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Effect of acoustic overstimulation on the glycocalyx and the ciliary interconnections in the organ of Corti: High resolution scanning electron microscopic investigation

Published online by Cambridge University Press:  29 June 2007

M. Takumida ,
L. Fredelius ,
D. Bagger-Sjöbäck ,
Y. Harada  and
J. Wersäll
M. Takumida*
Affiliation:
Department of Otolaryngology, Hiroshima University School of Medicine, Hiroshima, Japan
L. Fredelius
Affiliation:
Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden
D. Bagger-Sjöbäck
Affiliation:
Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden
Y. Harada
Affiliation:
Department of Otolaryngology, Hiroshima University School of Medicine, Hiroshima, Japan
J. Wersäll
Affiliation:
Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden
*
M. Takumida, Department of Otolaryngology, Hiroshima University School of Medicine, 1-2.3 Kasumicho, Minamiku, Hiroshima 734, Japan
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Abstract

Changes in ciliary interconnections in the organ of Corti are described after acoustic overstimulation using a special high resolution scanning electron microscope and tannic acid-osmium staining technique, giving an almost three dimensional view. Guinea pigs were exposed to a 3.85 kHz pure tone at an intensity of 120 dB for 22.5 minutes. The first detectable change was a disarrangement of the cilia with a loosening of the interconnections. The ciliary plasma membrane presented with an abnormally smooth appearance. The tip links connecting the tips of the stereocilia to their taller neighbours were also affected showing elongation or even disappearance. The fine granules which cover the tips of the tallest stereocilia of the outer hair cells were decreased. These findings suggest that acoustic overstimulation may affect the carbohydrate metabolism exceding to degeneration of ciliary interconnections resulting in a disarrangement and detachment of cilia. The tip links, which may participate in sensory cell transduction, seem also to be affected by acoustic overstimulation.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 103 , Issue 12 , December 1989 , pp. 1125 - 1129
Copyright
Copyright © JLO (1984) Limited 1989

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References

Albers, F. W. J., De Groot, J. C. M. J., Veldman, J. E. and Huizing, E. H. (1987) Effects of endolymphatic sac obliteration on the cochlear duct glycocalyx. Journal of Oto-Rhino-Laryngology and Its Related Specialties. 49: 277–281.CrossRefGoogle ScholarPubMed
Bagger-Sjöbäck, D. and Takumida, M. (1988) Geometrical array of the vestibular sensory hair bundle. Acta Otolaryngologica 106: 393–403.CrossRefGoogle ScholarPubMed
Flock, Å., Flock, B. and Murray, E. (1977) Studies on the sensory hairs of receptor cells in the inner ear. Acta Otolaryngologica. 83: 85–91.CrossRefGoogle ScholarPubMed
Fredelius, L. (1988) Degeneration pattern in the guinea pig organ of Corti after pure tone overstimulation. Thesis, Stockholm.Google Scholar
Furness, D. N. and Hackney, C. M. (1985) Cross-links between stereocilia in the guinea pig cochlea. Hearing Research 18: 177–188.CrossRefGoogle ScholarPubMed
Furness, D. N. and Hackney, C. M. (1986) High-resolution scanning-electron microscopy of stereocilia using the osmium-thio-carbohydrazide coating technique. Hearing Research. 21: 243–249.CrossRefGoogle ScholarPubMed
Hunter-Duval, I. M., Suzuki, M. and Mount, R. J. (1982) Anatomical changes in the organ of Corti after acoustic stimulation. In: New perspection in noise-induced hearing loss, Hamernik, R., Henderson, D. and Saloi, R., Raven Press, New York. p 3–21.Google Scholar
Lim, D. J. (1986) Functional structure of the organ of Corti: A review. Hearing Research. 22: 117–146.CrossRefGoogle ScholarPubMed
Neugebauer, D. C. and Thurm, U. (1985) Interconnections between the stereovilli of the fish inner ear. Cell and Tissue Research. 240: 449–453.CrossRefGoogle Scholar
Osborne, M. P., Comis, S. D., Pickles, J. O. (1988) Further observations of the fine structure of the tip links between stereocilia of the guinea pig cochlea. Hearing Research 35: 99–108.CrossRefGoogle ScholarPubMed
Pickles, J. O., Comis, S. D. and Osborne, M. P. (1984) Cross-links between stereocilia in the guinea pig organ of Corti, and their possible relation to sensory transduction. Hearing Research 15: 103–112.CrossRefGoogle ScholarPubMed
Pickles, J. O., Comis, S. D. and Osborne, M. P. (1987a) The effect of chronic application of kanamycin on stereocilia and their tip links in hair cells of the guinea pig cochlea. Hearing Research. 29: 237–244.CrossRefGoogle ScholarPubMed
Pickles, J. O., Osborne, M. P. and Comis, S. D. (1987b) The vulnerability of tip links between stereocilia to acoustic trauma in the guinea pig. Hearing Research 25: 173–183.CrossRefGoogle ScholarPubMed
Prieto, J. J. and Merchan, J. A. (1986) Tannic acid staining of the cell coat of the organ of Corti. Hearing Research 24: 237–241.CrossRefGoogle ScholarPubMed
Santi, P. A. and Anderson, C. B. (1986) Alcian blue staining of cochlear hair cell stereocilia and other cochlear tissues. Hearing Research 23: 153–160.CrossRefGoogle ScholarPubMed
Santi, P. A. and Anderson, C. B. (1987) A newly identified surface coat on cochlear hair cells. Hearing Research 27: 47–65.CrossRefGoogle ScholarPubMed
Slepecky, N. and Chamberlain, S. C. (1985) The cell coat of inner ear sensory and supporting cells as demonstrated by ruthenium red. Hearing Research 17: 281–288.CrossRefGoogle ScholarPubMed
Takumida, M., Wersäll, J. and Bagger-Sjöbäck, D. (1988) Stereociliar glycocalyx and interconnections in the guinea pig vestibular organs. Acta Otolaryngologica 106: 130–139.CrossRefGoogle Scholar
Takumida, M., Bagger-Sjöbäck, D., Wersäll, J. and Harada, Y. (1989a) The effect of gentamicin on the glycocalyx and ciliary interconnections in vestibular sensory cells: A high resolution scanning electron microscopic investigation. Hearing Research 37: 163–170.CrossRefGoogle Scholar
Takumida, M., Bagger-Sjöbäck, D., Harada, Y., Lim, D. J. and Wersäll, J. (1989b) Sensory hair fusion and glycocalyx changes after gentamicin exposure in the guinea pig. Acta Otolaryngologica 107: 39–47.CrossRefGoogle ScholarPubMed