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Immunolocalisation of heme oxygenase isoforms in human nasal polyps

Published online by Cambridge University Press:  07 January 2008

S Lo ,
S Di Palma ,
E George  and
A W McCombe
S Lo*
Affiliation:
Department of Otorhinolaryngology, St George's Hospital Medical School, London, UK
S Di Palma
Affiliation:
Department of Histopathology, Royal Surrey County Hospital, Guildford, UK
E George
Affiliation:
Department of Otorhinolaryngology, Frimley Park Hospital, Frimley, UK
A W McCombe
Affiliation:
Department of Otorhinolaryngology, Frimley Park Hospital, Frimley, UK
*
Address for correspondence: Mr Stephen Lo, 4 Osborne Road Farnborough GU14 6PT, UK. Fax: (+44) (0)208 725 3306 E-mail: [email protected]
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Abstract

Background:

Carbon monoxide is an endogenous vasodilator gas produced by the enzyme heme oxygenase (HO). HO is expressed in human nasal mucosa, but its pathophysiological role in nasal inflammatory diseases is not fully understood. The aim of this study was to detect and compare the expression of HO-1 and -2 isoforms in nasal polyps with normal nasal mucosa.

Methods:

Immunohistochemical analysis using antibodies specific for HO-1 and -2 was conducted on nasal polyps from nine patients with allergic nasal polyposis, and on normal nasal mucosa from six controls.

Results:

Intense HO-1 immunoreactivity was observed in nasal polyp epithelium but was absent in normal nasal mucosa. HO-2 staining was observed in respiratory epithelium, vascular endothelium and seromucous glands, with no difference observed between nasal polyps and normal nasal mucosa.

Conclusions:

HO-1 expression is up-regulated in nasal polyp epithelium, supporting the theory that respiratory epithelium plays a role in the pathogenesis of nasal polyposis.

Keywords

Heme OxygenaseCarbon MonoxideNasal PolypsImmunohistochemistry
Type
Clinical Records
Information
The Journal of Laryngology & Otology , Volume 123 , Issue 1 , January 2009 , pp. 131 - 135
Copyright
Copyright © JLO (1984) Limited 2008

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References

1 Klossek, JM, Neukirch, F, Pribil, C, Jankowski, R, Serrano, E, Chanal, I et al. Prevalence of nasal polyposis in France: a cross-sectional, case-control study. Allergy 2005;60:233–7CrossRefGoogle Scholar
2 Johansson, L, Akerlund, A, Holmberg, K, Melen, I, Bende, M. Prevalence of nasal polyps in adults: the Skovde population-based study. Ann Otol Rhinol Laryngol 2003;112:625–9CrossRefGoogle ScholarPubMed
3 Peters-Golden, M, Henderson, WR Jr. The role of leukotrienes in allergic rhinitis. Ann Allergy Asthma Immunol 2005;94:609–18, 618–20, 669CrossRefGoogle ScholarPubMed
4 Gelfand, EW. Inflammatory mediators in allergic rhinitis. J Allergy Clin Immunol 2004;114(suppl 5):S135–8CrossRefGoogle ScholarPubMed
5 Bradley, DT, Kountakis, SE. Role of interleukins and transforming growth factor-beta in chronic rhinosinusitis and nasal polyposis. Laryngoscope 2005;115:684–6CrossRefGoogle ScholarPubMed
6 Bhandari, A, Takeuchi, K, Suzuki, S, Harada, T, Hayashi, S, Imanaka-Yoshida, K et al. Increased expression of matrix metalloproteinase-2 in nasal polyps. Acta Otolaryngol 2004;124:1165–70CrossRefGoogle ScholarPubMed
7 Fang, SY, Shen, CL, Ohyama, M. Presence of neuropeptides in human nasal polyps. Acta Otolaryngol 1994;114:324–8CrossRefGoogle ScholarPubMed
8 Watkins, DN, Lewis, RH, Basclain, KA, Fisher, PH, Peroni, DJ, Garlepp, MJ et al. Expression and localization of the inducible isoform of nitric oxide synthase in nasal polyp epithelium. Clin Exp Allergy 1998;28:211–19CrossRefGoogle ScholarPubMed
9 Gilain, L, Bedu, M, Jouaville, L, Guichard, C, Advenier, D, Mom, T et al. Analysis of nasal and exhaled nitric oxide concentration in nasal polyposis. Ann Otolaryngol Chir Cervicofac 2002;119:234–42Google ScholarPubMed
10 Kang, BH, Huang, NC, Wang, HW. Possible involvement of nitric oxide and peroxynitrite in nasal polyposis. Am J Rhinol 2004;18:191–6CrossRefGoogle ScholarPubMed
11 Rodgers, PA, Vreman, HJ, Dennery, PA, Stevenson, DK. Sources of carbon monoxide (CO) in biological systems and applications of CO detection technologies. Semin Perinatol 1994;18:2–10Google Scholar
12 Elbirt, KK, Bonkovsky, HL. Heme oxygenase: recent advances in understanding its regulation and role. Proc Assoc Am Physicians 1999;111:438–47CrossRefGoogle ScholarPubMed
13 Maines, MD. The heme oxygenase system and its functions in the brain. Cell Mol Biol 2000;46:573–85Google ScholarPubMed
14 McCoubrey, WK Jr, Huang, TJ, Maines, MD. Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3. Eur J Biochem 1997;247:725–32CrossRefGoogle ScholarPubMed
15 Horvath, I, Loukides, S, Wodehouse, T, Kharitonov, SA, Cole, PJ, Barnes, PJ. Increased levels of exhaled carbon monoxide in bronchiectasis: a new marker of oxidative stress. Thorax 1998;53:867–70CrossRefGoogle Scholar
16 Horvath, I, Borka, P, Apor, P, Kollai, M. Exhaled carbon monoxide concentration increases after exercise in children with cystic fibrosis. Acta Physiol Hung 1999;86:237–44Google ScholarPubMed
17 Paredi, P, Leckie, MJ, Horvath, I, Allegra, L, Kharitonov, SA, Barnes, PJ. Exhaled carbon monoxide is elevated during allergen challenge in asthmatic patients. Eur Respir J 1998;28(suppl):127–8Google Scholar
18 Andersson, JA, Uddman, R, Cardell, LO. Carbon monoxide is endogenously produced in the human nose and paranasal sinuses. J Allergy Clin Immunol 2000;105:269–73CrossRefGoogle ScholarPubMed
19 Lo, S, Di Palma, S, Pitkin, L, McCombe, AW. Localisation of heme oxygenase isoforms in allergic human nasal mucosa. Eur Arch Otorhinolaryngol 2005;262:595–8CrossRefGoogle Scholar
20 Andersson, JA, Uddman, R, Cardell, LO. Increased carbon monoxide levels in the nasal airways of subjects with a history of seasonal allergic rhinitis and in patients with upper respiratory tract infection. Clin Exp Allergy 2002;32:224–7CrossRefGoogle ScholarPubMed
21 Brennan, PA, Umar, T, Buckley, J, Zaki, GA, Langdon, JD, Spedding, A et al. Expression of nitric oxide synthase in pleomorphic adenomas of the parotid. Br J Oral Maxillofac Surg 2000;38:338–42CrossRefGoogle ScholarPubMed
22 Donnelly, LE, Barnes, PJ. Expression of heme oxygenase in human airway epithelial cells. Am J Respir Cell Mol Biol 2001;24:295–303CrossRefGoogle ScholarPubMed
23 Lim, S, Groneberg, D, Fischer, A, Oates, T, Caramori, G, Mattos, W et al. Expression of heme oxygenase isoenzymes 1 and 2 in normal and asthmatic airways: effect of inhaled corticosteroids. Am J Respir Crit Care Med 2000;162:1912–18CrossRefGoogle ScholarPubMed
24 Lakari, E, Pylkas, P, Pietarinen-Runtti, P, Paakko, P, Soini, Y, Kinnula, VL. Expression and regulation of hemoxygenase 1 in healthy human lung and interstitial lung disorders. Hum Pathol 2001;32:1257–63CrossRefGoogle Scholar
25 Mumby, S, Upton, RL, Chen, Y, Stanford, SJ, Quinlan, GJ, Nicholson, AG et al. Lung heme oxygenase-1 is elevated in acute respiratory distress syndrome. Crit Care Med 2004;32:1130–5CrossRefGoogle ScholarPubMed
26 Lundberg, JO, Palm, J, Alving, K. Nitric oxide but not carbon monoxide is continuously released in the human nasal airways. Eur Respir J 2002;20:100–3CrossRefGoogle Scholar
27 Monma, M, Yamaya, M, Sekizawa, K, Ikeda, K, Suzuki, N, Kikuchi, T et al. Increased carbon monoxide in exhaled air of patients with seasonal allergic rhinitis. Clin Exp Allergy 1999;29:1537–41CrossRefGoogle ScholarPubMed
28 Yamaya, M, Sekizawa, K, Ishizuka, S, Monma, M, Mizuta, K, Sasaki, H. Increased carbon monoxide in exhaled air of subjects with upper respiratory tract infections. Am J Respir Crit Care Med 1998;158:311–14CrossRefGoogle Scholar
29 Seaton, A, MacNee, W, Donaldson, K, Godden, D. Particulate air pollution and acute health effects. Lancet 1995;345:176–8CrossRefGoogle ScholarPubMed
30 Li, XY, Gilmour, PS, Donaldson, K, MacNee, W. Free radical activity and pro-inflammatory effects of particulate air pollution (PM10) in vivo and in vitro. Thorax 1996;51:1216–22CrossRefGoogle ScholarPubMed
31 Djukanovic, R. Nasal polyps – a model of chronic respiratory mucosal inflammation. Clin Exp Allergy 1995;25:582–5CrossRefGoogle Scholar
32 Durante, W, Kroll, MH, Christodoulides, N, Peyton, KJ, Schafer, AI. Nitric oxide induces heme oxygenase-1 gene expression and carbon monoxide production in vascular smooth muscle cells. Circ Res 1997;80:557–64CrossRefGoogle ScholarPubMed
33 Harju, T, Soini, Y, Paakko, R, Kinnula, VL. Up-regulation of heme oxygenase-I in alveolar macrophages of newly diagnosed asthmatics. Respir Med 2002;96:418–23CrossRefGoogle ScholarPubMed
34 Slavin, RG. Relationship of nasal disease and sinusitis to bronchial asthma. Ann Allergy 1982;49:76–9Google Scholar