Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-28T05:15:12.406Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of vancomycin-coated tympanostomy tubes on methicillin-resistant Staphylococcus aureus biofilm formation: in vitro study

Published online by Cambridge University Press:  08 January 2010

C H Jang ,
H Park ,
Y B Cho  and
C H Choi
C H Jang*
Affiliation:
Department of Otolaryngology, Chonnam National University Medical School, South Korea Department of Otolaryngology, Research Center for Resistant Cells, Chosun University, Gwangju, South Korea
H Park
Affiliation:
Department of Otolaryngology, Research Center for Resistant Cells, Chosun University, Gwangju, South Korea
Y B Cho
Affiliation:
Department of Otolaryngology, Chonnam National University Medical School, South Korea
C H Choi
Affiliation:
Department of Otolaryngology, Research Center for Resistant Cells, Chosun University, Gwangju, South Korea
*
Address for correspondence: Dr Chul Ho Jang, Department of Otolaryngology, Chonnam National University Medical School, Hakdong 8, Dongku, South Korea. Fax: +82 62 2206776 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Background and objective:

Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. It has been suggested that the tube material may be an important factor in the persistence of such otorrhoea. Development of methicillin-resistant Staphylococcus aureus otorrhoea after tympanostomy tube placement is a growing concern. We evaluated the effect of using vancomycin and chitosan coated tympanostomy tubes on the incidence of methicillin-resistant Staphylococcus aureus biofilm formation in vitro.

Materials and methods:

Three sets each of vancomycin-coated silicone tubes (n = 5), commercial silver oxide coated silicone tubes (n = 5) and uncoated tympanostomy tubes (as controls; n = 5) were compared as regards resistance to methicillin-resistant Staphylococcus aureus biofilm formation after in vitro incubation.

Results:

Scanning electron microscopy showed that the surfaces of the silver oxide coated tubes supported the formation of thick biofilms with crusts, comparable to the appearance of the uncoated tubes. In contrast, the surface of the vancomycin-coated tympanostomy tubes was virtually devoid of methicillin-resistant Staphylococcus aureus biofilm.

Conclusion:

Vancomycin-coated tympanostomy tubes resist methicillin-resistant Staphylococcus aureus biofilm formation. Pending further study, such tubes show promise in assisting the control of methicillin-resistant Staphylococcus aureus biofilm formation.

Keywords

Middle EarVentilation TubesOtorrheaMethicillin-Resistant Staphylococcus Aureus
Type
Review Articles
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 6 , June 2010 , pp. 594 - 598
Copyright
Copyright © JLO (1984) Limited 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Barfoed, C, Rosborg, J. Secretory otitis media. Arch Otolaryngol 1980;106:553–6CrossRefGoogle ScholarPubMed
2 Balknay, TH, Barkin, RM, Suzuki, BH, Watson, WJ. A prospective study of infection following tympanostomy and tube insertion. Am J Otol 1983;4:288–91Google Scholar
3 Baker, RS, Chole, RA. A randomized clinical trial of topical gentamicin after tympanostomy tube placement. Arch Otolaryngol Head Neck Surg 1988;114:755–7CrossRefGoogle ScholarPubMed
4 Giebink, SG, Daly, K, Buran, DJ, Satz, M, Ayre, T. Predictors for postoperative otorrhea following tympanostomy tube insertion. Arch Otolaryngol Head Neck Surg 1992;118:491–4CrossRefGoogle ScholarPubMed
5 Younis, RT, Lazar, RH, Long, TE. Ventilation tubes and prophylactic antibiotic eardrops. Otolaryngol Head Neck Surg 1992;106:193–5CrossRefGoogle ScholarPubMed
6 Bluestone, CD, Klein, JO. Otitis Media in Infants and Children, Chapter 8, 3rd edn. Philadelphia: WB Saunders, 2001;1–15Google Scholar
7 Jang, CH, Song, CH, Wang, PC. Topical vancomycin for chronic suppurative otitis media with methicillin-resistant Staphylococcus aureus otorrhoea. J Laryngol Otol 2004;118:645–7CrossRefGoogle ScholarPubMed
8 AI-Shawwa, BA, Wegner, D. Trimethoprim-sulfamethoxazole plus topical antibiotics as therapy for acute otitis media with otorrhea caused by community-acquired methicillin-resistant Staphylococcus aureus in children. Arch Otolaryngol Head Neck Surg 2005;131:782–4CrossRefGoogle Scholar
9 Coticchia, JM, Dohar, JE. Methicillin-resistant Staphylococcus aureus otorrhea after tympanostomy tube placement. Arch Otolaryngol Head Neck Surg 2005;131:868–73CrossRefGoogle ScholarPubMed
10 Karlan, MS, Mufson, RA, Grizzard, MB, Buscemi, PA, Hench, L, Goldberg, EP. Myringotomy tube materials: bacterial adhesion and infection. Otolaryngol Head Neck Surg 1980;88:783–95CrossRefGoogle ScholarPubMed
11 Jang, CH, Cho, YB, Choi, CH. Structural features of tympanostomy tube biofilm formation in ciprofloxacin-resistant Pseudomonas otorrhea. Int J Pediatr Otorhinolaryngol 2007;71:591–5CrossRefGoogle ScholarPubMed
12 Johnson, JR, Roberts, PL, Olsen, RJ, Moyer, KA, Stamin, WE. Prevention of catheter-associated urinary tract infection with a silver oxide-coated urinary catheter. Clinical and microbilogic correlates. J Infect Dis 1990;162:1145–50CrossRefGoogle Scholar
13 Raad, I, Darouiche, R, Hachem, R, Abi-Said, D, Safar, H, Darnule, T et al. Antimicrobial durability and rare ultrastructural colonization of indwelling central catheters coated with minocycline and rifampin. Crit Care Med 1998;26:219–24CrossRefGoogle ScholarPubMed
14 Donlan, RM, Costerton, JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 2002;15:167–93CrossRefGoogle ScholarPubMed
15 Chole, RA, Hubbell, RN. Antimicrobial activity of silastic tympanostomy tubes impregnated with silver oxide. Arch Otolaryngol Head Neck Surg 1995;121:562–5CrossRefGoogle ScholarPubMed
16 Jang, CH, Park, H, Cho, YB, Choi, CH, Park, IY. The use of piperacillin-tazobactam coated tympanostomy tubes against ciprofloxacin-resistant Pseudomonas biofilm formation: an in vitro study. Int J Pediatr Otorhinolaryngol 2009;73:295–9CrossRefGoogle ScholarPubMed
17 Brummett, RE. Ototoxicity of vancomycin and analogues. Otol Clin 1993;26:821–8Google ScholarPubMed
18 Mellor, JA, Kingdom, J, Cafferkey, M, Keane, C. Vancomycin ototoxicity: a prospective study. J Antimicrob Chemother 1985;15:773–80CrossRefGoogle Scholar
19 Aimin, C, Chunlin, H, Juliang, B, Tinyin, Z, Zhichao, D. Antibiotic loaded chitosan bar: an in vitro, in vivo study of a possible treatment for osteomyelitis. Clin Orthop Relat Res 1999;366:239–47CrossRefGoogle Scholar
20 Yuan, XB, Yuan, YB, Jiang, W, Liu, J, Tian, EJ, Shun, HM et al. Preparation of rapamycin-loaded chitosan/PLA nanoparticles for immunosuppression in corneal transplantation. Int J Pharm 2008;349:241–8CrossRefGoogle ScholarPubMed
21 Bumgardner, JD, Wiser, R, Gerard, PD, Bergin, P, Chesnutt, B, Marin, M et al. Chitosan: potential use as a bioactive coating for orthopaedic and craniofacial/dental implants. J Biomater Sci Polym Ed 2003;14:423–38CrossRefGoogle ScholarPubMed
22 Campbell, AA, Song, L, Li, XS, Nelson, BJ, Bottoni, C, Brooks, DE et al. Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization. J Biomed Mater Res 2000;53:400–73.0.CO;2-Z>CrossRefGoogle ScholarPubMed
23 Hamilton, V, Yuan, Y, Rigney, DA, Chesnutt, BM, Puckett, AD, Ong, JL et al. Bone cell attachment and growth on well-characterized chitosan films. Polymer International 2007;56:641–7CrossRefGoogle Scholar
24 Biedlingmaier, JF, Samaranayake, R, Whelan, P. Resistance to biofilm formation on otologic implant materials. Otolaryngol Head Neck Surg 1998;118:444–51Google ScholarPubMed
25 Saidi, IS, Biedlingmaier, JF, Whelan, P. In vivo resistance to bacterial biofilm formation on tympanostomy tubes as a function of tube material. Otolaryngol Head Neck Surg 1999;120:621–7CrossRefGoogle ScholarPubMed
26 Licameli, G, Johnston, P, Luz, J, Daley, J, Kenna, M. Phosphorylcholine-coated antibiotic tympanostomy tubes: are post-tube placement complications reduced? Int J Pediatr Otorhinolaryngol 2008;72:1323–8CrossRefGoogle ScholarPubMed
27 Cağavi, F, Akalan, N, Celik, H, Gür, D, Güçiz, B. Effect of hydrophilic coating on microorganism colonization in silicone tubing. Acta Neurochir (Wien) 2004;146:603–10CrossRefGoogle ScholarPubMed