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Culture of the organism that causes rhinosporidiosis

Published online by Cambridge University Press:  29 June 2007

Karvita B. Ahluwalia
Karvita B. Ahluwalia*
Affiliation:
Cell Biology and Electron Microscope Section, Department of Biophysics, All India Institute of Medical Sciences, New Delhi – 110 029, India.
*
Address for correspondence: Dr Karvita B. Ahluwalia, Electron Microscope Section, Department of Biophysics, All India Institute of Medical Sciences. New Delhi – 110029, India. Fax: 00-91-11-6862663 email: [email protected]
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Abstract

The causative agent of rhinosporidiosis is a microscopic round body in polypoidal masses that is believed to be the sporangium of a fungus Rhinosporidium seeberi. But fungal aetiology can not be proved with certainty. Attempts to culture the fungus on various media have also been unsuccessful. In a recent study pond water samples, from water in which patients had been bathing, were analysed and a unicellular prokaryotic cyanobacterium Microcystis aeruginosa was isolated. The same cyanobacterium was demonstrated in clinical samples from patients with rhinosporidiosis. Consequent to these findings, attempts were made to culture the causative organism of rhinosporidiosis under conditions that support growth of cyanobacteria.

This study describes a simple method for laboratory culture of this organism. Observations based on laser-scanning confocal microscopy, light and electron microscopy confirm that a cyanobacterium, Microcystis sp. is the causative agent of the disease. Rhinosporidiosis is the first human disease to be shown to be caused by a cyanobacterium. The findings have opened the way for development of therapy.

Keywords

RhinosporidiosisCyanobacteriaCulture
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 113 , Issue 6 , June 1999 , pp. 523 - 528
Copyright
Copyright © JLO (1984) Limited 1999

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References

Ahluwalia, K. B. (1992) New interpretations in rhinosporidiosis, enigmatic disease of last nine decades. Journal of Submicroscopic Cytology and Pathology 24: 109–114.Google Scholar
Ahluwalia, K. B., Bahadur, S. (1990) Rhinosporidiosis associated with squamous cell carcinoma in the tongue. Journal of Laryngology and Otology 104: 648–650.Google Scholar
Ahluwalia, K. B., Maheshwari, N., Deka, R. C. (1997) Rhinosporidiosis: a study that resolves etiologic controversies. American Journal of Rhinology 11: 479–483.Google Scholar
Asayama, M., Kabasawa, M., Takahashi, I., Aida, T., Shirai, M. (1996) Highly repetitive sequences and characteristics of genomic DNA in unicellular cyanobacterial strains. FEMS Microbiology Letters 137: 175–181.Google Scholar
Ashworth, J. H. (1923) On Rhinosporidium Wernike 1903, with special reference to its sporulation and affinities. Transactions of the Royal Society, Edinburgh 53: 301–342.Google Scholar
Baron, E. J., Peterson, L. R., Finegold, S. M. (1994) New, controversial, difficult-to-cultivate, or non-cultivable etiological agents of disease. In Bailey and Scott's Diagnostic Microbiology, 9th Edition. Mosby, St Louis, Baltimore, Boston, p. 585.Google Scholar
Bryant, D. A. (1994) The Molecular Biology of Cyanobacteria. Kluwer Academic Publishers, Dordrecht, Boston, p 533.Google Scholar
Carr, N. G., Whitton, B. A. (1982) The Biology of Cyanobacteria. Blackwell Scientific Publications, Oxford, London. pp. 143–189; 387–412.Google Scholar
Castenholz, R. W. (1988) Culturing methods for cyanobacteria. Methods in Enzymology 167: 68–95.Google Scholar
Gaines, J. J., Clay, J. R., Chandler, F. W., Powell, M. E., Sheffield, P. A. (1996) Rhinosporidiosis: three domestic cases. South Medical Journal 89: 65–67.Google Scholar
Ingram, L. O., Fisher, W. D. (1973) Novel mutant impaired in division: evidence for a positive regulating factor. Journal of Bacteriology 113: 999–1005.CrossRefGoogle Scholar
Ingram, L. O., Olson, G. J., Blackwell, M. M. (1975) Isolation of a small-cell mutant in the blue-green bacterium Agmenellum quadruplicatum. Journal of Bacteriology 123: 743–746.Google Scholar
Krishnamoorthy, S., Sreedharan, V. P., Koshy, P., Kumar, S., Anilakumari, C. K. (1989) Culture of Rhinosporidium seeberi: preliminary report. Journal of Laryngology and Otology 103: 178–180.Google Scholar
Kunisawa, R. Cohen-Bazire, G. (1970) Mutants of Anacystis nidulans that affect cell division. Archives of Microbiology 71: 49–59.Google Scholar
Kwon-Chung, K. J., Bennett, J. E. (1992) Medical Mycology. Lea & Febiger, Philadelphia, London, pp 695–706.Google Scholar
Levy, M. G., Meuten, D. J., Breitschwerdt, E. B. (1986) Cultivation of Rhinosporidium seeberi in vitro: interaction with epithelial cells. Science 234: 474–476.Google Scholar
Rippka, R., Druelles, J., Waterbury, J. B., Herdman, M., Stanier, R. Y. (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111: 1–61.Google Scholar
Sakamoto, T., Shirai, M., Asayama, M., Aida, T., Sato, A., Tanaka, K. (1993) Characteristics of DNA and multiple rpoD homologs of Microcystis strains. International Journal of Systematic Bacteriology 43: 844–847.Google Scholar
Satyanarayana, C. (1960) Rhinosporidiosis with a record of 255 cases. Acta Otolaryngologica 51: 348–366.Google Scholar
Shirai, M., Matumaru, K., Ohotake, A., Takamura, Y., Aida, T., Nakano, M. (1989) Development of a solid medium for growth and isolation of axenic Microcystis strain (Cyanobacteria). Applied and Environmental Microbiology 55: 2569–2572.Google Scholar
Sidler, W. A. (1994) Phycobilisome and phycobiliprotein structures. In Molecular Biology of Cyanobacteria, (Bryant, D.A. ed.) Kluwer Academic Publishers, Dordrecht, Boston. pp. 139–216.Google Scholar
Thianprasit, M., Thagerngpol, K. (1989) Rhinosporidiosis. Current Topics in Medical Mycology 3: 64–85.Google Scholar
Vanbreuseghem, R. (1973) Ultrastructure of Rhinosporidium seeberi. International Journal of Dermatology 12: 20–28.Google Scholar
Vukovic, Z., Bobic-Radovanovic, A., Latkovic, Z., Radovanovic, Z. (1995) An epidemiological investigation of the first outbreak of rhinosporidiosis in Europe. Journal of Tropical Medicine and Hygiene 98: 333–337.Google Scholar