Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-28T00:39:12.707Z Has data issue: false hasContentIssue false
  • English
  • Français

Blebs in inner and outer hair cells: a pathophysiological hypothesis

Published online by Cambridge University Press:  10 January 2008

R Ramírez-Camacho ,
J R García-Berrocal ,
A Trinidad ,
J M Verdaguer  and
J Nevado
R Ramírez-Camacho
Affiliation:
Ear Research Group, Department of Otorhinolaryngology, Hospital Universitario Puerta de Hierro, Autónoma University of Madrid, Spain
J R García-Berrocal
Affiliation:
Ear Research Group, Department of Otorhinolaryngology, Hospital Universitario Puerta de Hierro, Autónoma University of Madrid, Spain
A Trinidad*
Affiliation:
Ear Research Group, Department of Otorhinolaryngology, Hospital Universitario Puerta de Hierro, Autónoma University of Madrid, Spain
J M Verdaguer
Affiliation:
Ear Research Group, Department of Otorhinolaryngology, Hospital Universitario Puerta de Hierro, Autónoma University of Madrid, Spain
J Nevado
Affiliation:
Department of Otorhinolaryngology, Hospital Universitario de Getafe, Complutense University of Madrid, Spain
*
Address for correspondence: Dr Almudena Trinidad, Servicio de ORL, Hospital Universitario Puerta de Hierro, San Martín de Porres 4, 28035 Madrid, Spain. Fax: +34 1 3730535 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Introduction:

The ototoxic effects of cisplatin include loss of outer hair cells, degeneration of the stria vascularis and a decrease in the number of spiral ganglion cells. Scanning microscopy has shown balloon-like protrusions (blebs) of the plasma membrane of inner hair cells following cisplatin administration. The present study was undertaken to identify the possible role of inner and outer hair cell blebs in the pathogenesis of cisplatin-induced ototoxicity.

Materials and methods:

Twenty-five guinea pigs were injected with cisplatin and their hearing tested at different time-points, before sacrifice and examination with scanning electron microscopy.

Results and analysis:

Seven animals showed blebs in the inner hair cells at different stages. Hearing thresholds were lower in animals showing blebs.

Discussion:

Cisplatin seems to be able to induce changes in inner hair cells as well as in other structures in the organ of Corti. Blebbing observed in animals following cisplatin administration could play a specific role in the regulation of intracellular pressure.

Keywords

OtotoxicityInner EarCell NecrosisCell ApoptosisCisplatin
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 11 , November 2008 , pp. 1151 - 1155
Copyright
Copyright © JLO (1984) Limited 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Schaefer, SD, Wright, CG, Post, JD, Frenkel, EP. Cis-platinum vestibular toxicity. Cancer 1981;47:857–93.0.CO;2-M>CrossRefGoogle ScholarPubMed
2 Waters, GS, Ahmad, M, Katsarkas, A, Stanimir, G, McKay, J. Ototoxicity due to cis-diaminedichloroplatinum in the treatment of ovarian cancer: influence of dosage and schedule of administration. Ear Hear 1991;12:91–102CrossRefGoogle ScholarPubMed
3 Wright, CG, Schaeffer, SD. Inner ear histopathology in patients treated with cis-platinum. Laryngoscope 1982;92:1408–13CrossRefGoogle ScholarPubMed
4 Hoistad, DL, Ondrey, FG, Mutlu, C, Schachern, PA, Paparella, MM, Adams, GL. Histopathology of human temporal bone after cis-platinum, radiation or both. Otolaryngol Head Neck Surg 1998;118:825–32CrossRefGoogle ScholarPubMed
5 Boulikas, T, Vougiouka, M. Cisplatin and platinum drugs at the molecular level. Oncol Rep 2003;10:1663–82Google ScholarPubMed
6 Alam, SA, Ikeda, K, Oshima, T, Suzuki, M, Kawase, T, Kikuchi, T et al. Cisplatin-induced apoptotic cell death in Mongolian gerbil cochlea. Hear Res 2000;141:28–38CrossRefGoogle ScholarPubMed
7 Devarajan, P, Savoca, M, Castaneda, MP, Parks, MS, Esteban-Cruciani, N, Kalinec, G et al. Cisplatin-induced apoptosis in auditory cells: role of death receptor and mitochondrial pathways. Hear Res 2002;174:45–54CrossRefGoogle ScholarPubMed
8 Zhang, M, Liu, W, Ding, D, Salvi, R. Pifithrin-alpha suppresses p53 and protects cochlear and vestibular hair cells from cisplatin-induced apoptosis. Neuroscience 2003;120:191–205CrossRefGoogle ScholarPubMed
9 Liang, F, Schulte, BA, Qu, C, Hu, W, Shen, Z. Inhibition of the calcium and voltage-dependent big conductance potassium channel ameliorates cisplatin-induced apoptosis in spiral ligament fibrocytes of the cochlea. Neuroscience 2005;135:263–71CrossRefGoogle ScholarPubMed
10 Cheng, PW, Liu, SH, Hsu, CJ, Lin-Shiau, SY. Correlation of increased activities of Na+, K+ ATPase and Ca2+ ATPase with the reversal of cisplatin ototoxicity induced by D-methionine in guinea pigs. Hear Res 2005;205:102–9CrossRefGoogle ScholarPubMed
11 Clerici, WJ, Di Martino, DL, Prasad, MR. Direct effects of reactive oxygen species on cochlear outer hair cell shape in vitro. Hear Res 1995;84:30–40CrossRefGoogle ScholarPubMed
12 Husain, K, Scott, RB, Whitworth, C, Somani, SM, Rybak, LP. Dose response of carboplatin-induced hearing loss in rat. Hear Res 2001;151:71–8CrossRefGoogle Scholar
13 Rybak, LD, Husain, K, Morris, C, Whitworth, C, Somani, S. Effect of protective agents against cisplatin ototoxicity. Am J Otol 2000;21:513–20Google ScholarPubMed
14 Porter, K, Prescott, D, Frye, J. Changes in surface morphology of Chinese hamster during the cell cycle. J Cell Biol 1973;57:815–36CrossRefGoogle ScholarPubMed
15 Trinkaus, JP. Formation of protrusions of the cell surface during tissue cell movement. Prog Clin Biol Res 1980;41:887–906Google ScholarPubMed
16 Zeligs, JD, Wollman, SH. Ultrastructure of blebbing and phagocytosis of blebs by hyperplastic thyroid epithelial cells in vivo. J Cell Biol 1977;72:584–94CrossRefGoogle ScholarPubMed
17 Okada, Y, Maeno, E, Shimizu, T, Dezaki, K, Wang, J, Morishima, S. Receptor-mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD). J Physiol 2001;532:3–16CrossRefGoogle ScholarPubMed
18 Coleman, ML, Sahai, EA, Yeo, M, Bosch, M, Dewar, A, Olson, MF. Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I. Nat Cell Biol 2001;3:339–46CrossRefGoogle ScholarPubMed
19 Krause, WJ, Cutts, JH. Scanning electron microscopic observations on the 9-day opossum (Didelphis virginiana) embryo [in English]. Acta Anat (Basel) 1984;120:93–7CrossRefGoogle ScholarPubMed
20 Olson, EC. Onset of electrical excitability during a period of circus plasma membrane movements in differentiating Xenopus neurons. J Neurosci 1996;16:5117–29CrossRefGoogle ScholarPubMed
21 Moffat, DA. Ototoxicity. In: Kerr, A. Scott-Brown's Otolaryngology. Frome, Somerset: Butterworth, 1987;3:465–99Google Scholar
22 Friedmann, I. Pathology of the cochlea. In: Kerr, A, ed. Scott-Brown's Otolaryngology. Frome, Somerset: Butterworth, 1987;3:88–125Google Scholar
23 Shi, X, Gillespie, PG, Nuttall, AL. Na+ influx triggers bleb formation on inner ear hair cells. Am J Physiol Cell Physiol 2005;288:1332–41CrossRefGoogle Scholar
24 Housley, GD, Raybould, NP, Thorne, PR. Fluorescence imaging of Na+ influx via P2X2 receptors in cochlear hair cells. Hear Res 1998;119:1–13CrossRefGoogle ScholarPubMed
25 Zeddies, DG, Siegel, JH. A biophysical model of an inner hair cell. J Acoust Soc Am 2004;116:426–41CrossRefGoogle ScholarPubMed
26 Ibsch, M, Anken, RH, Vöhringer, P, Rahmann, H. Vesicular bodies in fish maculae are artefacts not contributing to otolith growth. Hear Res 2001;153:80–90CrossRefGoogle Scholar
27 Ramírez-Camacho, R, García-Berrocal, JR, Buján, J, Martín-Marero, A, Trinidad, A. Supporting cells as a target of cisplatin-induced inner ear damage: therapeutic implications. Laryngoscope 2004;114:533–7CrossRefGoogle ScholarPubMed
28 Ramírez-Camacho, R, García-Berrocal, JR, Trinidad, A, Martín-Marero, A, Buján, J. Cochlear cytotoxic activity of cisplatin in experimentation animals. A study using electron microscopy [in Spanish]. Acta Otorrinolaringol Esp 2002;53:407–10Google ScholarPubMed