Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-30T23:19:14.574Z Has data issue: false hasContentIssue false
  • English
  • Français

Overexpression of cyclin E messenger ribonucleic acid in nasopharyngeal carcinoma correlates with poor prognosis

Published online by Cambridge University Press:  11 March 2009

M-T Ko ,
C-Y Su ,
S-C Huang ,
C-H Chen  and
C-F Hwang
M-T Ko
Affiliation:
Department of Otolaryngology, Chang Gung Memorial Hospital – Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
C-Y Su
Affiliation:
Department of Otolaryngology, Chang Gung Memorial Hospital – Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
S-C Huang
Affiliation:
Department of Pathology, Chang Gung Memorial Hospital – Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
C-H Chen
Affiliation:
Department of Otolaryngology, Chang Gung Memorial Hospital – Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
C-F Hwang*
Affiliation:
Department of Otolaryngology, Chang Gung Memorial Hospital – Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
*
Address for correspondence: Dr Chung-Feng Hwang, Department of Otolaryngology, Chang Gung Memorial Hospital – Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung 833, Taiwan. Fax: +886 7 7313855 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Aims:

S-phase kinase-associated protein 2 is required for the degradation of p27 protein, which is a negative regulator of cyclin E/cyclin-dependent kinase 2 complex. The present study examined the expression of cyclin E, S-phase kinase-associated protein 2 and p27 protein in nasopharyngeal carcinoma.

Methods:

Tissue from 35 cases of nasopharyngeal carcinoma and 10 normal nasopharyngeal tissue samples underwent reverse polymerase chain reaction to detect messenger ribonucleic acid. Immunohistochemical analysis was performed on 29 nasopharyngeal tissue samples in order to detect protein expression.

Results:

Messenger ribonucleic acid expression in the nasopharyngeal carcinoma tissue samples analysed indicated a 1.75-fold change in the amount of S-phase kinase-associated protein 2, a 0.34-fold change in the amount of cyclin E and a 0.31-fold change in the amount of p27 protein, compared with positive controls. High levels of cyclin E significantly correlated with late-stage nasopharyngeal carcinoma (p = 0.009) and a poor overall survival (p = 0.010). Immunohistochemical analysis indicated positive expression of S-phase kinase-associated protein 2 in 16/29 nasopharyngeal tissue samples (55 per cent), of cyclin E in 13/29 samples (45 per cent) and of p27 protein in 17/29 (59 per cent) samples.

Conclusions:

Overexpression of cyclin E messenger ribonucleic acid showed an adverse prognostic significance, correlating with an advanced stage of nasopharyngeal carcinoma and a low overall survival rate.

Keywords

Nasopharyngeal NeoplasmsCyclin ESkp2 proteinp27 Kipl protein
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 123 , Issue 9 , September 2009 , pp. 1021 - 1026
Copyright
Copyright © JLO (1984) Limited 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Carrano, AC, Pagano, M. Role of the F-box protein Skp2 in adhesion-dependent cell cycle progression. J Cell Biol 2001;153:1381–90CrossRefGoogle ScholarPubMed
2 Gstaiger, M, Jordan, R, Lim, M, Catzavelos, C, Mestan, J, Slingerland, J et al. Skp2 is oncogenic and overexpressed in human cancers. Proc Natl Acad Sci U S A 2001;98:5043–8CrossRefGoogle ScholarPubMed
3 Tsvetkov, LM, Yeh, KH, Lee, SJ, Sun, H, Zhang, H. p27(Kip1) ubiquitination and degradation is regulated by the SCF(Skp2) complex through phosphorylated Thr187 in p27. Curr Biol 1999;9:661–4CrossRefGoogle ScholarPubMed
4 Carrano, AC, Eytan, E, Hershko, A, Pagano, M. SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat Cell Biol 1999;1:193–9CrossRefGoogle ScholarPubMed
5 Latres, E, Chiarle, R, Schulman, BA, Pavletich, NP, Pellicer, A, Inghirami, G et al. Role of the F-box protein Skp2 in lymphomagenesis. Proc Natl Acad Sci U S A 2001;98:2515–20CrossRefGoogle ScholarPubMed
6 Chiarle, R, Fan, Y, Piva, R, Boggino, H, Skolnik, J, Novero, D et al. S-phase kinase-associated protein 2 expression in non-Hodgkin's lymphoma inversely correlates with p27 expression and defines cells in S phase. Am J Pathol 2002;160:1457–66CrossRefGoogle ScholarPubMed
7 Yokoi, S, Yasui, K, Saito-Ohara, F, Koshikawa, K, Iizasa, T, Fujisawa, T et al. A novel target gene, SKP2, within the 5p13 amplicon that is frequently detected in small cell lung cancers. Am J Pathol 2002;161:207–16CrossRefGoogle ScholarPubMed
8 Datta, MW, Renshaw, AA, Dutta, A, Hoffman, MA, Loughlin, KR. Evaluation of cyclin expression in testicular germ cell tumors: cyclin E correlates with tumor type, advanced clinical stage, and pulmonary metastasis. Mod Pathol 2000;13:667–72CrossRefGoogle ScholarPubMed
9 Muller-Tidow, C, Metzger, R, Kugler, K, Diederichs, S, Idos, G, Thomas, M et al. Cyclin E is the only cyclin-dependent kinase 2-associated cyclin that predicts metastasis and survival in early stage non-small cell lung cancer. Cancer Res 2001;61:647–53Google ScholarPubMed
10 Vokes, EE, Liebowitz, DN, Weichselbaum, RR. Nasopharyngeal carcinoma. Lancet 1997;350:1087–91CrossRefGoogle ScholarPubMed
11 Fandi, A, Altun, M, Azli, N, Armand, JP, Cvitkovic, E. Nasopharyngeal cancer: epidemiology, staging, and treatment. Semin Oncol 1994;21:382–97Google ScholarPubMed
12 Moore, C, Shen, XD, Gao, F, Busuttil, RW, Coito, AJ. Fibronectin-alpha4beta1 integrin interactions regulate metalloproteinase-9 expression in steatotic liver ischemia and reperfusion injury. Am J Pathol 2007;170:567–77CrossRefGoogle ScholarPubMed
13 Mustafa, T, Klonisch, T, Hombach-Klonisch, S, Kehlen, A, Schmutzler, C, Koehrle, J et al. Expression of CD97 and CD55 in human medullary thyroid carcinomas. Int J Oncol 2004;24:285–94Google ScholarPubMed
14 Sobin, LH, Fleming, ID. TNM Classification of Malignant Tumors, fifth edition (1997). Union Internationale Contre le Cancer and the American Joint Committee on Cancer. Cancer 1997;80:1803–43.0.CO;2-9>CrossRefGoogle ScholarPubMed
15 Hershko, D, Bornstein, G, Ben-Izhak, O, Carrano, A, Pagano, M, Krausz, MM et al. Inverse relation between levels of p27(Kip1) and of its ubiquitin ligase subunit Skp2 in colorectal carcinomas. Cancer 2001;91:1745–513.0.CO;2-H>CrossRefGoogle ScholarPubMed
16 Kudo, Y, Kitajima, S, Sato, S, Miyauchi, M, Ogawa, I, Takata, T. High expression of S-phase kinase-interacting protein 2, human F-box protein, correlates with poor prognosis in oral squamous cell carcinomas. Cancer Res 2001;61:7044–7Google ScholarPubMed
17 Kim, JY, Lim, SJ, Kim, HJ, Shin, E, Park, K, Lee, CM. Clinical significance of p27 and Skp2 protein expression in uterine cervical neoplasm. Int J Gynecol Pathol 2007;26:242–7CrossRefGoogle ScholarPubMed
18 Sui, L, Dong, Y, Watanabe, Y, Yamaguchi, F, Sugimoto, K, Tokuda, M. Clinical significance of Skp2 expression, alone and combined with Jab1 and p27 in epithelial ovarian tumors. Oncol Rep 2006;15:765–71Google ScholarPubMed
19 Fukuse, T, Hirata, T, Naiki, H, Hitomi, S, Wada, H. Prognostic significance of cyclin E overexpression in resected non-small cell lung cancer. Cancer Res 2000;60:242–4Google ScholarPubMed
20 Mishina, T, Dosaka-Akita, H, Hommura, F, Nishi, M, Kojima, T, Ogura, S et al. Cyclin E expression, a potential prognostic marker for non-small cell lung cancers. Clin Cancer Res 2000;6:11–16Google ScholarPubMed
21 Brzezinski, J, Migodzinski, A, Gosek, A, Tazbir, J, Dedecjus, M. Cyclin E expression in papillary thyroid carcinoma: relation to staging. Int J Cancer 2004;109:102–5CrossRefGoogle ScholarPubMed
22 Bani-Hani, KE, Almasri, NM, Khader, YS, Sheyab, FM, Karam, HN. Combined evaluation of expressions of cyclin E and p53 proteins as prognostic factors for patients with gastric cancer. Clin Cancer Res 2005;11:1447–53CrossRefGoogle ScholarPubMed
23 Peters, MG, Vidal Mdel, C, Gimenez, L, Mauro, L, Armanasco, E, Cresta, C et al. Prognostic value of cell cycle regulator molecules in surgically resected stage I and II breast cancer. Oncol Rep 2004;12:1143–50Google Scholar
24 Baba, Y, Tsukuda, M, Mochimatsu, I, Furukawa, S, Kagata, H, Satake, K et al. Reduced expression of p16 and p27 proteins in nasopharyngeal carcinoma. Cancer Detect Prev 2001;25:414–19Google ScholarPubMed
25 Hwang, CF, Su, CY, Huang, SC, Huang, CC, Fang, FM, Lui, CC et al. Low expression levels of p27 correlate with loco-regional recurrence in nasopharyngeal carcinoma. Cancer Lett 2003;189:231–6CrossRefGoogle ScholarPubMed