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Increase in epithelial mast cell numbers in the nasal mucosa of patients with perennial allergic rhinitis

Published online by Cambridge University Press:  29 June 2007

A. Slater ,
L. A. Smallman  and
A. B. Drake-Lee
A. Slater*
Affiliation:
Department of Pathology, The Medical School, University of Birmingham, Birmingham, UK.
L. A. Smallman
Affiliation:
Department of Pathology, The Medical School, University of Birmingham, Birmingham, UK.
A. B. Drake-Lee
Affiliation:
Department of Otolaryngology, The Queen Elizabeth Hospital, Birmingham, UK.
*
Address for correspondence: Andrew Slater, 96 Warwards Lane, Selly Park, Birmingham B29 7RD.
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Abstract

The aim of the study was to compare the numbers and distribution of mast cells in the nasal mucosa of perennial allergic rhinitis (PAR) patients and controls, as demonstrated by different staining methods for light microscopy.

Biopsies of inferior turbinate mucosa were taken from 10 patients with PAR and 10 patients undergoing septoplasty or septorhinoplasty (control group). Sections for light microscopy were stained with azure A, chloroacetate esterase and an ABC immunohistochemical technique using antibody to tryptase.

Three times more mast cells were found in the epithelium of PAR patients compared to controls using the immunohistochemical technique (p = 0.0074). This method demonstrated considerably more mast cells than the other stains.

The increase in epithelial mast cells is consistent with the migration of mast cells seen in seasonal allergic rhinitis, and this may be important in the phenomenon of nasal priming seen after repeated antigen exposure.

Keywords

ImmunohistochemistryMast cells, Rhinitis, allergic, perennial
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 110 , Issue 10 , October 1996 , pp. 929 - 933
Copyright
Copyright © JLO (1984) Limited 1996

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Footnotes

This work has been presented at a meeting of the Midland Institute of Otolaryngology at City Hospital, Birmingham in January, 1994 and as a poster to the Winter Meeting of the Pathological Society of Great Britain and Ireland at the John Radcliffe Hospital, Oxford in January 1995.

References

Barrett, K. E., Metcalfe, D. D. (1987) Heterogeneity of mast cells in the tissues of the respiratory tract and other organ systems. American Review of Respiratory Disease 135: 1190–1195.Google ScholarPubMed
Bascom, R., Pipkorn, U., Lichtenstein, L. M., Naclerio, R. M. (1988) The influx of inflammatory cells into nasal washings during the late response to antigen challenge. Effect of steroid pretreatment. American Review of Respiratory Disease 138: 406–412.CrossRefGoogle ScholarPubMed
Bentley, A. M., Jacobson, M. R., Cumberworth, V., Barkans, J. R., Moqbel, R., Schwartz, L. B., Irani, A. A., Kay, A. B., Durham, S. R. (1992) Immunohistology of the nasal mucosa in seasonal allergic rhinitis: increases in activated eosinophils and epithelial mast cells. Journal of Allergy and Clinical Immunology 89: 877–883.CrossRefGoogle ScholarPubMed
Bland, J. M., Altman, D. G. (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet I: 307–310.CrossRefGoogle Scholar
Cook, H. C. (1982) Carbohydrates. In Theory and Practice of Histological Techniques. 2nd Edition. (Bancroft, J. D., Stevens, A., eds.), Churchill Livingstone, London, pp 180–216.Google Scholar
Craig, S. S., DeBlois, G., Schwarz, L. B. (1986) Mast cells in human keloid, small intestine, and lung by an immunoperoxidase technique using a murine monoclonal antibody against tryptase. American Journal of Pathology 124: 427–435.Google Scholar
Drake-Lee, A. B., Moriarty, B., Smallman, L. A. (1991) Mast cell numbers in the mucosa of the inferior turbinate in patients with perennial allergic rhinitis: a light microscopy study. Journal of Laryngology and Otology 105: 736–738.Google Scholar
Drake-Lee, A. B., Price, J. (1992) A review of the morphology of human nasal mast cells as studied by light and electron microscopy. Rhinology 30: 229–239.Google ScholarPubMed
Enerback, L., Pipkorn, U., Granerus, G. (1986) Intraepithelial migration of nasal mucosal mastcells in hay fever. International Archives of Allergy and Applied Immunology 80: 44–51.CrossRefGoogle Scholar
Fokkens, W. J., Godthelp, T., Holm, A. F., Blom, H., Mulder, P. G. H., Vroom, T. M., Rijntjes, E. (1992) Dynamics of mast cells in the nasal mucosa of patients with allergic rhinitis and non-allergic controls: a biopsy study. Clinical and Experimental Allergy 22: 701–710.CrossRefGoogle ScholarPubMed
Gomez, E., Clague, J. E., Gatland, D., Davies, R. J. (1988) Effect of topical corticosteroids on seasonally induced increases in nasal mast cells. British Medical Journal 296: 1572–1573.CrossRefGoogle ScholarPubMed
Hopwood, D. (1982) Fixation and fixatives. In Theory and Practice of Histological Techniques. 2nd Edition. (Bancroft, J. D., Stevens, A., eds.) Churchill Livingstone, London, pp 20–39.Google Scholar
Igarashi, Y., Kaliner, M. A., Hausfeld, J. N., Irani, A. A., Schwartz, L. B., White, M. V. (1993) Quantification of resident inflammatory cells in the human nasal mucosa. Journal of Allergy and Clinical Immunology 91: 1082–1093.CrossRefGoogle ScholarPubMed
Irani, A. A., Bradford, J. R., Kepley, C. L., Schechter, N. M., Schwartz, L. B. (1989) Detection of MCT and MCTC types of human mast cells by immunohistochemistry using new monoclonal anti-tryptase and anti-chymase antibodies. Journal of Histochemistry and Cytochemistry 37:1509–1515.CrossRefGoogle ScholarPubMed
Irani, A. A., Schechter, N. M., Craig, S. S., Deblois, G., Schwartz, L. B. (1986) Two types of human mast cells that have distinct neutral protease compositions. Proceedings of the National Academy of Science USA 83: 4464–4468.CrossRefGoogle ScholarPubMed
Juliusson, S., Holmberg, K., Karlsson, G., Enerback, L., Pipkorn, U. (1993) Mast cells and mediators in the nasal mucosa after allergen challenge. Effect of four weeks'treatment with topical glucocorticoid. Clinical and Experimental Allergy 23: 591–599.CrossRefGoogle ScholarPubMed
Kaliner, M. (1992) Rhinitis and asthma. Journal of the American Medical Association 268: 2807–2829.Google Scholar
Kawabori, S., Okuda, M., Unno, T. (1983) Mast cells in allergic nasal epithelium and lamina propria before and after provocation. Clinical Allergy 13: 181–189.Google Scholar
Kitamura, Y. (1989) Heterogeneity of mast cells and phenotypic change between subpopulations. Annual Review of Immunology 7: 59–76.CrossRefGoogle ScholarPubMed
Li, C. Y., Lam, K. W., Yam, L. T. (1973) Esterases in human leukocytes. Journal of Histochemistry and Cytochemistry 21: 1–12.CrossRefGoogle ScholarPubMed
Morgan, S. J., William, J. H. (1991) Mast cell numbers and staining characteristics in the normal and allergic human conjunctiva. Journal of Allergy and Clinical Immunology 87: 111–116.CrossRefGoogle ScholarPubMed
Okuda, M., Ohtsuka, H., Kawabori, S. (1983) Basophil leukocytes and mast cells in the nose. European Journal of Respiratory Diseases 64 (suppl 128): 7–14.Google Scholar
Otsuka, H., Ohkuba, K., Seki, H., Ohnishi, M., Fujikura, T. (1993) Mast cell quantitation in nasal polyps, sinus mucosa and nasal turbinate mucosa. Journal of Laryngology and Otology 107: 418–22.CrossRefGoogle ScholarPubMed
Otsuka, H., Denburg, J., Dolovich, J., Hitch, D., Lapp, P., Rajan, R. S., Beinenstock, J., Befus, D. (1985) Heterogeneity of metachromatic cells in the human nose: significance of mucosal mast cells. Journal of Allergy and Clinical Immunology 76: 695–702.Google Scholar
Pearce, F. L., Befus, A. D., Gauldie, J., Bienenstock, J. (1982) Mucosal mast cells: ii. Effects of anti-allergic compounds on histamine secretion by isolated intestinal mast cells. Journal of Immunology 128: 2481–2486.CrossRefGoogle ScholarPubMed
Pesci, A., Foresi, A., Bertorelli, G., Chetta, A., Oliveri, D. (1993) Histochemical characteristics and degranulation of mast cells in epithelium and lamina propria of bronchial biopsies from asthmatic and normal patients. American Review of Respiratory Disease 147: 684–689.Google Scholar
Peters, S. P., Schleimer, R. P., Naclerio, R. M., MacGlashan, D. W. Jr., Togias, A. G., Proud, D., Freeman, H. S., Fox, C., Adkinson, N. F. Jr., Lichtenstein, L. M. (1987) The pathophysiology of human mast cells. American Review of Respiratory Disease 135: 1196–1200.Google ScholarPubMed
Pipkorn, U., Enerback, L. (1987a) Nasal mucosal mast cells and histamine in hay fever: Effect of topical glucocorticoid treatment. International Archives of Allergy and Applied Immunology 84: 123–128.CrossRefGoogle ScholarPubMed
Pipkorn, U., Proud, D., Lichtenstein, L. M., Schleimer, R. P., Peters, S. P., Adkinson, N. F. Jr., Kagey-Sobotka, A., Norman, P. S., Naclerio, R. M. (1987b) Effect of short-term systemic glucocorticoid treatment on human nasal mediator release after antigen challenge. Journal of Clinical Investigation 80: 957–961.CrossRefGoogle ScholarPubMed
Pipkorn, U., Karlsson, G., Enerback, L. (1988) Phenotypic expression of proteoglycan in mast cells of the human nasal mucosa. Histochemical Journal 20: 519–525.CrossRefGoogle ScholarPubMed
Schwartz, L. B., Bradford, T. R., Irani, A. A., Deblois, G., Craig, S. S. (1987) The major enzymes of human mast cell secretory granules. American Review of Respiratory Disease 135: 1186–1189.Google Scholar
Strobel, S., Miller, H. R. P., Ferguson, A. (1981) Human intestinal mucosal mast cells: evaluation of fixation and staining techniques. Journal of Clinical Pathology 34: 851–858.Google Scholar
Viegas, M., Gomez, E., Brooks, J., Gatland, D., Davies, R. J. (1987) Effect of the pollen season on nasal mast cell numbers. British Medical Journal 294: 414.CrossRefGoogle Scholar
Walls, A. F., Bennett, A. R., McBride, H. M., Glennie, M. J., Holgate, S. T., Church, M. K. (1990a) Production and characterisation of monoclonal antibodies specific for human mast cell tryptase. Clinical and Experimental Allergy 20: 581–589.CrossRefGoogle ScholarPubMed
Walls, A. F., Jones, D. B., Williams, J. H., Church, M. K., Holgate, S. T. (1990b) Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase. Journal of Pathology 162: 119–126.CrossRefGoogle ScholarPubMed