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Betadine has a ciliotoxic effect on ciliated human respiratory cells

Published online by Cambridge University Press:  03 November 2014

J H Kim ,
J Rimmer ,
N Mrad ,
S Ahmadzada  and
R J Harvey
J H Kim*
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia
J Rimmer
Affiliation:
St. Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia Woolcock Institute of Medical Research, University of Sydney, Glebe, New South Wales, Australia
N Mrad
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia
S Ahmadzada
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia Department of Medicine, University of Notre Dame Australia, Darlinghurst, New South Wales, Australia
R J Harvey
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia St. Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia The Australian School of Advanced Medicine, Macquarie University, Macquarie Park, New South Wales, Australia
*
Address for correspondence: Miss Joo Hye Kim, Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research (AMR), Level 8 Lowy Packer Building – St. Vincent's Research Precinct, 405 Liverpool Street, Darlinghurst, New South Wales 2010, Australia E-mail: [email protected]
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Abstract

Objective:

This study investigated the effect of Betadine on ciliated human respiratory epithelial cells.

Methods:

Epithelial cells from human sinonasal mucosa were cultured at the air–liquid interface. The cultures were tested with Hanks' balanced salt solution containing 10 mM HEPES (control), 100 µM ATP (positive control), 5 per cent Betadine or 10 per cent Betadine (clinical dose). Ciliary beat frequency was analysed using a high-speed camera on a computer imaging system.

Results:

Undiluted 10 per cent Betadine (n = 6) decreased the proportion of actively beating cilia over 1 minute (p < 0.01). Ciliary beat frequency decreased from 11.15 ± 4.64 Hz to no detectable activity. The result was similar with 5 per cent Betadine (n = 7), with no significant difference compared with the 10 per cent solution findings.

Conclusion:

Betadine, at either 5 and 10 per cent, was ciliotoxic. Caution should be applied to the use of topical Betadine solution on the respiratory mucosal surface.

Keywords

BetadinePovidone-IodineCiliary Beat FrequencyAir Liquid InterfaceRespiratory EpitheliumPrimary Cell CultureCilia
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 129 , Issue S1 , January 2015 , pp. S45 - S50
Copyright
Copyright © JLO (1984) Limited 2014 

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References

1Chin, D, Harvey, RJ. Nasal polyposis: an inflammatory condition requiring effective anti-inflammatory treatment. Curr Opin Otolaryngol Head Neck Surg 2013;21:23–30CrossRefGoogle ScholarPubMed
2Rudmik, L, Hoy, M, Schlosser, RJ, Harvey, RJ, Welch, KC, Lund, V et al. Topical therapies in the management of chronic rhinosinusitis: an evidence-based review with recommendations. Int Forum Allergy Rhinol 2013;3:281–98CrossRefGoogle ScholarPubMed
3Harvey, RJ, Psaltis, A, Schlosser, RJ, Witterick, IJ. Current concepts in topical therapy for chronic sino-nasal disease. J Otolaryngol Head Neck Surg 2009;39:217–31Google Scholar
4Thomas, WW, Harvey, RJ, Rudmik, L, Hwang, PH, Schlosser, RJ. Distribution of topical agents to the paranasal sinuses: an evidence-based review with recommendations. Int Forum Allergy Rhinol 2013;3:691–703CrossRefGoogle Scholar
5Raza, T, Elsherif, HS, Zulianello, L, Plouin-Gaudon, I, Landis, BN, Lacroix, JS. Nasal lavage with sodium hypochlorite solution in Staphylococcus aureus persistent rhinosinusitis. Rhinology 2008;46:15–22Google Scholar
6Rombaux, P, Collet, S, Hamoir, M, Eloy, P, Bertrand, B, Jamart, F et al. The role of nasal cavity disinfection in the bacteriology of chronic sinusitis. Rhinology 2005;43:125–9Google Scholar
7Gottardi, W. Iodine and iodine compounds. In: Block, SS, ed. Disinfection, Sterilization and Preservation. Philadelphia: Lea & Febiger, 1983;183–96Google Scholar
8Gosepath, J, Grebneva, N, Mossikhin, S, Mann, WJ. Topical antibiotic, antifungal, and antiseptic solutions decrease ciliary activity in nasal respiratory cells. Am J Rhinol 2000;14:411–18Google Scholar
9LeSimple, P, van Seuningen, I, Buisine, MP, Copin, MC, Hinz, M, Hoffmann, W et al. Trefoil factor family 3 peptide promotes human airway epithelial ciliated cell differentiation. Am J Respir Cell Mol Biol 2007;36:296–303CrossRefGoogle ScholarPubMed
10Dimova, S, Brewster, ME, Noppe, M, Jorissen, M, Augustijns, P. The use of human nasal in vitro cell systems during drug discovery and development. Toxicol In Vitro 2005;19:107–22CrossRefGoogle ScholarPubMed
11Antunes, MB, Woodworth, BA, Bhargave, G, Xiong, G, Aguilar, JL, Ratner, AJ et al. Murine nasal septa for respiratory epithelial air-liquid interface cultures. Biotechniques 2007;43:195–204CrossRefGoogle ScholarPubMed
12Woodworth, B, Zhang, S, Tamashiro, E, Bhargave, G, Palmer, J, Cohen, NA. Zinc increases ciliary beat frequency in a calcium-dependent manner. Am J Rhinol Allergy 2010;24:6–10Google Scholar
13Shen, JC, Cope, E, Chen, B, Leid, JG, Cohen, NA. Regulation of murine sinonasal cilia function by microbial secreted factors. Int Forum Allergy Rhinol 2012;2:104–10Google Scholar
14Green, A, Smallman, LA, Logan, ACM, Drake-Lee, AB. The effect of temperature on nasal ciliary beat frequency. Clin Otolaryngol 1995;20:178–80CrossRefGoogle ScholarPubMed
15Lieb, T, Frei, CW, Frohock, JI, Bookman, RJ, Salathe, M. Prolonged increase in ciliary beat frequency after short-term purinergic stimulation in human airway epithelial cells. J Physiol 2002;538:633–46CrossRefGoogle ScholarPubMed