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An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea

Published online by Cambridge University Press:  29 June 2007

A. M. Soliman
A. M. Soliman*
Affiliation:
Department of Otorhinolaryngology, University of Tübingen, Germany (FRG). Dr. Soliman is a Channel-fellow, Alexandia Uiversity, Egypt.
*
A. M. Soliman. M.D. Department of Otorhinotaryngology (HNO). silcherstraβe 5, 7400 Tübingen 1, Germany (FRG).
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Summary

A technique for producing frozen sections from the non-decalcified guinea pig cochlea is described. The sections can be utilised or various immunofluorescent studies, including the detection of humoral anti-bodies against different cellular elements in patients with cochleo-vestibular disorders. The technique assures the preservation of the cochlear architecture and the tissue antigenicity, and it avoids damage to the fine cochlear structural details.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 102 , Issue 3 , March 1988 , pp. 215 - 218
Copyright
Copyright © JLO (1984) Limited 1988

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