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A simplified radioimmunoassay technique for hydatid disease and human trichinosis

Published online by Cambridge University Press:  05 June 2009

R. M. Matossian
Affiliation:
Dept. of Microbiology, American University of Beirut, Beirut, Lebanon

Abstract

A. A comparative study of a simplified radioimmunoassay (RIA) technique with the indirect haemagglutination (IHA) test in the diagnosis of 72 patients with hydatid disease indicated the greater sensitivity of the former. Both methods had an equal specificity in 60 control patients. RIA will serve as a useful addition to tests already available in hydatid serology.

B. A comparison of the (RIA) technique with the fluorescent antibody test (FAT) in the diagnosis of 33 patients with early, active trichinosis and 30 healthy controls indicated an equal sensitivity and specificity of both methods. The adoption of RIA as an easier method is recommended.

This study of a simplified radioimmunoassay technique for the diagnosis of hydatid disease and trichinosis in man is presented in two sections.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Garabedian, G.A.(1971) Evaluation of the reactivity of hydatid whole scolex antigen in hydatid disease serology. Annals of Tropical Medicine and Parasitotogy. 65, 385391.Google Scholar
Huldt, G., Johansson, S.G.O. & Lantto, S. (1973) Echinococcosis in Northern Scandinavia. Immune reactions to Echinococcus granulosus in Kautokeino Lapps. Archives of Environmental Health, 26, 3640.CrossRefGoogle ScholarPubMed
Matossian, R.M., Rebeiz, J. & Stephan, E.(1975)Serodiagnosis by fluorescent antibody stainingof an outbreak of trichinosis in Lebanon. Annals of Tropical Medicine and Parasitology, 69, 387392.CrossRefGoogle Scholar
Matossian, R.M. & Araj, G.F. (1975) Serologic evidence of the postoperative persistence of hydatid cysts in man. Journal of Hygiene, 75, 333340.CrossRefGoogle ScholarPubMed
Matossian, R.M. (1977) The immunological diagnosis of human hydatid disease. Transactions of the Royal Society of Tropical Medicine and Hygiene, 71, 101104.CrossRefGoogle ScholarPubMed
Matossian, R.M., McLaren, M.L., Draper, C.C., Bradstreet, G.M., Dighero, M.W., Kane, G.J., Mackinlay, L.M. & Rickard, M.D. (1979) The sero-diagnosis of human hydatid disease. 2. Additional studies on selected sera using indirect haemagglutination (IHA), enzyme linked immunosorbent assay (ELISA) and defined mass antigen substrate spheres (DASS), Journal of Helminthology, 53, 287291.Google Scholar
Musioni, P., Piontelli, M., Arru, E. & Pozzuoli, R. (1975) A solid phase radioimmunoassay for the diagnosis of human hydatidosis. Journal of Immunology, 112, 16741679.Google Scholar
Voller, A, Bidwell, D.E., Bartlett, A. & Edwards, R. (1977) A comparison of isotopic and enzyme-immunoassays for tropical parasitic diseases. Transactions of the Royal Society of Tropical Medicine and Hygiene, 71, 431437.CrossRefGoogle ScholarPubMed
Yallow, R.S. (1978) Radioimmunoassay: A probe for the fine structure of biologic systems. Science, 200, 12361245.CrossRefGoogle Scholar