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Bacterial attachment in vivo to epithelial cells of the nasopharynx during otitis media with effusion

Published online by Cambridge University Press:  29 June 2007

Lars-Eric Stenfors  and
Simo Räisänen
Lars-Eric Stenfors*
Affiliation:
Department of Otolaryngology, Central Hospital of Keski-Pohjanmaa, Kokkola, Finland.
Simo Räisänen
Affiliation:
Clinical Laboratory, Central Hospital of Keski-Pohjanmaa, Kokkola, Finland.
*
Lars-Eric Sternfors, M.D., Department of Otolaryngology, Central Hospital of Keski-Pohjanamaa, SF-37200 Kokkola, Finland.
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Abstract

Epithelial cells were obtained by swabbing the posterior wall of the nasopharynx (NPH) of 15 patients (age one to 6 8/12; years; eight males, seven females) undergoing ENT-surgery under general anaesthesia for otitis media with effusion (OME). Individually matched, ear healthy children served as controls. Bacteria attached to the non-ciliated cells were calculated according to a method described. Furthermore, quantitative and qualitative bacteriological analyses were performed on samples obtained from mucoid middle ear effusion material as well as from the NPH. All patients and controls harboured middle ear pathogens (S. pneumoniae, H. influenzae, B. catarrhalis, or S. aureus) in the NPH. Only 33 per cent of the patients harboured middle ear pathogens in the middle ear effusion and the same pathogen was invariably found in the corresponding NPH Attachment of bacteria to the non-ciliated cells of the NPH diminished significantly with growing age in the ear healthy control group but not in the OME group. OME is closely correlated tothe presence of middle ear pathogens in the NPH and to attachment of bacteria to the epithelial cells in the NPH.

Keywords

BacteriaNasopharynxOtitis media with effusion
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 106 , Issue 2 , February 1992 , pp. 111 - 115
Copyright
Copyright © JLO (1984) Limited 1992

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References

Carlsson, B., Lundberg, C., Ohlson, K. (1982) Granulocyte proteases in middle ear effusions. Annals of Otology, Rhinology and Laryngology, 91: 76–79.Google Scholar
Granfors, K., Jalkanen, S., Von Essen, R., Lahesmaa-Rantala, R., Isomäki, O., Pekkola-Heino, K., Merilahti-Palo, R., Saario, R., Isomäki, H., Toivanen, A. (1989) Yersinia antigens in synovialfluid cells from patients with reactive arthritis. New England Journal of Medicine, 320: 216–221.CrossRefGoogle ScholarPubMed
Howie, V. M., Ploussard, J. H. (1971) Simultaneous nasopharyngeal and middle ear cultures in otitis media. Pediatric Digest, 2: 31–35.Google Scholar
Ingvarsson, L., Lundgren, K., Ursing, J. (1982) The bacterial flora in the nasopharynx in healthy children. Acta Otolaryngologica, Supplement 386: 94–96.CrossRefGoogle Scholar
Juhn, S. (1982) Studies on middle ear effusions. Laryngoscope, 92: 287–291.CrossRefGoogle ScholarPubMed
Kamme, C. (1971) The aetiology of acute otitis media in children and the relationship between bacterial characters and the clinical course in penicillin V therapy. Thesis, Lund, Sweden.Google Scholar
Kamme, C., Nilsson, N.-I. (1984) Secretory otitis media. Microbiology of the middle ear and the nasopharynx. Scandinavian Journal of Infectious Diseases, 16: 291–296.CrossRefGoogle ScholarPubMed
Lim, D. J., DeMaria, T. (1982) Pathogenesis of otitis media. Bacteriology and immunology. Larynoscope, 92: 278–286.CrossRefGoogle ScholarPubMed
Lim, D. J., DeMaria, T. R., Bakaletz, L. O. (1988) Current concepts of pathogenesis of otitis media: A review. Acta Otolaryngologica, Supplement 458: 174–180.Google Scholar
Lundberg, C., Lönnorth, J. (1979) Bacterial adherence to epithelial cells in the nasopharynx in children. Acta Otolaryngologica, 88: 438–442.CrossRefGoogle ScholarPubMed
Lundberg, C., Lönnroth, J., Nord, C. E. (1982a) Adherence in the colonization of Streptococcus pneumoniae in the nasopharynx in children. Infection, 10: 63–66.Google Scholar
Lundberg, C., Lönnorth, J., Nord, C. E. (1982b) Identification of attached bacteria in the nasopharynx of the child. Infection, 10: 58–62.CrossRefGoogle ScholarPubMed
Prellner, K., Christensen, P., Hovelius, B., Rosen, C. (1984) Nasopharyngeal carriage of bacteria in otitis-prone and non-otitis prone children in day-care centres. Acta Otolaryngologica, 98: 343–350.CrossRefGoogle ScholarPubMed
Principi, N., Marchisio, P., Massironi, E., Grasso, R. M., Filiberti, G. (1989) Prophylaxis of recurrent acute otitis media and middle-ear effusion. American Journal of Diseases of Children, 143: 1414–1418.Google Scholar
Schwartz, R., Rodriguez, J., Mann, R., Khan, W. (1979) The nasopharyngeal culture in acute otitis media. A reappraisal of its usefulness. Journal of American Medical Association, 241: 2170–2173.CrossRefGoogle ScholarPubMed
Stenfors, L.-E., Räisänen, S. (1988) Quantification of bacteria in middle ear effusions. Acta Otolaryngologica, 106: 435–440.CrossRefGoogle ScholarPubMed
Stenfors, L.-E., Räisänen, S. (1989a) Colonization of middle ear pathogens in the nasopharyngeal opening of the Eustachian tube during secretory otitis media. Acta Otolaryngologica, 107: 104–110.Google Scholar
Stenfors, L.-E., Räisänen, S. (1989b) The bacterial flora of the nasopharynx, with special reference to middle ear pathogens. A quantitative study in 20 children. Acta Otolaryngologica, 108: 122–125.Google Scholar
Stenfors, L.-E., Räisänen, S. (1990a) Age-dependent changes in bacterial adherence to epithelial cells of nasopharynx in vivo. Acta Otolaryngologica, 110: 292–299.CrossRefGoogle ScholarPubMed
Stenfors, L.-E., Räisänen, S. (1990b) Quantitative analysis of the bacterial findings in otitis media. Journal of Laryngology, and Otology, 104: 749–757.CrossRefGoogle ScholarPubMed
Stenfors, L.-E., Räisänen, S. (1990c) Occurrence of middle ear pathogens in the nasopharynx of young individuals. A quantitative study in four age groups. Acta Otolaryngologica, 109: 142–148.CrossRefGoogle ScholarPubMed
Stenfors, L.-E., Räisänen, S. (1991) Is attachment of bacteria to the epithelial cells of the nasopharynx the key to otitis media? International Journal of Pediatric Otorhinolaryngology, 22: 1–8.Google Scholar
Sundberg, L., Cederberg, Å., Edén, yyT., Emstson, S. (1981) Bacteriology in secretory otitis media. Acta Otolaryngologica, Supplement 384: 18–25.CrossRefGoogle ScholarPubMed
Veltri, R., Sprinkle, P. (1973) Serous otitis media. Immunoglobulin and lysozyme levels in middle ear fluids and serum. Annals of Otology, Rhinology and Laryngology, 82: 297–301.Google Scholar
Virtanen, S., Lahikainen, E. (1979) Lysozyme activity and immunoglobulins in middle ear effusion fluids in acute purulent otitis media and otitis media with effusion. Scandinavian Journal of Infectious Diseases, 11: 63–67.CrossRefGoogle ScholarPubMed
Thomsen, J., Sederberg-Olsen, J., Balle, V., Vejlsgaard, R., Stangerup, S.-E., Bondeson, G. (1989) Antibiotic treatment of children with secretory otitis media. A randomized, double-blind, placebocontrolled study. Archives of Otolaryngology, Head and Neck Surgery, 115: 44–51Google Scholar