Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-09T15:32:17.608Z Has data issue: false hasContentIssue false

Expression of a disintegrin and metalloproteinase-33 protein in vocal fold polyps

Published online by Cambridge University Press:  08 July 2015

M V Akdogan*
Affiliation:
Department of Otolaryngology, Baskent University Faculty of Medicine, Ankara, Turkey
H Erinanc
Affiliation:
Department of Pathology, Baskent University Faculty of Medicine, Ankara, Turkey
O Topal
Affiliation:
Department of Otolaryngology, Baskent University Faculty of Medicine, Ankara, Turkey
S S Erbek
Affiliation:
Department of Otolaryngology, Baskent University Faculty of Medicine, Ankara, Turkey
*
Address for correspondence: Dr M V Akdogan, Department of Otorhinolaryngology, Baskent University Konya Research and Teaching Center, Saray Caddesi No. 1 Selcuklu, Konya 42080, Turkey Fax: +90 332 2570637 E-mail: [email protected]

Abstract

Background:

This study aimed to evaluate the association of a disintegrin and metalloproteinase-33 protein (‘ADAM-33’) expression in vocal polyp formation and to determine its correlation with clinical characteristics.

Methods:

Medical charts and histological sections of 32 patients diagnosed with vocal polyps who underwent surgery were analysed. Controls were histopathologically normal vocal fold tissues obtained from 36 patients who underwent surgery for laryngeal squamous cell carcinoma. Immunohistochemical staining was performed to detect ADAM-33 expression in epithelial cells, stroma and vessels.

Results:

All epithelial, stromal and vascular staining scores were significantly greater in polyp tissue than in controls (p < 0.001). Stromal ADAM-33 staining scores were higher in vocal polyp patients with a symptom duration of less than six months (p < 0.05). Vocal overuse or the presence of reflux symptoms, sinonasal symptoms or allergy did not affect ADAM-33 immunostaining scores (p = 0.05).

Conclusion:

In this study, ADAM-33 immunostaining was significantly increased in vocal polyps. Therefore, over-expression of this protein may be associated with vocal polyp pathogenesis.

Type
Main Articles
Copyright
Copyright © JLO (1984) Limited 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Martins, RH, Defaveri, J, Domingues, MA, de Albuquerque e Silva, R. Vocal polyps: clinical, morphological, and immunohistochemical aspects. J Voice 2011;25:98106CrossRefGoogle ScholarPubMed
2Altman, KW. Vocal fold masses. Otolaryngol Clin North Am 2007;40:1091–108CrossRefGoogle ScholarPubMed
3Johns, MM. Update on the etiology, diagnosis, and treatment of vocal fold nodules, polyps, and cysts. Curr Opin Otolaryngol Head Neck Surg 2003;11:456–61CrossRefGoogle ScholarPubMed
4Cipriani, NA, Martin, DE, Corey, JP, Portugal, L, Caballero, N, Lester, R et al. The clinicopathologic spectrum of benign mass lesions of the vocal fold due to vocal abuse. Int J Surg Pathol 2011;19:583–7CrossRefGoogle ScholarPubMed
5Edwards, DR, Handsley, MM, Pennington, CJ. The ADAM metalloproteinases. Mol Aspects Med 2008;29:258–89CrossRefGoogle ScholarPubMed
6Erbek, SS, Erinanc, H, Erbek, S, Topal, O, Kiyici, H. Expression of a disintegrin and metalloproteinase 33 protein in nasal polyposis: an immunohistochemical study. Am J Rhinol Allergy 2010;24:7982CrossRefGoogle ScholarPubMed
7Lee, JY, Park, SW, Chang, HK, Kim, HY, Rhim, T, Lee, JH et al. A disintegrin and metalloproteinase 33 protein in patients with asthma: Relevance to airflow limitation. Am J Respir Crit Care Med 2006;173:729–35CrossRefGoogle ScholarPubMed
8Puxeddu, I, Pang, YY, Harvey, A, Haitchi, HM, Nicholas, B, Yoshisue, H et al. The soluble form of a disintegrin and metalloprotease 33 promotes angiogenesis: implications for airway remodeling in asthma. J Allergy Clin Immunol 2008;121:1400–6CrossRefGoogle ScholarPubMed
9Wolfsberg, TG, Primakoff, P, Myles, DG, White, JM. ADAM, a novel family of membrane proteins containing A Disintegrin And Metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions. J Cell Biol 1995;131:275–8CrossRefGoogle Scholar
10Paulissen, G, Rocks, N, Gueders, MM, Crahay, C, Quesada-Calvo, F, Bekaert, S et al. Role of ADAM and ADAMTS metalloproteinases in airway diseases. Respir Res 2009;10:127CrossRefGoogle ScholarPubMed
11Topal, O, Erinanc, H, Ozer, C, Canpolat, ET, Celik, SB, Erbek, SS. Expression of “a disintegrin and metalloproteinase-33” (ADAM-33) protein in laryngeal squamous cell carcinoma. J Laryngol Otol 2012;126:511–15CrossRefGoogle Scholar
12van Goor, H, Melenhorst, WB, Turner, AJ, Holgate, ST. Adamalysins in biology and disease. J Pathol 2009;219:277–86CrossRefGoogle ScholarPubMed
13Dijkstra, A, Postma, DS, Noordhoek, JA, Lodewijk, ME, Kauffman, HF, ten Hacken, NH, Timens, W. Expression of ADAMs (“a disintegrin and metalloprotease”) in the human lung. Virchows Arch 2009;454:441–9CrossRefGoogle Scholar
14Garlisi, CG, Zou, J, Devito, KE, Tian, F, Zhu, FX, Liu, J et al. Human ADAM33: protein maturation and localization. Biochem Biophys Res Commun 2003;301:3543CrossRefGoogle ScholarPubMed
15Shaffiq, A, Haitchi, HM, Pang, YY, Alangari, AA, Jones, MG, Marshall, BG et al. A disintegrin and metalloprotease (ADAM) 33 protein in patients with pulmonary sarcoidosis. Respirology 2012;17:342–9CrossRefGoogle ScholarPubMed
16Karahan, N, Baspinar, S, Yariktas, M, Kapucuoglu, N. Matrix Metalloproteinases (MMP-2 and MMP-9) and cyclooxygenase-2 (COX-2) expressions in vocal fold polyps. J Voice 2009;23:2933CrossRefGoogle ScholarPubMed
17Duflo, SM, Thibeault, SL, Li, W, Smith, ME, Schade, G, Hess, MM. Differential gene expression profiling of vocal fold polyps and Reinke's edema by complementary DNA microarray. Ann Otol Rhinol Laryngol 2006;115:703–14CrossRefGoogle ScholarPubMed