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Tandem antioxidant enzymes confer synergistic protective responses in experimental filariasis

Published online by Cambridge University Press:  16 May 2013

P.R. Prince ,
J. Madhumathi ,
G. Anugraha ,
P.J. Jeyaprita ,
M.V.R. Reddy  and
P. Kaliraj
P.R. Prince
Affiliation:
Centre for Biotechnology, Anna University, Chennai600025, India
J. Madhumathi
Affiliation:
Centre for Biotechnology, Anna University, Chennai600025, India
G. Anugraha
Affiliation:
Centre for Biotechnology, Anna University, Chennai600025, India
P.J. Jeyaprita
Affiliation:
Centre for Biotechnology, Anna University, Chennai600025, India
M.V.R. Reddy
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram442102, India
P. Kaliraj*
Affiliation:
Centre for Biotechnology, Anna University, Chennai600025, India
*
*Fax:+91 44 22352642, E-mail: [email protected]
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Abstract

Helminth parasites use antioxidant defence strategies for survival during oxidative stress due to free radicals in the host. Accordingly, tissue-dwelling filarial parasites counteract host responses by releasing a number of antioxidants. Targeting these redox regulation proteins together, would facilitate effective parasite clearance. Here, we report the combined effect of protective immune responses trigged by recombinant Wuchereria bancrofti thioredoxin (WbTRX) and thioredoxin peroxidase (WbTPX) in an experimental filarial model. The expression of WbTRX and WbTPX in different stages of the parasite and their cross-reactivity were analysed by enzyme-linked immunosorbent assay (ELISA). The immunogenicity of recombinant proteins and their protective efficacy were studied in animal models when immunized in single or cocktail mode. The antigens showed cross-reactive epitopes and induced high humoral and cellular immune responses in mice. Further, parasite challenge against Brugia malayi L3 larvae in Mastomyscoucha conferred significant protection of 57% and 62% against WbTRX and WbTPX respectively. The efficacy of L3 clearance was significantly higher (71%) (P <  0.001) when the antigens were immunized together, showing a synergistic effect in multiple-mode vaccination. Hence, the study suggests WbTRX and WbTPX to be attractive vaccine candidates when immunized together and provides a tandem block for parasite elimination in the control of lymphatic filariasis.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 4 , December 2014 , pp. 402 - 410
Copyright
Copyright © Cambridge University Press 2013 

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