Hostname: page-component-745bb68f8f-kw2vx Total loading time: 0 Render date: 2025-01-09T08:12:30.987Z Has data issue: false hasContentIssue false
  • English
  • Français

Localization of salivary amylase and epithelial membrane antigen in salivary gland tumours by means of imunoperoxidase and immunogold-silver techniques

Published online by Cambridge University Press:  29 June 2007

M Egan ,
J Crocker  and
P Nar
M Egan*
Affiliation:
Department of Histopathology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
J Crocker
Affiliation:
Department of Histopathology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
P Nar
Affiliation:
Department of Histopathology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
*
Dr. M. Egan, M.B., Ch.B., Dept. of Histopathology, The Royal Hospital, Wolverhampton WV2 1BT.
Get access Rights & Permissions [Opens in a new window]

Summary

A series of 87 salivary glands was examined using an immunogold-silver technique for salivary gland amylase (SGA) and epithelial membrane antigen (EMA) and an indirect immunoperoxidase technique for both antigens. The specimens comprised 21 pleomorphic adednomas, 3 monomorphic adenomas, 25 adenolymphomas, 5 mucoepidermoid carcinomas, 3 acinic cell tumours, 8 adenoid cystic carcinomas, 2 adenocarcinomas and 8 undifferentiated carcinomas. In addition 5 normal salivary glands and 6 salivary glands showing benign lymphoepithelial lesions were included.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 102 , Issue 3 , March 1988 , pp. 242 - 247
Copyright
Copyright © JLO (1984) Limited 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Caselitz, J., Osborn, M., Seifert, G. and Weber, K. (1981) Different intermediate sized filaments proteins in human salivary gland and their tumours. Virchows Archives, 393: 273–286.CrossRefGoogle Scholar
Caselitz, J., Seifert, G., Grenner, G. and Schmidtberger, R. (1983) Amylase as an additional marker of salivary gland neoplasms. Path Res Pract, 176: 276–283.CrossRefGoogle ScholarPubMed
Crocker, J. and Jenkins, R. (1984) Quantitative study of histiocytic reticulum cells in diffuse and follicular non-Hodgkin lymphoma. Journal of Clinical Pathology. 13: 1222–1226.Google Scholar
Crocker, J., Jenkins, R., Campbell, J., Fuggle, W. and Shah, V. (1985) Immunohistochemical demonstration of S-100 protein in salivary gland neoplasms. Journal of Pathology, 146: 115–121.Google Scholar
Dardick, I., van Nostrand, A. and Phillips, M. J. (1982) Histogenesis of salivary gland pleomorphic adenoma: A review evaluating the derivation, nature and comparative role of the myoepithelial cell. Human Pathology, 13: 62–75.Google Scholar
Erlandson, R. A. (1972) Ultrastructure of acinic cell carcinoma of parotid gland. Archives of Pathology, 93: 130–140.Google Scholar
Erlandson, R. A., Cardon-Cardo, C. and Higgins, P. (1984) Histogenesis of benign pleomorphic adenoma (mixed tumour) of major salivary glands: An immunohistochemical and ultrastruc-tural study. American Journal of Surgical Pathology, 8: 803–820.CrossRefGoogle Scholar
Ghadially, F. N. (1980) Diagnostic electron microscopy of tumours. Butterworth, London, pp. 215–219.Google Scholar
Gusterson, B., Lucas, B. and Ormerod, M. (1982) Distribution of epithelial membrane antigen in benign and malignant lesions of salivary glands. Virchows Archives, 397: 227–233.CrossRefGoogle ScholarPubMed
Heyderman, E., Steel, K. and Ormerod, M. (1979) A new antigen on epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue. Journal of Clinical Pathology, 32: 35–39.CrossRefGoogle ScholarPubMed
Hirtzler, R., Oberman, B. and Kulis, M. (1969) Ljubisicic Azinuszelladenocarcinome der speictredrusen. Archiv für Ohren-Naen-Kehlkopfheilkunde, 195: 68–80.Google Scholar
Holgate, C. S., Jackson, P., Lauder, I., Cowen, P. N. and Bird, C. C. (1983) Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkins lymphoma using an immu-nogold-silver staining technique. Journal of Clinical Pathology, 36: 742–6.Google Scholar
Hubner, G., Klein, H. J., Kleinhasser, O. and Schiefer, H. G. (1971) Role of myoepithelial cells in the development of salivary gland tumours. Cancer, 27: 1255–1261.3.0.CO;2-1>CrossRefGoogle Scholar
Johns, M., Mills, S. and Thompson, K. K. (1983) Colony forming assay of human salivary gland tumours. Applications for chemisensitivity and histogenic studies. Archives of Otolarynology, 109: 709–714.CrossRefGoogle Scholar
Kahn, L. (1979) Benign lymphoepithelial lesion (Mikulicz Disease) of the salivary gland: An ultrastructural study. Human Pathology, 10: 99–104.Google Scholar
Kraus, F. and Mestecky, J. (1971) Immunohistochemical localisation of amylase, lysozyme and immunoglobulins in human parotid glands. Archives of Biology, 781–789.Google Scholar
Morgan, W. and Castleman, B. (1953) A clinicopathologic study of ‘Mukulicz Disease’. American Journal of Pathology, 29: 471–503.Google Scholar
Palmer, R. M. and Lucas, R. B. (1985) Immunocytochemical identification of cell types of pleomorphic adenoma, with particular reference to myoepithelial cells. Journal of Oral Pathology, 146: 213–220.Google Scholar
Said, I., Teratan, K., Inous, K., Saito, A., Funatsu, K., Moro, I., (1984) Immunohistochemical characterization of functional markers in human minor salivary gland tumours. Journal of Oral Pathology, 313: 525–534.Google Scholar
Sangster, G., Crocker, J., Nar, P. and Leyland, M. J. (1986) Benign and malignant (B cell) focal lymphoid aggregates in bone marrow trephines shown by an immunogold silver technique. Journal of Clinical Pathology, 39: 453–457.Google Scholar
Seifert, G. (1985) The importance of tumour markers in oral pathology. Path Res Pract, 179: 625–628.Google Scholar
Shirasuna, K., Sato, M. and Miyazaki, T. (1980) A myoepithelial cell line established from human pleomorphic adenoma arising in minor salivary glands. Cancer, 45: 297–3.0.CO;2-R>CrossRefGoogle Scholar
Sloane, J. P. and Ormerod, M. G. (1981) Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology. Cancer, 47: 1786–1795.3.0.CO;2-8>CrossRefGoogle ScholarPubMed
Sloane, J. P., Ormerod, M. G., Gusteson, B. A. and Foster, C. S. (1982) An immunocytochemical study of epithelial membrane antigen in normal and disordered squamous epithelium. Diagnostic Histopathology, 5: 11–17.Google ScholarPubMed
Welsh, R. and Meyer, A. (1968) Mixed tumours of human salivary gland: Histogenesis. Archives of Pathology, 85: 433–447.Google Scholar
Zimmer, K. P., Caselitz, J., Seifert, G. and Grenner, G. (1984) Immunoelectron microscopy of amylase in the human parotid gland. Virchows Archives, 404: 187–196.Google Scholar