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Sodium dodecyl sulphate as a rapid clearing agent for studying the hard parts of monogeneans and nematodes

Published online by Cambridge University Press:  12 April 2024

W.L. Wong ,
W.B. Tan  and
L.H.S. Lim
W.L. Wong
Affiliation:
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
W.B. Tan
Affiliation:
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
L.H.S. Lim*
Affiliation:
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
*
*Author for correspondence Fax: +603 7967 4178 E-mail: [email protected]
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Abstract

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Hard structures of helminths have often been used for taxonomic identification but are usually not clearly defined when treated with conventional methods such as ammonium picrate-glycerin for monogeneans and glycerin for nematodes. The present study reports a rapid and simple technique to better resolve the hard parts of selected monogeneans and nematodes using 5–10% alkaline sodium dodecyl sulphate (SDS). In comparison with established methods, SDS-treated worms become more transparent. In monogeneans treated with SDS, clear details of the hooks, hook filaments, anchors, bars and the sclerotized copulatory organs could be observed. In SDS-treated nematodes, spicules and ornamentations of the buccal capsules could be clearly seen.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 1 , March 2006 , pp. 87 - 90
Copyright
Copyright © Cambridge University Press 2006

References

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