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Head and neck squamous cell carcinoma is not associated with interleukin-18 promoter gene polymorphisms: a case–control study

Published online by Cambridge University Press:  22 October 2008

V Asefi ,
Z Mojtahedi ,
B Khademi ,
S Naeimi  and
A Ghaderi
V Asefi
Affiliation:
Division of ENT, Namazee Hospital, Iran
Z Mojtahedi
Affiliation:
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Iran
B Khademi
Affiliation:
Division of ENT, Namazee Hospital, Iran
S Naeimi
Affiliation:
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Iran
A Ghaderi*
Affiliation:
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Iran
*
Address for correspondence: Dr Abbas Ghaderi, Professor of Immunology, Shiraz Institute for Cancer Research, PO Box 71345-3119, Shiraz, Iran. Fax: 0098 711 2304952 E-mail: [email protected]
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Abstract

Objective:

To investigate the association of two functional single nucleotide polymorphisms in the promoter region of the interleukin-18 gene, at positions −607 and −137, with head and neck squamous cell carcinoma.

Design:

Genomic deoxyribonucleic acid was extracted, by the salting-out method, from peripheral blood leukocytes. Single nucleotide polymorphisms of the interleukin-18 gene at positions −607 (cytosine/adenine) and −137 (guanine/cytosine) were analysed by sequence-specific polymerase chain reaction.

Subjects:

One hundred and eleven patients (86 men and 25 women; mean age 56.7±13.7 years) and 212 regional controls (165 men and 47 women; mean age 53.3±12.2 years) were studied. Control subjects comprised healthy volunteers or cancer-free individuals presenting with otolaryngological disease. The diagnosis of squamous cell carcinoma was confirmed histopathologically. Various clinical parameters were collected at diagnosis, including tumour site, tumour size, lymph node involvement, distant metastasis and stage.

Results:

There was no significant association between the allele, genotype or haplotype frequencies of the two single nucleotide polymorphisms of the interleukin-18 promoter and the head and neck squamous cell carcinoma susceptibility or clinical parameters at diagnosis.

Conclusion:

Interleukin-18 polymorphisms at positions −607 and −137 did not confer susceptibility to head and neck squamous cell carcinoma in southern Iranian patients.

Keywords

Squamous Cell CarcinomaInterleukin-18PolymorphismHead and Neck Neoplasms
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 123 , Issue 4 , April 2009 , pp. 444 - 448
Copyright
Copyright © JLO (1984) Limited 2008

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