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Studies on host specificity in Paragonimus westermani: II. Histochemical and cytochemical characterization of metacercariae and worms from rats and dogs

Published online by Cambridge University Press:  05 June 2009

Takahiro Fujino ,
Koichi Fukuda ,
Fusanori Hamajima  and
Yoichi Ishii
Takahiro Fujino
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Koichi Fukuda
Affiliation:
Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan
Fusanori Hamajima
Affiliation:
Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan
Yoichi Ishii
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
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Abstract

Histochemical tests were done on newly excysted metacercariae and worms recovered from an abnormal host (rat) and the definitive host (dog) for some oxidoreductases, phosphatases and glycosidases. The results demonstrate that rat worms have enzymatic distribution and intensities more similar to those of metacercariae than to adult worms from dogs. Ultracytochemical examination of acid and alkaline phosphatase and Mg-ATPase activity was also carried out. Acid phosphatase activity occurred exceptionally in the excretory bladder and caeca of dog worms. No activity was observed in rat worms except for lysosomal granules in the tegument. Alkaline phosphatase activity was exhibited in the excretory bladder in both dog and rat worms. Mg-ATPase activity occurred in the tegument and parenchymal cells in dog worms and in the excretory bladder in rat worms. In metacercariae, little or no reaction for these enzymes was present except for Mg-ATPase activity on the excretory ducts. These observations, together with the histochemical results, indicate that metabolic activity in rat worms is higher than in metacercariae although it is strongly reduced compared with dog worms.

Keywords

Paragonimus westermanihistochemistrycytochemistryhost specificity
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 4 , December 1989 , pp. 315 - 327
Copyright
Copyright © Cambridge University Press 1989

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