Hostname: page-component-cc8bf7c57-n7pht Total loading time: 0 Render date: 2024-12-12T02:00:57.020Z Has data issue: false hasContentIssue false
  • English
  • Français

Uptake and utilization of arachidonic acid in infective larvae of Angiostrongylus cantonensis

Published online by Cambridge University Press:  25 November 2010

P. Tang  and
L.-C. Wang
P. Tang
Affiliation:
Department of Parasitology, College of Medicine, Chang Gung University, Tao-Yuan33332, Taiwan
L.-C. Wang*
Affiliation:
Department of Parasitology, College of Medicine, Chang Gung University, Tao-Yuan33332, Taiwan
*
*E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Infective larvae of Angiostrongylus cantonensis may take up and incorporate exogenous arachidonic acid into their lipid pool. By scintillation counting, uptake and incorporation were determined to be time dependent. Arachidonic acid was mainly incorporated into phospholipid (56.8%) and neutral lipid (22.4%) pools. In the neutral lipids, 64.0% was diglyceride and 36.0% triglyceride. Phosphatidylcholine was the predominant fatty acid in the phospholipid pool. In addition to the release of leukotriene B4, the parasite was found to generate radiolabelled CO2 after incubation with [U-14C]arachidonate. Moreover, enzymatic analysis of crude extracts revealed the presence of acyl-CoA dehydrogenase (short and long chain), thiolase, enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase. These findings suggest that infective larvae of A. cantonensis not only take up and incorporate exogenous arachidonic acid into their lipid pool, but may also utilize the fatty acid through a functional β-oxidation pathway.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 4 , December 2011 , pp. 395 - 400
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdel Baset, H., O'Neill, G.P. & Ford-Hutchinson, A.W. (1995) Characterization of arachidonic-acid-metabolizing enzymes in adult Schistosoma mansoni. Molecular and Biochemical Parasitology 73, 31–41.CrossRefGoogle Scholar
Alicata, J.E. (1965) Biology and distribution of the rat lungworm, Angiostrongylus cantonensis, and its relationship to eosinophilic meningoencephalitis and other neurological disorders of man and animals. Advances in Parasitology 3, 223–248.Google Scholar
Barakat, H., Brown, W. & Henry, S.D. (1978) Studies of fatty acid oxidation in homogenates of the cardiomyopathic hamster. Life Science 23, 1835–1840.CrossRefGoogle ScholarPubMed
Clancy, R.M. & Hugli, T.E. (1983) The extraction of leukotrienes (LTC4, LTD4, and LTE4) from tissue fluids: the metabolism of these mediators during IgE-dependent hypersensitivity reactions in lung. Analytical Biochemistry 133, 30–39.CrossRefGoogle Scholar
Daugschies, A. & Joachim, A. (2000) Eicosanoids in parasites and parasitic infections. Advances in Parasitology 46, 181–240.CrossRefGoogle ScholarPubMed
Fong, J.C. & Schulz, H. (1978) On the rate-determining step of fatty acid oxidation in heart. Inhibition of fatty acid oxidation by 4-pentenoic acid. Journal of Biological Chemistry 253, 6917–6922.Google Scholar
Frayha, G.J. & Smyth, J.D. (1983) Lipid metabolism in parasitic helminths. Advances in Parasitology 22, 309–387.CrossRefGoogle ScholarPubMed
Fusco, A.C., Salafsky, B. & Kevin, M.B. (1985) Schistosoma mansoni: eicosanoid production by cercariae. Experimental Parasitology 59, 44–50.CrossRefGoogle ScholarPubMed
Fusco, A.C., Salafsky, B. & Delbrook, K. (1986) Schistosoma mansoni: production of cercarial eicosanoids as correlates of penetration and transformation. Journal of Parasitology 72, 397–404.Google Scholar
Golan, D.E., Brown, C.S., Cianci, C.M., Furlong, S.T. & Caulfield, J.P. (1986) Schistosomula of Schistosoma mansoni use lysophosphatidylcholine to lyse adherent human red blood cells and immobilize red cell membrane components. Journal of Cell Biology 103, 819–828.Google Scholar
Hall, C.L. (1981) Acyl-CoA dehydrogenases from pig liver mitochondria. Methods in Enzymology 71, 375–385.Google Scholar
Harizi, H., Corcuff, J.B. & Gualde, N. (2008) Arachidonic-acid-derived eicosanoids: roles in biology and immunopathology. Trends of Molecular Medicine 14, 461–469.CrossRefGoogle ScholarPubMed
Lazarow, P.B. & de Duve, D. (1976) A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug. Proceedings of the National Academy of Sciences of the United States of America 73, 2043–2046.CrossRefGoogle ScholarPubMed
Leid, R.W. & McConnell, L.A. (1983a) Thromboxane A2 generation by the larval cestode, Taenia taeniaeformis. Clinical Immunology and Immunopathology 28, 67–76.CrossRefGoogle ScholarPubMed
Leid, R.W. & McConnell, L.A. (1983b) PGE2 generation and release by the larval stage of the cestode, Taenia taeniaeformis. Prostaglandins, Leukotrienes, and Medicine 11, 317–323.CrossRefGoogle ScholarPubMed
Liu, L.X. & Weller, P.F. (1990) Arachidonic acid metabolism in filarial parasites. Experimental Parasitology 71, 496–501.Google Scholar
Longworth, D.L., Foster, D.W., Dvorak, A.M. & Weller, P.F. (1985) Incorporation of arachidonic acid by microfilariae of Brugia malayi. Journal of Infectious Diseases 152, 1317–1323.CrossRefGoogle ScholarPubMed
Longworth, D.L., King, D.C. & Weller, P.F. (1987) Rapid uptake and esterification of arachidonic acid and other fatty acids by microfilariae of Brugia malayi. Molecular and Biochemical Parasitology 23, 275–284.CrossRefGoogle ScholarPubMed
Longworth, D.L., Monahan-Earley, R.A., Dvorak, A.M. & Weller, P.F. (1988) Brugia malayi: arachidonic acid uptake into lipid bodies of adult parasites. Experimental Parasitology 65, 251–257.CrossRefGoogle ScholarPubMed
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Nissen, H.P. & Kreysel, H.W. (1983) Analysis of phospholipids in human semen by high-performance liquid chromatography. Journal of Chromatography 276, 29–35.CrossRefGoogle ScholarPubMed
Osmunden, H. (1981) Spectrophotometric procedure for measuring mitochondrial beta-oxidation. Methods in Enzymology 72, 306–314.CrossRefGoogle Scholar
Pelech, S.L. & Vance, D.E. (1984) Regulation of phosphatidylcholine biosynthesis. Biochimica et Biophysica Acta 799, 217–251.CrossRefGoogle Scholar
Pilitsis, J.G., Diaz, F.G., Wellwood, J.M., Oregan, M.H., Fairfax, M.R., Phillis, J.W. & Coplin, W.M. (2001) Quantification of free fatty acids in human cerebrospinal fluid. Neurochemical Research 26, 1265–1270.CrossRefGoogle ScholarPubMed
Potter, K. & Leid, R.W. (1986) A review of eosinophil chemotaxis and function in Taenia taeniaeformis infections in the laboratory rat. Veterinary Parasitology 20, 103–116.Google Scholar
Reichmann, H., Pette, D. & Vrbová, G. (1983) Electrostimulation-induced changes in the enzyme activity pattern of dystrophic mouse muscles. Acta Histochemica (Suppl.) 28, 125–127.Google ScholarPubMed
Rumjanek, F.D., McLaren, D.J. & Smithers, S.R. (1983) Serum-induced expression of a surface protein in schistosomula of Schistosoma mansoni: a possible receptor for lipid uptake. Molecular and Biochemical Parasitology 9, 337–350.Google Scholar
Samuelsson, B. (1983) Leukotrienes: mediators of immediate hypersensitivity reactions and inflammation. Science 220, 568–575.Google Scholar
Shih, H.H. & Chen, S.N. (1982) Glycolytic enzymes in juvenile and adult Angiostrongylus cantonensis. The Southeast Asian Journal of Tropical Medicine and Public Health 13, 114–119.Google Scholar
Srivastava, A.K. & Jaffe, J.J. (1985) Phosphatidylcholine synthesis in adult Dirofilaria immitis females. International Journal for Parasitology 15, 27–31.Google Scholar
Thorpe, C. (1981) Acyl-CoA dehydrogenase from pig kidney. Methods in Enzymology 71, 366–374.Google Scholar
Wakil, S.J. & Mahler, H.R. (1954) Studies on the fatty acid oxidizing system of animal tissues. V. Unsaturated fatty acyl coenzyme A hydrase. Journal of Biological Chemistry 207, 125–132.CrossRefGoogle ScholarPubMed
Wang, L.C., Chao, D. & Chen, E.R. (1989) Acquired immunity in rats against Angiostrongylus cantonensis infection. International Journal for Parasitology 19, 617–620.Google ScholarPubMed
Weller, P.F., Longworth, D.L. & Jaffe, J.J. (1989) Leukotriene C4 synthesis catalyzed by Dirofilaria immitis glutathione S-transferase. American Journal of Tropical Medicine and Hygiene 40, 171–175.Google Scholar