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Therapeutic efficacy of a newly synthesized benzimidazole compound BTP-OH against murine schistosomiasis mansoni

Published online by Cambridge University Press:  15 July 2020

A. Taman Open the ORCID record for A. Taman [Opens in a new window] ,
B. Mansour ,
M.Y. Youssef  and
S.M. Alhusseiny
A. Taman*
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Mansoura University, 2 El-Gomhouria Street, Mansoura35516, Egypt
B. Mansour
Affiliation:
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa City, Mansoura, Egypt
M.Y. Youssef
Affiliation:
Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura35516, Egypt
S.M. Alhusseiny
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Mansoura University, 2 El-Gomhouria Street, Mansoura35516, Egypt
*
Author for correspondence: A. Taman, E-mail: [email protected]
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Abstract

Because of the increasingly emerging praziquantel resistance, there is a crucial need to develop new anti-schistosomal agents. This work was conducted to assess the therapeutic efficacy of a new benzimidazole compound (BTP-OH) in mice experimentally infected with Schistosoma mansoni. A total of 40 Swiss albino female mice were divided into an infected untreated group and three infected treated groups (using praziquantel and BTP-OH). The compound activity was evaluated through parasitological, histopathological and scanning electron microscopy studies. Praziquantel and BTP-OH at both doses significantly reduced male (75%, 42.67% and 61.08%, respectively), female (71.45%, 48.94% and 68.13%, respectively) and total worm burden (75.21%, 42.42% and 62.28%, respectively), as well as tissue egg load in the liver (71.22%, 42.12% and 66.04%, respectively). In oogram, praziquantel significantly increased the percentage of dead eggs (65.89%), while BTP-OH significantly reduced the percentage of immature eggs (30.43% and 19.64%). BTP-OH significantly diminished granuloma count (33.87% and 44.77%) and diameter (39.23% and 49.40%), and caused ultrastructural changes in the tegument of adult schistosomes. This study provides evidence for the schistosomicidal efficacy of BTP-OH. However, future studies are needed to elucidate the full mechanisms of action and effects of BTP-OH on other human schistosomes.

Keywords

Schistosoma mansonimicebenzimidazolesworm burdentissue egg loadoogram patternscanning electron microscopy
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e172
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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