Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-28T02:25:59.604Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of caspase-3 and caspase-8 deregulation in tongue squamous cell carcinoma, based on immunohistochemistry and computerised image analysis

Published online by Cambridge University Press:  23 May 2008

D Andressakis ,
A C Lazaris ,
E Tsiambas ,
N Kavantzas ,
A Rapidis  and
E Patsouris
D Andressakis
Affiliation:
Department of Oral and Maxillofacial Surgery, St Savas Anticancer Hospital, Athens, Greece
A C Lazaris
Affiliation:
Department of Pathology, Medical School, National & Kapodistrian University, Athens, Greece
E Tsiambas*
Affiliation:
Department of Immunohistochemistry, 401GA Hospital, Athens, Greece
N Kavantzas
Affiliation:
Department of Pathology, Medical School, National & Kapodistrian University, Athens, Greece
A Rapidis
Affiliation:
Department of Oral and Maxillofacial Surgery, St Savas Anticancer Hospital, Athens, Greece
E Patsouris
Affiliation:
Department of Pathology, Medical School, National & Kapodistrian University, Athens, Greece
*
Address for correspondence: Dr E Tsiambas, SOA No 20, Vathi-Samos, Greece83100. Fax: 00302273027947, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Aims:

To investigate the potential role of caspase-3 and caspase-8 protein expression in the biological behaviour of tongue squamous cell carcinoma.

Materials and methods:

We conducted immunohistochemical analyses of 87 specimens of primary tongue squamous cell carcinoma, using monoclonal anti-caspase-3 and anti-caspase-8 antibodies. A digital image analysis assay was also performed in order to evaluate the results.

Results:

Reduced expression of caspase-8 and -3 proteins was observed in 30/87 (34.5 per cent) and 79/87 (90.5 per cent) cases, respectively. Cox regression analysis showed no prognostic significance for the association between overall protein expression of either marker and survival probability (p = 0.174 for caspase-3; p = 0.608 for caspase-8). Interestingly, the size of the examined tumours was strongly correlated with survival status (p = 0.024).

Conclusions:

Simultaneous deregulation of caspase-8 and -3 is a frequent event in tongue squamous cell carcinoma. Activation of caspase-3, which is predominantly down-regulated, may be a crucial process for induction of apoptosis and response to therapeutic strategies.

Keywords

CaspasesApoptosisTongueSquamous Cell CarcinomaImage Analysis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 11 , November 2008 , pp. 1213 - 1218
Copyright
Copyright © JLO (1984) Limited 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Greenlee, RT, Murray, T, Bolden, S, Wingo, PA. Cancer statistics, 2000. CA Cancer J Clin 2000;50:7–33CrossRefGoogle ScholarPubMed
2 Vokes, EE, Weichselbaum, RR, Lippman, SM, Hong, WK. Head and neck cancer. N Engl J Med 1993;328:184–94CrossRefGoogle ScholarPubMed
3 Do, KA, Johnson, MM, Lee, JJ, Wu, XF, Dong, Q, Hong, WK et al. Longitudinal study of smoking-related secondary primary tumours in patients with upper aero-digestive tract malignancies. Cancer 2004;101:2837–42Google Scholar
4 Viswanathan, H, Wilson, JA. Alcohol – the neglected risk factor in head and neck cancer. Clin Otolaryngol 2004;29:295–300CrossRefGoogle ScholarPubMed
5 Veltman, JA, Bot, FJ, Huynen, FC, Ramaekers, FC, Manni, JJ, Hopman, AH. Chromosome instability as an indicator of malignant progression in laryngeal mucosa. J Clin Oncol 2000;18:1644–51CrossRefGoogle ScholarPubMed
6 Maellaro, E, Pacenti, L, Del Bello, B, Valentini, MA, Mangiavacchi, P, De Felice, C et al. Different effects of interferon-alpha on melanoma cell lines: a study on telomerase reverse transcriptase, telomerase activity and apoptosis. Br J Dermatol 2003;148:1115–24CrossRefGoogle Scholar
7 Goldkorn, A, Blackburn, EH. Assembly of mutant-template telomerase RNA into catalytically active telomerase ribonucleoprotein that can act on telomeres is required for apoptosis and cell cycle arrest in human cancer cells. Cancer Res 2006;66:5763–71Google Scholar
8 Rhee, JC, Khuri, FR, Shin, DM. Advances in chemoprevention of head and neck cancer. Oncologist 2004;9:302–11Google Scholar
9 Zhang, P, Zhang, Z, Zhou, X, Qiu, W, Chen, F, Chen, W. Identification of genes associated with cisplatin resistance in human oral squamous cell carcinoma cell line. BMC Cancer 2006;6:224–7CrossRefGoogle ScholarPubMed
10 Fadeel, B, Orrenius, S. Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease. J Int Med 2005;258:479–517CrossRefGoogle ScholarPubMed
11 Patel, T, Gores, GJ, Kaufmann, SH. The critical role of proteases during apoptosis. FASEB J 1996;10:587–97CrossRefGoogle Scholar
12 Medema, JP, Scaffidi, C, Kischel, FC. FLICE is activated by association with the CD 95 death-inducing signaling complex (DISC). EMBO J 1997;16:2794–804Google Scholar
13 Samali, A, Zhivotovsky, B, Jones, D, Nagata, S, Orrenius, S. Apoptosis: cell death defined by caspase activation. Cell Death Differ 1999;6:495–6CrossRefGoogle ScholarPubMed
14 Okada, H, Mak, TW. Pathways of apoptotic and non-apoptotic death in tumour cells. Nat Rev Cancer 2004;4:592–603Google Scholar
15 Vakkala, M, Pääkkö, P, Soini, Y. Expression of caspases 3, 6 and 8 is increased in parallel with apoptosis and histological aggressiveness of the breast lesion. Br J Cancer 1999;81:592–9CrossRefGoogle Scholar
16 Muzio, M, Stockwell, BR, Stennicke, HR, Salvesen, GS, Dixit, VM. An induced proximity model for caspase-8 activation. J Biol Chem 1998;273:2926–30CrossRefGoogle ScholarPubMed
17 Chou, K-C, Jones, D, Heinrikson, RL. Prediction of the tertiary structure and substrate binding site of caspase-8. FEBS Lett 1997;419:49–54Google Scholar
18 de Vicente, JC, Olay, S, Lequerica-Fernandez, P, Sanchez-Mayoral, J, Junquera, LM, Fresno, MF. Expression of Bcl-2 but not Bax has a prognostic significance in tongue carcinoma. J Oral Pathol Med 2006;35:140–5Google Scholar
19 Zhang, YX, Yu, SB, Ou-Yang, JP, Xia, D, Wang, M, Li, JR. Effect of protein kinase C alpha, caspase-3, and survivin on apoptosis of oral cancer cells induced by staurosporine. Acta Pharmacol Sin 2005;26:1365–72Google Scholar
20 Kozaki, K, Imoto, I, Pimkhaokham, A, Hasegawa, S, Tsuda, H, Omura, K et al. PIK3CA mutation is an oncogenic aberration at advanced stages of oral squamous cell carcinoma. Cancer Sci 2006;97:1351–8CrossRefGoogle ScholarPubMed
21 Niu, XW, Feng, J, Peng, ZH, Ma, HQ, Liu, C, Yuan, JY. Receptor-related mechanism of proliferation inhibition and apoptosis induction of human tongue squamous cell line Tca8113 by retinoids [In Chinese]. Di Yi Jun Yi Da Xue Xue Bao 2005;25:935–41Google Scholar
22 Ohtani, T, Hatori, M, Ito, H, Takizawa, K, Kamijo, R, Nagumo, M. Involvement of caspases in 5-FU induced apoptosis in an oral cancer cell line. Anticancer Res 2000;20:3117–21Google Scholar
23 Guo, J, Zhang, ZY. Investigation of apoptosis mechanism of arsenic trioxide on oral squamous cell carcinoma [In Chinese]. Zhonghua Kou Qiang Yi Xue Za Zhi 2003;38:20–3Google ScholarPubMed
24 Sharma, H, Sen, S, Lo Muzio, L, Mariggio, A, Singh, N. Antisense-mediated downregulation of anti-apoptotic proteins induces apoptosis and sensitizes head and neck squamous cell carcinoma cells to chemotherapy. Cancer Biol Ther 2005;4:720–7Google Scholar
25 Pignon, JP, Bourhis, J, Domenge, C. Chemotherapy added to locoregional treatment for head and neck squamous-cell carcinoma: three meta-analyses of up-dated individual data. Lancet 2000;355:949–55CrossRefGoogle Scholar
26 Vokes, EE, Stenson, K, Rosen, FR. Weekly carboplatin and paclitaxel followed by concomitant paclitaxel, fluorouracil, and hydroxyurea chemoradiotherapy: curative and organ-preserving therapy for advanced head and neck cancer. J Clin Oncol 2003;21:320–6Google Scholar
27 Babich, H, Zuckerbraun, HL, Weinerman, SM. In vitro cytotoxicity of (-)-catechin gallate, a minor polyphenol in green tea. Toxicol Lett 2007;171(3):171–80Google Scholar
28 Mohan, KV, Gunasekaran, P, Varalakshmi, E, Hara, Y, Nagini, S. In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells. Cell Biol Int 2007;31:599–608CrossRefGoogle ScholarPubMed
29 Yasumoto, J, Kirita, T, Takahashi, A, Ohnishi, K, Imai, Y, Yuki, K et al. Apoptosis-related gene expression after hyperthermia in human tongue squamous cell carcinoma cells harboring wild-type or mutated-type p53. Cancer Lett 2004;204:41–51CrossRefGoogle ScholarPubMed
30 Yasumoto, J, Imai, Y, Takahashi, A, Ohnishi, K, Yuki, K, Kirita, T et al. Analysis of apoptosis-related gene expression after X-ray irradiation in human tongue squamous cell carcinoma cells harboring wild-type or mutated p53 gene. J Radiat Res 2003;44:41–5Google Scholar