Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-24T02:57:16.597Z Has data issue: false hasContentIssue false
  • English
  • Français

Relative expression of antigen B coding gene of bubaline isolates of Echinococcus granulosus in fertile and sterile cysts

Published online by Cambridge University Press:  28 September 2009

D. Pan ,
A.K. Bera ,
S. Bandyopadhyay ,
S.K. Das ,
S. Bandyopadhyay ,
S. Bhattacharyya ,
B. Manna ,
S. De  and
D. Bhattacharya
D. Pan
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
A.K. Bera
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
S. Bandyopadhyay
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
S.K. Das
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
S. Bandyopadhyay
Affiliation:
NRC on Yak, Dirang790 101, Arunachal Pradesh, India
S. Bhattacharyya
Affiliation:
Department of Genetics, Bidhan Chandra Krishi Viswavidyalaya, Nadia741 252, West Bengal, India
B. Manna
Affiliation:
Department of Zoology, Ballygunge Science College, University of Calcutta, Kolkata700 019, West Bengal, India
S. De
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
D. Bhattacharya*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
*
*E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

This article communicates the relative quantification of five isoforms of antigen B (AgB) of Echinococcus granulosus. Relative expression of the AgB was quantified in active and inactive cysts. Cysts with germinal membrane, clear cyst fluid and protoscoleces showed uniform expression of the five isoforms and were utilized as control. Relative expression of AgB1 was the highest in cysts, where calcification has initiated. AgB2 and AgB4 were expressed more in fertile cysts irrespective of the condition of germinal membrane. The lowest expression of AgB3 was seen in calcified cysts. The relative expression of AgB5 could not be correlated with respect to the condition of the cyst because AgB5 is typically expressed by the adult stage of the parasite.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 3 , September 2010 , pp. 241 - 244
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arend, A.C., Zaha, A., Ayala, F.J. & Haag, K.L. (2004) The Echinococcus granulosus antigen B shows a high degree of genetic variability. Experimental Parasitology 108, 76–80.CrossRefGoogle Scholar
Chemale, G., Haag, K.L., Ferreira, H.B. & Zaha, A. (2001) Echinococcus granulosus antigen B is encoded by a gene family. Molecular and Biochemical Parasitology 116, 233–237.CrossRefGoogle ScholarPubMed
Chemale, G., Ferreira, H.B., Barrett, J., Brophy, P.M. & Zaha, A. (2005) Echinococcus granulosus antigen B hydrophobic ligand binding properties. Biochimica Biophysica Acta 1747, 189–194.CrossRefGoogle ScholarPubMed
Eckert, J. & Thompson, R.C. (1997) Intraspecific variation of Echinococcus granulosus and related species with emphasis on their infectivity to humans. Acta Tropica 64, 19–34.CrossRefGoogle ScholarPubMed
Fernández, V., Ferreira, H.B., Fernández, C., Zaha, A. & Nieto, A. (1996) Molecular characterisation of a novel 8-kDa subunit of Echinococcus granulosus antigen B. Molecular and Biochemical Parasitology 77, 247–250.CrossRefGoogle ScholarPubMed
Frosch, P., Hartmann, M., Mühlschlegel, F. & Frosch, M. (1994) Sequence heterogeneity of the echinococcal antigen B. Molecular and Biochemical Parasitology 64, 171–175.CrossRefGoogle ScholarPubMed
Guarnera, E.A., Parra, A., Kamenetzky, L., García, G. & Gutiérrez, A. (2004) Cystic echinococcosis in Argentina: evolution of metacestode and clinical expression in various Echinococcus granulosus strains. Acta Tropica 92, 153–159.CrossRefGoogle ScholarPubMed
Haag, K.L., Alves-Junior, L., Zaha, A. & Ayala, F.J. (2004) Contingent, non-neutral evolution in a multicellular parasite: natural selection and gene conversion in the Echinococcus granulosus antigen B gene family. Gene 333, 157–167.CrossRefGoogle Scholar
Haag, K.L., Zanotto, P.M., Alves-Junior, L., Gasser, R.B., Zaha, A. & Ayala, F.J. (2006) Searching for antigen B genes and their adaptive sites in distinct strains and species of the helminth Echinococcus. Infection Genetics and Evolution 6, 251–261.CrossRefGoogle ScholarPubMed
Ibrahem, M.M., Craig, P.S., McVie, A., Ersfeld, K. & Rogan, M.T. (1996) Echinococcus granulosus antigen B and seroreactivity in natural ovine hydatidosis. Research in Veterinary Science 61, 102–106.CrossRefGoogle ScholarPubMed
Ito, A., Ma, L., Schantz, P.M., Gottstein, B., Liu, Y.H., Chai, J.J., Abdel-Hafez, S.K., Altintas, N., Joshi, D.D., Lightowlers, M.W. & Pawlowski, Z.S. (1999) Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18). American Journal of Tropical Medicine Hygiene 60, 188–192.CrossRefGoogle Scholar
Kamenetzky, L., Muzulin, P.M., Gutierrez, A.M., Angel, S.O., Zaha, A., Guarnera, E.A. & Rosenzvit, M.C. (2005) High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus. Parasitology 131, 805–815.CrossRefGoogle ScholarPubMed
Livak, K.J. (1997 and 2000) ABI prism 7700 sequence detection system user bulletin # 2 relative quantification of gene expression. Available at websitehttp/docs.appliedbiosystems.com/pebiodocs/04303859.pdf (accessed 2006).Google Scholar
Livak, K.J. & Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2( − Delta Delta C(T)) method. Methods 25, 402–408.CrossRefGoogle ScholarPubMed
Maddison, S.E., Slemenda, S.B., Schantz, P.M., Fried, J.A., Wilson, M. & Tsang, V.C. (1989) A specific diagnostic antigen of Echinococcus granulosus with an apparent molecular weight of 8 kDa. American Journal of Tropical Medicine and Hygiene 40, 377–383.CrossRefGoogle Scholar
Mamuti, W., Yamasaki, H., Sako, Y., Nakaya, K., Nakao, M., Lightowlers, M.W. & Ito, A. (2002) Usefulness of hydatid cyst fluid of Echinococcus granulosus developed in mice with secondary infection for serodiagnosis of cystic Echinococcosis in humans. Clinical and Diagnostic Laboratory Immunology 9, 573–576.Google ScholarPubMed
Mamuti, W., Sako, Y., Xiao, N., Nakaya, K., Nakao, M., Yamasaki, H., Lightowlers, M.W., Craig, P.S. & Ito, A. (2006) Echinococcus multilocularis: developmental stage-specific expression of antigen B 8-kDa-subunits. Experimental Parasitology 113, 75–82.CrossRefGoogle ScholarPubMed
Pawlowski, Z.S., Eckert, J., Vuitton, D.A., Ammann, R.W., Kern, P., Craig, P.S., Dar, K.F., Rosa, F.De, Filice, C., Gottstein, B., Grimm, F., Macpherson, C.N.L., Sato, N., Todordov, T., Uchino, J., von Sinner, W. & Wen, H. (2001) Echinococcosis in humans: clinical aspects, diagnosis and treatment. pp. 20–71in WHO/OIE manual on Echinococcosis in humans and animals: a public health problem of global concern. Paris, World Organization for Animal Health.Google Scholar
Rosenzvit, M.C., Camicia, F., Kamenetzky, L., Muzulin, P.M. & Gutierrez, A.M. (2006) Identification and intra-specific variability analysis of secreted and membrane-bound proteins from Echinococcus granulosus. Parasitology International 55, S63–S67.CrossRefGoogle ScholarPubMed
Shepherd, J.C., Aitken, A. & McManus, D.P. (1991) A protein secreted in vivo by Echinococcus granulosus inhibits elastase activity and neutrophil chemotaxis. Molecular and Biochemical Parasitology 44, 81–90.CrossRefGoogle ScholarPubMed
Siracusano, A., Margutti, P., Delunardo, F., Profumo, E., Rigano, R., Buttari, B., Teggi, A. & Ortona, E. (2008) Molecular cross-talk in host–parasite relationships: the intriguing immunomodulatory role of Echinococcus antigen B in cystic echinococcosis. International Journal for Parasitology 38, 1371–1376.CrossRefGoogle ScholarPubMed
Thompson, R.C.A. (1995) Biology and systematics of Echinococcus. pp. 411–463in Thompson, R.C.A. & Lymbery, A.J. (Eds) Echinococcus and hydatid disease. Wallingford, CAB International.Google Scholar
Williams, J.F., Pérez Esandi, M.V. & Oriol, R. (1971) Evaluation of purified lipoprotein antigens of Echinococcus granulosus in the immunodiagnosis of human infection. American Journal of Tropical Medicine and Hygiene 20, 575–579.CrossRefGoogle ScholarPubMed