Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-28T04:06:04.439Z Has data issue: false hasContentIssue false
  • English
  • Français

Techniques for locating isotopically labelled schistosomula of Schistosoma mansoni in host tissues for ultrastructural investigations

Published online by Cambridge University Press:  18 November 2009

Jean E. Crabtree  and
R. A. Wilson
Jean E. Crabtree*
Affiliation:
Department of Biology, University of York, York YO1 5DD, UK.
R. A. Wilson
Affiliation:
Department of Biology, University of York, York YO1 5DD, UK.
*
*Department of Medicine, St. James' University Hospital, Leeds, LS9 7TF.
Get access Rights & Permissions [Opens in a new window]

Abstract

The use of 75Selenomethionine labelled cercariae of Schistosoma mansoni for ultrastructural localization of parasites in host tissues has been evaluated. Both gamma counting of tissue, and autoradiography of resin-embedded tissue were successful. The autoradiographic technique was more sensitive and schistosomula were readily located in pulmonary tissue up to 24 days post-infection.

Keywords

SchistosomaMansoniAutoradiographyElectronMicroscopy.
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 1 , March 1986 , pp. 75 - 78
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bentley, A. G., Carlisle, A. S. & Phillips, S. M. (1981) Ultrastructural analysis of the cellular response to Schistosoma mansoni: initial and challenge infections in the rat. American Journal of Tropical Medicine and Hygiene, 30, 102–112.CrossRefGoogle ScholarPubMed
Bruce, J. I., Pezzlo, F., Yajima, Y. & Mccarty, J. E. (1974) Schistosoma Mansoni: Pulmonary Phase of Schistosomule migration studied by electron microscopy. Experimental Parasitology, 35, 150–160.CrossRefGoogle ScholarPubMed
Crabtree, J. E. & Wilson, R. A. (1980) Schistosoma Mansoni: A Scanning Electron Microscope study of the Developing Schistosomulum. Parasitology, 81, 553–564.CrossRefGoogle ScholarPubMed
Crabtree, J. E. & Wilson, R. A. (1984) Schistosoma Mansoni: Cellular Reactions to challenge infections in the cheek pouch skin of chronically infected chinese hamsters. Parasitology, 89, 59–69.CrossRefGoogle ScholarPubMed
Crabtree, J. E. & Wilson, R. A. (1985) Schistosoma Mansoni: an ultrastructural examination of skin migration in the hamster cheek pouch. Parasitology, 91, 111–120.CrossRefGoogle ScholarPubMed
Crabtree, J. E. & Wilson, R. A. (1986) The role of pulmonary cellular reactions in the resistance of vaccinated mice to Schistosoma mansoni. Parasite Immunology, in press.CrossRefGoogle ScholarPubMed
Dean, D. A., Mangold, B. L., Georgi, J. R. & Jacobson, R. H. (1984) Comparison of Schistosoma Mansoni migration patterns in normal and irradiated cercaria immunized mice by means of autoradiography. Evidence that worm elimination occurs after the skin phase in immunized mice. American Journal of Tropical Medicine and Hygiene, 33, 89–96.CrossRefGoogle Scholar
Georgi, J. (1982) Schistosoma mansoni: quantification of skin penetration and early migration by differential external radioassay and autoradiography. Parasitology, 84, 263–281.CrossRefGoogle ScholarPubMed
Mangold, B. L. & Dean, D. A. (1983) Autoradiographic analysis of Schistosoma mansoni migration from skin to lungs in naive mice. Evidence that most attrition occurs after the skin phase. American journal of tropical medicine and hygiene, 32, 785–789.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Coulson, P. R. (1986) Schistosoma Mansoni: dynamics of migration through the vascular system of the mouse. Parasitology, In Press.CrossRefGoogle ScholarPubMed
Wilson, R. A., Coulson, P. S. & Dixon, B. (1986) Migration of the schistosomula of Schistosoma mansoni in mice vaccinated with radiation attenuated cercariae, and normal mice: an attempt to identify the timing and site of parasite death. Parasitology, In Press.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Lawson, J. R. (1980) An Examination of the skin phase of schistosome migration using a hamster cheek pouch preparation. parasitology, 80, 257–266.CrossRefGoogle ScholarPubMed