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A Technique for the Detection of Polystoma integerrimum in the Common Frog (Rana temporaria)

Published online by Cambridge University Press:  18 November 2009

J. B. Jennings
J. B. Jennings
Affiliation:
Department of Zoology, The University of Leeds.
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Extract

An infection of the frog bladder by the Monogenetic Trematode Polystoma integerrimum can be detected by testing the urine for the presence of the pigment haematin. This substance is an end product of the digestion of blood, which the parasite sucks from the capillaries of the bladder wall, and it is voided from the flatworm's gut into the bladder to pass eventually to the exterior with the urine (Jennings—unpublished work).

An alkaline solution of luminol (3-amino pthallic cyclic hydrazide hydrochloride) and hydrogen peroxide exhibits an intense blue luminescence in the presence of haematin (Proescher & Moody, 1938) and this reaction, which is very sensitive, can be used to detect the pigment in the urine of infected frogs.

Type
Research Papers
Information
Journal of Helminthology , Volume 30 , Issue 2-3 , October 1956 , pp. 119 - 120
Copyright
Copyright © Cambridge University Press 1956

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References

Proescher, F. and Moody, A. M., 1938.—“The detection of blood by chemiluminescence.” J. Lab. din. Med., 24, 1183. (W.L. 11285)Google Scholar