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Transformation of cercariae to schistosomula: A quantitative comparison of transformation techniques and of infectivity by different injection routes of the organisms produced

Published online by Cambridge University Press:  05 June 2009

E. R. James  and
M. G. Taylor
E. R. James
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, St. Albans, Hertfordshire AIA 0XQ, England
M. G. Taylor
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, St. Albans, Hertfordshire AIA 0XQ, England
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Abstract

Six transformation techniques ((i) incubation of cercariae in isotonic; medium; (ii) penetration of isolated skin; (iii) stimulation with crude egg lecithin; (iy) mechanical disruption by passage through a syringe and 21 gauge needle; (v) by whorling of cercariae with a vortex mixer; (vi) syringe transformation followed by incubation) were evaluated. The organisms produced were assessed for infectivity for mice by 6 different routes of infection (percutaneous, intradermal, subcutaneous, intra-peritoneal, intravenous and intramuscular). At perfusion the largest numbers of adult worms were recovered from mice injected intramuscularly with organisms produced by the syringe technique and control mice given cercariae percutaneously. The peak for migration of schistosomula (following syringe transformation and intramuscular injection) through the lungs and the appearance of adult worms in the mesenteries was shown to be similar to cercariae administered percutaneously.

Type
Research Article
Information
Journal of Helminthology , Volume 50 , Issue 4 , December 1976 , pp. 223 - 233
Copyright
Copyright © Cambridge University Press 1976

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References

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