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Stressor-induced changes to the protoscoleces of Echinococcus granulosus of Indian buffalo origin

Published online by Cambridge University Press:  01 December 2008

D. Pan ,
D. Bhattacharya ,
A.K. Bera ,
J. Gudewar ,
S. De  and
S.K. Das
D. Pan
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, India
D. Bhattacharya*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, India
A.K. Bera
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, India
J. Gudewar
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, India
S. De
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, India
S.K. Das
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, India
*
*Fax: +91 3325565725 E-mail: [email protected]
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Abstract

In the present study viable protoscoleces of Echinococcus granulosus were exposed to in vitro anthelmintic treatment to observe efficacy against Indian buffalo isolates. Evaluation criteria included morphological changes, viability scores and expression of peptides as a product of prestressed protoscoleces. Protoscolex changes included presence of bladder-like structure and morphological distortion. Two peptides of relative molecular weight (Mr) 40 and 70 kDa were visualized when proteins were separated by discontinuous gel electrophoresis. These two peptides seemed to be products of prestressed protoscoleces.

Type
Research Note
Information
Journal of Helminthology , Volume 82 , Issue 4 , December 2008 , pp. 309 - 311
Copyright
Copyright © Cambridge University Press 2008

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References

Bhattacharya, D., Bera, A.K., Bera, B.C., Maity, A. & Das, S.K. (2007) Genotypic characterization of Indian cattle, buffalo and sheep isolates of Echinococcus granulosus. Veterinary Parasitology 143, 371–374.CrossRefGoogle ScholarPubMed
Gill, H.S. & Rao, B.V. (1967) On the biology and morphology of Echinococcus granulosus (Batsch, 1786) of buffalo–dog origin. Parasitology 57, 695–704.CrossRefGoogle ScholarPubMed
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biology and Chemistry 93, 265–275.CrossRefGoogle Scholar
Maity, A., Bhattacharya, D., Batabyal, S., Chattopadhyay, S., Bera, A.K. & Karmakar, P.K. (2007) Echinococcus granulosus of buffalo in India: partial characterization of excretory–secretory and germinal membrane antigens. Veterinary Research Communications 31, 457–460.CrossRefGoogle ScholarPubMed
Martinez, J., Perez-Serrano, J., Bernadina, W.E. & Rodriguez-Caabeiro, F. (1999a) Echinococcus granulosus: in vitro effects of ivermectin and praziquantel on hsp60 and hsp70 levels. Experimental Parasitology 93, 171–180.CrossRefGoogle ScholarPubMed
Martinez, J., Perez-Serrano, J., Bodega, G., Casado, N. & Rodriguez-Caabeiro, F. (1999b) Heat shock proteins hsp70 and hsp60 in Echinococcus granulosus protoscoleces. Folia Parasitologica 46, 76–78.Google Scholar
Reddy, Y.A., Rao, J.R., Butchaiah, G. & Sharma, B. (1998) Random amplified polymorphic DNA for the specific detection of bubaline Echinococcus granulosus by hybridization assay. Veterinary Parasitology 79, 315–323.CrossRefGoogle ScholarPubMed
Singh, B.P., Joshi, P. & Singh, L.N. (1992) Electrophoretic and immunological differentiation of Indian buffalo and sheep hydatidosis. Indian Journal of Parasitology 16, 105–109.Google Scholar