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Strongyloides ratti: the role of interleukin-5 in protection against tissue migrating larvae and intestinal adult worms

Published online by Cambridge University Press:  12 April 2024

K. Watanabe ,
O. Sasaki ,
S. Hamano ,
K. Kishihara ,
K. Nomoto ,
I. Tada  and
Y. Aoki
K. Watanabe*
Affiliation:
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
O. Sasaki
Affiliation:
Department of Animal Science, Akita Prefectural College of Agriculture, Akita 010-0444, Japan
S. Hamano
Affiliation:
Department of Microbiology and Immunology (Parasitology), Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
K. Kishihara
Affiliation:
Department of Immunology, Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
K. Nomoto
Affiliation:
Department of Immunology, Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
I. Tada
Affiliation:
Department of Microbiology and Immunology (Parasitology), Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Y. Aoki
Affiliation:
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
*
*Fax: +81 95 849 7805 E-mail: [email protected]
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Abstract

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To determine the role of interleukin-5 (IL-5) and eosinophils in protection against Strongyloides ratti, mice treated with anti-IL-5 monoclonal antibody (mAb) were infected with S. ratti larvae. Strongyloides ratti egg numbers in faeces (EPG) in mAb treated mice were higher than those in control mice on days 6 and 7 after inoculation. The numbers of migrating worms in mAb treated mice 36h after inoculation were higher than those observed in control mice. Intestinal worm numbers in mAb treated mice 5 days after inoculation were higher than those in control mice. These results show that eosinophils effectively protected the host against S. ratti infection by mainly the larval stage in primary infections. The involvement of eosinophils in protection against secondary infection was also examined. Before secondary infection, mice were treated with anti-IL-5 mAb and infected with S. ratti. Patent infections were not observed in either mAb treated or control Ab treated mice. The numbers of migrating worms in the head and lungs of mAb treated mice increased to 60% of that in primary infected mice. Intestinal worms were not found in mAb treated mice or in contcrol mice after oral implantation of adult worms. Eosinophils were therefore mainly involved in protection against tissue migrating worms in secondary infections.

Type
Review Article
Information
Journal of Helminthology , Volume 77 , Issue 4 , December 2003 , pp. 355 - 361
Copyright
Copyright © Cambridge University Press 2003

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