Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-27T20:43:58.076Z Has data issue: false hasContentIssue false
  • English
  • Français

A technique for skin application of exact doses of Nippostrongylus brasiliensis to rats

Published online by Cambridge University Press:  05 June 2009

N. R. Tindall  and
P. A. G. Wilson
N. R. Tindall
Affiliation:
University of Edinburgh, Department of Zoology, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
P. A. G. Wilson*
Affiliation:
University of Edinburgh, Department of Zoology, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
*
*Author for correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Conventional methods for percutaneous infection of rats using third-stage juveniles of Nippostrongylus brasiliensis which have been artificially stimulated to exsheath lead to highly variable, and relatively poor, establishment in the intestine. A new system has been developed in which larvae applied to the skin still remain partially sheathed, as they would be in nature. Cultures of the freeliving stages of the parasite contain an annulus of clear, colourless polythene film to which some of the third-stage juveniles attach. Rats are infected with an individually counted, exact dose applied to the skin on polythene. Using this technique (‘EDT20Nb’), consistently high values for the mean proportion of the dose that becomes established have been obtained, along with a variance well below the normally accepted level ( from a total of 73 rats in 12 separate assays). In particular, the added component of variance between assays in this study was insignificant, so that the probability of quantitative agreement in replicate experiments based on the method is high. It is recommended for an experimental design in which small numbers of parasites can be used.

Keywords

Nippostrongylus brasiliensisratskin applicationtechniques
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 1 , March 1990 , pp. 15 - 22
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barakat, M. R. (1951) A new procedure for the cultivation of nematode parasites. Journal of the Egyptian Medical Association, 34, 323–326.Google Scholar
Haley, A. J. (1962a) Biology of the rat nematode, Nippostrongylus brasiliensis (Travassos, 1914): II. Preparasitic stages and development in the laboratory rat. Journal of Parasitology, 48, 13–23.CrossRefGoogle Scholar
Haley, A. J. (1962b) Role of host relationships in the systematics of helminth parasites. Journal of Parasitology, 48, 671–678.CrossRefGoogle Scholar
Ogilvie, B. M. (1965) Use of cortisone derivatives to inhibit resistance to Nippostrongylus brasiliensis and to study the fate of parasites in resistant hosts. Parasitology, 55, 723–730.Google Scholar
Tindall, N. R. & Wilson, P. A. G. (1988) Criteria for a proof of migration routes of immature parasites inside hosts exemplified by studies of Strongyloides ratti in the rat. Parasitology, 96, 551–563.CrossRefGoogle ScholarPubMed
Tindall, N. R. & Wilson, P. A. G. (1990a) A basis to extend the proof of migration routes of immature parasites inside hosts: Estimated time of arrival of Nippostrongylus brasiliensis and Strongyloides ratti in the gut of the rat. Parasitology, (in press).Google ScholarPubMed
Tindall, N. R. & Wilson, P. A. G. (1990b) An extended proof of migration routes of immature parasites inside hosts: Pathways of Nippostrongylus brasiliensis and Strongyloides ratti in the rat are mutually exclusive. Parasitology, (in press).Google ScholarPubMed
Twohy, D. W. (1956) The early migration and growth of Nippostrongylus muris in the rat. American Journal of Hygiene, 63, 165–185.Google Scholar
Wilson, P. A. G. (1965a) Changes in lipid and nitrogen content of Nippostrongylus brasiliensis infective larvae aged at constant temperature. Experimental Parasitology, 16, 190–194.CrossRefGoogle ScholarPubMed
Wilson, P. A. G. (1965b) The effect of temperature change on the oxygen uptake of the infective larvae of Nippostrongylus brasiliensis. Experimental Parasitology, 17, 318–325.Google Scholar
Wilson, P. A. G. (1983) Roundworm juvenile migration in mammals: the pathways of skin-penetrators reconsidered. In: Aspects of Parasitology (editor Meerovitch, E.), pp. 459–85. McGill University Institute of Parasitology, Montreal.Google Scholar
Wilson, P. A. G., Cameron, M. & Scott, D. S. (1978) Strongyloides ratti in virgin female rats: studies of oestrous cycle effects and general variability. Parasitology, 76, 221–227.CrossRefGoogle ScholarPubMed
Wilson, P. A. G. & Dick, J. M. (1964) The culture and isolation of Nippostrongylus brasiliensis infective larvae for biochemical studies. Journal of Helminthology, 38, 399–404.CrossRefGoogle ScholarPubMed
Wilson, P. A. G. & Simpson, N. E. (1981) Dynamics of infection in rats given low doses of homogonic and heterogonic Strongyloides ratti. Parasitology, 83, 459–475.CrossRefGoogle Scholar
Wilson, P. A. G., Simpson, N. E. & Seaton, D. S. (1986) The effect of method of infection on the pathway of juvenile Strongyloides ratti in the host. Journal of Helminthology, 60, 79–91.CrossRefGoogle ScholarPubMed