Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-24T08:17:37.463Z Has data issue: false hasContentIssue false
  • English
  • Français

Chemiluminescence assay of reactive oxygen species in laryngeal cancer

Published online by Cambridge University Press:  20 May 2010

T Baglam ,
M Sari ,
Z Mine Yazici ,
M Yuksel  and
C Uneri
T Baglam*
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Gaziantep University, Turkey
M Sari
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Marmara University, Istanbul, Turkey
Z Mine Yazici
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Marmara University, Istanbul, Turkey
M Yuksel
Affiliation:
Vocational School of Health Related Sciences, Marmara University, Istanbul, Turkey
C Uneri
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Marmara University, Istanbul, Turkey
*
Address for correspondence: Dr Tekin Baglam, Sahinbey Uygulama ve Arastırma Hastanesi, Kulak Burun Bogaz AD, 27100 Sahinbey, Gaziantep, Turkey. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

This study aimed to evaluate the presence of reactive oxygen species in laryngeal cancer tissue, using a luminol-amplified chemiluminescence method.

Materials and methods:

Fourteen patients with histopathologically diagnosed laryngeal squamous cell carcinoma were enrolled. Patients with recurrent tumours or a history of prior chemotherapy or radiotherapy were excluded. Tissue specimens were harvested both from the tumour itself and from the neighbouring, apparently normal mucosa (immediately after tumour removal). Tissue specimens were washed with ice-cold saline solution and processed immediately, without storage. The level of reactive oxygen species was measured quantitatively by a luminol-amplified chemiluminescence method.

Results:

The mean luminol-amplified chemiluminescence values for tumour and control tissue were 140.52 (standard error of the mean 40.21) and 121.36 (standard error of the mean 35.33) relative light units/mg tissue, respectively. Furthermore, mean tumour and control luminol chemiluminescence values were compared for stage one and two tumours versus stage three and four tumours. Both the tumour and the control luminol chemiluminescence values for the latter tumour group were significantly higher than those for the former tumour group.

Conclusion:

This study measured directly the levels of reactive oxygen species in samples of laryngeal cancer tissue and normal mucosa. Higher levels of reactive oxygen species were found in laryngeal cancer tissue, suggesting a relationship between reactive oxygen species and laryngeal cancer.

Keywords

Larynx CancerReactive Oxygen SpeciesChemiluminescenceLuminol
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 10 , October 2010 , pp. 1091 - 1094
Copyright
Copyright © JLO (1984) Limited 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Koufman, JA, Burke, AJ. The etiology and pathogenesis of laryngeal carcinoma. Otolaryngol Clin North America 1997;30:1–19CrossRefGoogle ScholarPubMed
2Kong, Q, Lillehei, KO. Antioxidant inhibitors for cancer therapy. Medical Hypothesis 1998;51:405–9CrossRefGoogle ScholarPubMed
3Seven, A, Civelek, S, Inci, E, Inci, F, Korkut, N, Burcak, G. Evaluation of oxidative stress parameters in blood of patients with laryngeal carcinoma. Clin Biochem 1999;32:369–73CrossRefGoogle ScholarPubMed
4Taysı, S, Uslu, C, Akçay, F, Sutbeyaz, MY. Malondialdehyde and nitric oxide levels in the plasma of patients with advanced laryngeal cancer. Surgery Today 2003;33:651–4CrossRefGoogle ScholarPubMed
5Kalaycı, A, Ozturk, A, Ozturk, K, Karagozoglu, E, Dolanmaz, D. Superoxide dismutase and gluthatione peroxidase enzyme activity in larynx carcinoma. Acta Otolaryngol 2005;125:312–15CrossRefGoogle Scholar
6Munnia, A, Amasio, ME, Peluso, M. Exocyclic malondialdehyde and aromatic DNA adducts in larynx tissues. Free Radical Biology & Medicine 2004;37:850–8CrossRefGoogle ScholarPubMed
7Piyathilake, CJ, Bell, WC, Oelschlager, DK, Heimburger, DC, Grizzle, WE. The pattern of expression of Mn and Cu-Zn superoxide dismutase varies among squamous cell cancers of the lung, larynx, and oral cavity. Head Neck 2002;24:859–67CrossRefGoogle ScholarPubMed
8Boveris, A, Cadenas, E, Reiter, R, Filipkowski, M, Nakase, Y, Chance, B. Organ chemiluminescence: non-invasive assay for oxidative radical reactions. Proc Natl Acad Sci U S A 1980;77:347–51CrossRefGoogle Scholar
9Feig, DI, Reid, TM, Loeb, LA. Reactive oxygen species in tumorigenesis. Cancer Res 1994;54(suppl 7):1890–4Google ScholarPubMed
10Gutteridge, JM, Halliwell, B. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem Sci 1990;15:129–35CrossRefGoogle ScholarPubMed
11Van Dyke, K, Castranova, V, eds. Cellular Chemiluminescence. London: CRC Press, 1987;1–67Google Scholar
12Panda, K, Chattopadhyay, R, Chattopadhyay, D, Chatterjee, IB. Vitamin C prevents cigarette smoke-induced oxidative damage in vivo. Free Rad Biol Med 2000;29:115–24CrossRefGoogle ScholarPubMed
13Uneri, C, Sarı, M, Baglam, T, Polat, S, Yuksel, M. Effects of vitamin E on cigarette smoke induced oxidative damage in larynx and lung. Laryngoscope 2006;116:97–100CrossRefGoogle ScholarPubMed