The Jikei University School of Medicine, 3-25-8 Nishishinbashi Minato-ku, Tokyo 105-0003, Japan. Fax: +81-3-3435-8436
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Abstract

This study aims at elucidating the role of cytokines in the mechanism of proliferation of cholesteatoma epithelium by investigating the mode of expression of epidermal growth factors, such as TGF-α. The subjects of this study were patients who had undergone operation for middle ear cholesteatoma. Skins of the bone region of the externalear canal (normal skin) of the same patients were used as the negative control. The mode of expression of TGF-α was studied by immunohistochemistry and in situ hybridization. In the immunohistochemical study, there were no conspicuous differences observed between cholesteatoma tissues and normal skin. After in situ hybridization, expression of TGF-α mRNA was mainly observed in the epidermal basal cell layer in the normal skin, while in the cholesteatoma epidermis with severe inflammatory cell infiltration, expression of TFG-α mRNA was observed up to layers superior to the basal cell layer. The expression of TGF-α mRNA is greatly affected by subepithelial connective tissue, strongly suggesting involvement of paracrine regulation in proliferation of cholesteatomaepithelium.

Keywords

CholesteatomaCytokinesTGF-alpha
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 112 , Issue 8 , August 1998 , pp. 750 - 754
Copyright
Copyright © JLO (1984) Limited 1998

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